GABAA Receptors are Selectively Expressed in NG2 Glia of the Cerebellar White Matter

Labrada-Moncada, Francisco Emmanuel; Martı́nez-Torres, Ataúlfo; Reyes‐Haro, Daniel

doi:10.1016/j.neuroscience.2020.03.003

Cited by 9 publications

(13 citation statements)

References 44 publications

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“…For example, building on the potential role of OPCs in regulating the status of the BBB, tissue-specific ablation of TGF-β1 in mice ( Pdgfrα-Cre; Tgfb1 flox ) resulted in loss of BBB function and cerebral hemorrhage (Seo et al, 2014 ). Other work has further refined the function of OPC-neuronal crosstalk, as previously discussed (Birey et al, 2015 ; Labrada-Moncada et al, 2020 ). From such studies, we know that OPCs express ion-channels and neurotransmitter receptors which allow them to detect neuronal activity (Larson et al, 2016 ) and, in turn, modulate neuronal behavior (Wang and Young, 2014 ; Douglas Fields, 2015 ; Purger et al, 2016 ).…”

Section: Genetic Technologiesmentioning

confidence: 87%

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Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease

Galichet

Clayton

Lovell‐Badge

2021

Front. Cell. Neurosci.

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Oligodendrocyte progenitor cells (OPCs), also referred to as NG2-glia, are the most proliferative cell type in the adult central nervous system. While the primary role of OPCs is to serve as progenitors for oligodendrocytes, in recent years, it has become increasingly clear that OPCs fulfil a number of other functions. Indeed, independent of their role as stem cells, it is evident that OPCs can regulate the metabolic environment, directly interact with and modulate neuronal function, maintain the blood brain barrier (BBB) and regulate inflammation. In this review article, we discuss the state-of-the-art tools and investigative approaches being used to characterize the biology and function of OPCs. From functional genetic investigation to single cell sequencing and from lineage tracing to functional imaging, we discuss the important discoveries uncovered by these techniques, such as functional and spatial OPC heterogeneity, novel OPC marker genes, the interaction of OPCs with other cells types, and how OPCs integrate and respond to signals from neighboring cells. Finally, we review the use of in vitro assay to assess OPC functions. These methodologies promise to lead to ever greater understanding of this enigmatic cell type, which in turn will shed light on the pathogenesis and potential treatment strategies for a number of diseases, such as multiple sclerosis (MS) and gliomas.

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Section: Genetic Technologiesmentioning

confidence: 87%

“…OPCs also receive inhibitory neuronal input (Lin and Bergles, 2004 ; Kukley et al, 2008 ), which, at least in the mouse cerebellum, is mediated through GABA A receptors (Labrada-Moncada et al, 2020 ); however the functional consequence of this interaction remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease

Galichet

Clayton

Lovell‐Badge

2021

Front. Cell. Neurosci.

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“…Brain slices were obtained as previously described (Reyes-Haro et al, 2010Labrada-Moncada et al, 2020). Briefly, acute cerebellar slices were prepared from 8-to 10-day-old (P8-P10) CD-1 or GFAP-eGFP mice (Nolte et al, 2001).…”

Section: Acute Brain Slice Preparation and Calcium Imagingmentioning

confidence: 99%

A Functional Signature in the Developing Cerebellum: Evidence From a Preclinical Model of Autism

Soria-Ortiz

Reyes-Ortega

Martı́nez-Torres

et al. 2021

Front. Cell Dev. Biol.

