2021
DOI: 10.1186/s13041-021-00746-1
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GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter

Abstract: GABAergic interneurons play a critical role in tuning neural networks in the central nervous system, and their defects are associated with neuropsychiatric disorders. Currently, the mDlx enhancer is solely used for adeno-associated virus (AAV) vector-mediated transgene delivery into cortical interneurons. Here, we developed a new inhibitory neuron-specific promoter (designated as the mGAD65 promoter), with a length of 2.5 kb, from a mouse genome upstream of exon 1 of the Gad2 gene encoding glutamic acid decarb… Show more

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Cited by 37 publications
(36 citation statements)
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“…Direct injection of rAAVs carrying I56i, h12R, or h56D into the murine cerebral cortex and hippocampus resulted in the transduction of 80–90% of cortical and hippocampal GABAergic neurons after each injection, respectively ( 12 , 14 ). Intravenous injection of rAAV-I56i labeled 57% of murine cortical GABAergic neurons ( 10 ). All of the aforementioned DLX enhancers (I12b, I56i, h12R, and h56D) directed transgene expression to murine cortical and hippocampal GABAergic neurons with approximately 90% or greater specificity [( 10 12 , 14 ); see Table 1 ].…”
Section: The DLX Gene Enhancer Elementsmentioning
confidence: 99%
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“…Direct injection of rAAVs carrying I56i, h12R, or h56D into the murine cerebral cortex and hippocampus resulted in the transduction of 80–90% of cortical and hippocampal GABAergic neurons after each injection, respectively ( 12 , 14 ). Intravenous injection of rAAV-I56i labeled 57% of murine cortical GABAergic neurons ( 10 ). All of the aforementioned DLX enhancers (I12b, I56i, h12R, and h56D) directed transgene expression to murine cortical and hippocampal GABAergic neurons with approximately 90% or greater specificity [( 10 12 , 14 ); see Table 1 ].…”
Section: The DLX Gene Enhancer Elementsmentioning
confidence: 99%
“…Intravenous injection of rAAV-I56i labeled 57% of murine cortical GABAergic neurons ( 10 ). All of the aforementioned DLX enhancers (I12b, I56i, h12R, and h56D) directed transgene expression to murine cortical and hippocampal GABAergic neurons with approximately 90% or greater specificity [( 10 12 , 14 ); see Table 1 ]. Similar levels of GABAergic neuronal specificity and coverage were observed in gerbils and in non-human primates ( 12 , 14 ).…”
Section: The DLX Gene Enhancer Elementsmentioning
confidence: 99%
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