GABAA receptor-expressing neurons promote consumption in Drosophila melanogaster

Cheung, Samantha K.; Scott, Kristin

doi:10.1371/journal.pone.0175177

Cited by 20 publications

(14 citation statements)

References 29 publications

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“…Interestingly, changing the ion-selectivity of an inhibitory anion channel into an excitatory cation channel led to a switch behaviour in the worm Caenorhabditis elegans (Pirri et al , 2015), demonstrating how the balance of excitatory vs inhibitory synapses may profoundly affect animal decisions. Likewise, knockdown of a GABA receptor, in Drosophila increased the consumption of non-appetitive and appetitive substances, beyond satiation (Cheung and Scott, 2017).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach

Varaldi

Lepetit

2018

Parasitology

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Behaviour manipulation imposed by parasites is a fascinating phenomenon but our understanding is still very limited. We studied the interaction between a virus and the parasitic wasp Leptopilina boulardi that attacks Drosophila larvae. Wasps usually refrain to lay eggs into already parasitized hosts (superparasitism avoidance). On the contrary, females infected by the Leptopilina boulardi Filamentous Virus (LbFV) are much more incline to superparasitize. Interestingly, the host-sharing induced by this behaviour modification leads to the horizontal transmission of the virus, thus increasing its fitness at the expense of that of the wasp. To better understand the mechanisms underlying this behaviour manipulation, we studied by RNA sequencing the meta-transcriptome of LbFV and the parasitic wasp both in the abdomen and in the head. We found that the abundance of viral transcripts was independent of the wasp strain but strongly differed between tissues. Based on the tissue pattern of expression, we identified a set of 20 viral genes putatively involved in the manipulation process. In addition, we identified a set of wasp genes deregulated in the presence of the virus either in the abdomen or in the head, including genes with annotations suggesting involvement in behaviour (i.e. Potassium-channel protein). This dataset gives new insights into the behaviour manipulation and on the genetic basis of superparasitism in parasitoids.

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Section: Discussionmentioning

confidence: 99%

Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach

Varaldi

Lepetit

2018

Parasitology

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“…Insect Rdl GABA receptors are involved in a wide variety of physiological processes, such as walking (Gowda et al, ), mating song response behavior (Li, Ishimoto, & Kamikouchi, ; Yamada et al, ), mating competitiveness (Platt et al, ), substance consumption (Cheung & Scott, ), aggression (Yuan, Song, Yang, Jan, & Jan., ), visual processing (Freifeld, Clark, Schnitzer, Horowitz, & Clandinin, ), olfactory habituation (Das et al, ; Sudhakaran et al, ), olfactory learning (X. Liu & Davis, ; X. Liu, Buchanan, Han, & Davis, ; X. Liu, Krause, & Davis, ), visual reversal learning (Ren, Li, Wu, Ren, & Guo, ; Wu, Ren, Li, & Guo, ), rest and arousal regulation (S. Liu et al, ; McCarthy et al, ; Parisky et al, ), and sleep (Agosto et al, ; Chung, Kilman, Keath, Pitman, & Allada, ; Seugnet, Dissel, Thimgan, Cao, & Shaw, ).…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporally different expression of alternatively spliced GABA receptor subunit transcripts in the housefly Musca domestica

Kita

Mino

Ozoe

et al. 2019

Arch Insect Biochem Physiol

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Insect γ-aminobutyric acid (GABA) receptors are important as major inhibitory neurotransmitter receptors and targets for insecticides. The housefly GABA receptor subunit gene MdRdl is alternatively spliced at exons 3 (a or b) and 6 (c or d) to yield the variants of ac, ad, bc, and bd combinations. In the present study, the expression of the MdRdl transcript in the body parts and in the developmental stages of the housefly Musca domestica was examined by quantitative polymerase chain reaction using specific primers that amplify the combinations of alternative exons. The results indicated that the transcripts of MdRdl, including four combinations, were highly expressed in the adult stage. MdRdl bd was the most abundant in the adult head. The expression pattern did not change in the adult stage over 7 days after eclosion. The expression level of the MdRdl bd transcript in the female head was similar to that of the male head. In contrast, MdRdl bc was the predominant transcript in the pupal head and the adult leg. Because the homomeric Rdl bc GABA receptor has a high affinity for GABA, our results provide grounds for designing agonist or competitive-antagonist insecticides that target the orthosteric site of the GABA receptor containing this Rdl variant. K E Y W O R D S alternative splicing, GABA, γ-Aminobutyric acid receptor, housefly, Rdl, spatiotemporal expression Arch. Insect Biochem. Physiol. 2019;101:e21541.wileyonlinelibrary.com/journal/arch

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“…see refs. 48 – 50 ), the link between Gr5a sense cells and the E49 motoneurons remains to be established.…”

Section: Discussionmentioning

confidence: 99%

Targeted kinase inhibition relieves slowness and tremor in a Drosophila model of LRRK2 Parkinson’s disease

Cording

Shiaelis

Petridi

et al. 2017

npj Parkinson's Disease

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In a number of Drosophila models of genetic Parkinson’s disease (PD) flies climb more slowly than wild-type controls. However, this assay does not distinguish effects of PD-related genes on gravity sensation, “arousal”, central pattern generation of leg movements, or muscle. To address this problem, we have developed an assay for the fly proboscis extension response (PER). This is attractive because the PER has a simple, well-identified reflex neural circuit, in which sucrose sensing neurons activate a pair of “command interneurons”, and thence motoneurons whose activity contracts the proboscis muscle. This circuit is modulated by a single dopaminergic neuron (TH-VUM). We find that expressing either the G2019S or I2020T (but not R1441C, or kinase dead) forms of human LRRK2 in dopaminergic neurons reduces the percentage of flies that initially respond to sucrose stimulation. This is rescued fully by feeding l-DOPA and partially by feeding kinase inhibitors, targeted to LRRK2 (LRRK2-IN-1 and BMPPB-32). High-speed video shows that G2019S expression in dopaminergic neurons slows the speed of proboscis extension, makes its duration more variable, and increases the tremor. Testing subsets of dopaminergic neurons suggests that the single TH-VUM neuron is likely most important in this phenotype. We conclude the Drosophila PER provides an excellent model of LRRK2 motor deficits showing bradykinesia, akinesia, hypokinesia, and increased tremor, with the possibility to localize changes in neural signaling.

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GABAA receptor-expressing neurons promote consumption in Drosophila melanogaster

Cited by 20 publications

References 29 publications

Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach

Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach

Spatiotemporally different expression of alternatively spliced GABA receptor subunit transcripts in the housefly Musca domestica

Targeted kinase inhibition relieves slowness and tremor in a Drosophila model of LRRK2 Parkinson’s disease

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