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Autism spectrum disorders (ASD) are pervasive neurodevelopmental conditions detected during childhood when delayed language onset and social deficits are observed. Children diagnosed with ASD frequently display sensorimotor deficits associated with the cerebellum, suggesting a dysfunction of synaptic circuits. Astroglia are part of the tripartite synapses and postmortem studies reported an increased expression of the glial fibrillary acidic protein (GFAP) in the cerebellum of ASD patients. Astroglia respond to neuronal activity with calcium transients that propagate to neighboring cells, resulting in a functional response known as a calcium wave. This form of intercellular signaling is implicated in proliferation, migration, and differentiation of neural precursors. Prenatal exposure to valproate (VPA) is a preclinical model of ASD in which premature migration and excess of apoptosis occur in the internal granular layer (IGL) of the cerebellum during the early postnatal period. In this study we tested calcium wave propagation in the IGL of mice prenatally exposed to VPA. Sensorimotor deficits were observed and IGL depolarization evoked a calcium wave with astrocyte recruitment. The calcium wave propagation, initial cell recruitment, and mean amplitude of the calcium transients increased significantly in VPA-exposed mice compared to the control group. Astrocyte recruitment was significantly increased in the VPA model, but the mean amplitude of the calcium transients was unchanged. Western blot and histological studies revealed an increased expression of GFAP, higher astroglial density and augmented morphological complexity. We conclude that the functional signature of the IGL is remarkably augmented in the preclinical model of autism.

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“…Brain slices were obtained as previously described (Labrada-Moncada et al, 2020;Reyes-Haro et al, 2010. Briefly, acute cerebellar slices were prepared from 7-to 9-day-old (P7-P9) CD-1 or GFAP-eGFP mice (Nolte et al, 2001).…”

Section: Acute Brain Slice Preparation and Calcium Imagingmentioning

confidence: 99%

“…The cells recruited within the calcium wave were visualized after subtraction of pixel values from the previous image. All the recruited cells were displayed after adding up all the images with the corresponding background subtraction (Haas et al, 2006;Labrada-Moncada et al, 2020;Reyes-Haro et al, 2010). The functional extension (maximum length) and number of recruited cells within the calcium wave were estimated using concentric rings with 50 µm increments around the site of stimulation.…”

Section: Acute Brain Slice Preparation and Calcium Imagingmentioning

confidence: 99%

A functional network signature in the developing cerebellum: evidence from a preclinical model of autism

Soria-Ortiz

Martı́nez-Torres

Reyes‐Haro

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Autism spectrum disorders (ASD) are pervasive neurodevelopmental conditions detected during childhood when delayed language onset and social deficits are observed. Children diagnosed with ASD frequently display sensorimotor deficits associated with the cerebellum, suggesting a dysfunction of synaptic circuits. Astroglia are part of the tripartite synapses and postmortem studies reported an increased expression of the glial fibrillary acidic protein (GFAP) in the cerebellum of ASD patients. Astroglia respond to neuronal activity with calcium transients that propagate to neighboring cells, resulting in a functional network response known as a calcium wave. This form of intercellular signaling is implicated in proliferation, migration, and differentiation of neural precursors. Prenatal exposure to valproate (VPA) is a preclinical model of ASD in which premature migration and excess of apoptosis occur in the internal granular layer (IGL) of the cerebellum during the early postnatal period. In this study we tested calcium wave propagation in the IGL of mice prenatally exposed to VPA. Sensorimotor and social deficits were observed and IGL depolarization evoked a calcium wave with astrocyte recruitment. The calcium wave propagation, initial cell recruitment, and mean amplitude of the calcium transients increased significantly in VPA-exposed mice compared to the control group. Astrocyte recruitment was significantly increased in the VPA model, but the mean amplitude of the calcium transients was unchanged. Western blot and histological studies revealed an increased expression of GFAP and higher astroglial density. We conclude that the functional network of the IGL is remarkably augmented in the preclinical model of autism.

show abstract

GABAA Receptors are Selectively Expressed in NG2 Glia of the Cerebellar White Matter

Cited by 9 publications

References 44 publications

Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease

Novel Tools and Investigative Approaches for the Study of Oligodendrocyte Precursor Cells (NG2-Glia) in CNS Development and Disease

A Functional Signature in the Developing Cerebellum: Evidence From a Preclinical Model of Autism

A functional network signature in the developing cerebellum: evidence from a preclinical model of autism

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