GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed by<i>In Vivo</i>Functional Ca<sup>2+</sup>Imaging in Transgenic Mice

Sohya, Kazuhiro; Kameyama, Katsuro; Yanagawa, Yuchio; Obata, Kunihiko; Tsumoto, Tadaharu

doi:10.1523/jneurosci.4641-06.2007

Cited by 219 publications

(242 citation statements)

References 32 publications

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“…After electrophysiological recordings, slices containing biocytin-loaded neurons were processed in a manner similar to that described previously (Jiang et al, 2004) and observed with the two-photon laser-scanning microscope system (Sohya et al, 2007) for imaging the labeled neurons.…”

Section: Methodsmentioning

confidence: 99%

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Jiang¹,

Sohya²,

Sarihi³

et al. 2010

J. Neurosci.

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The developmental period when neuronal responses are modified by visual experience is reported to start and end earlier in layer 4 than in layer 2/3ofthevisualcortex,andthematurationofGABAergicinhibitorycircuitsissuggestedtodeterminethetimingofthisperiod.Here,weexamine whether the laminar difference in such timing corresponds to a difference in the time course of the functional maturation of GABAergic synaptic transmission to star pyramidal and pyramidal cells in layers 4 and 2/3, respectively, of the mouse visual cortex and whether the development of the strength of GABAergic transmission is affected by visual deprivation in a laminar-specific manner. Our analysis of developmental changes in inhibitory postsynaptic currents of star pyramidal and pyramidal cells evoked by electrical stimulation of afferents or action potentials of fast-spiking GABAergic neurons revealed that there was a sequential maturation of GABAergic function from layers 4 to 2/3. The maturation of inhibition in layer 4 occurred at postnatal week 3, which preceded by 1 week that of layer 2/3. Visual deprivation by dark rearing arrested the functional development of GABAergic transmission in layer 2/3, whereas dark rearing was not so effective in layer 4. GABAergic synapses in layer 2/3 were sensitive to an agonist for cannabinoid type 1 receptors and not normally matured in receptor knock-out mice, whereas those in layer 4werenotso.Theseresultssuggestlaminar-specificmaturationofinhibitionandsusceptibilitytovisualdeprivation,whichmayberelatedtothe laminar difference in sensitivity to endocannabinoids.

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Section: Methodsmentioning

confidence: 99%

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Jiang¹,

Sohya²,

Sarihi³

et al. 2010

J. Neurosci.

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“…Subsequently, the slices were incubated in avidind-rhodamine (Pierce, Rockford, IL; diluted 1:400 in the Triton X-100-containing PBS) for 24 h, and finally washed in 0.1 M PBS five to seven times each for 10 min. For imaging labeled neurons, slices were placed on a chamber containing 0.1 M PBS and observed with two-photon laser scanning microscopy (Radiance 2000MP; BioRad, Hertfordshire, UK) (Sohya et al, 2007). Excitation light was focused using a 40ϫ waterimmersion objective (0.8 numerical aperture; Nikon, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Metabotropic Glutamate Receptor Type 5-Dependent Long-Term Potentiation of Excitatory Synapses on Fast-Spiking GABAergic Neurons in Mouse Visual Cortex

Sarihi¹,

Jiang²,

Komaki‬³

et al. 2008

J. Neurosci.

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“…A recently developed extracellular injection technique has made it possible to load all or nearly all neurons in a 200-to 300-m region of cortex with a cell-permeant version of the calcium indicator OregonGreen BAPTA-1 (OGB-1) (13,14). This technique has been applied successfully to the study of neuronal response properties in rodent visual cortex (15)(16)(17).…”

Section: Imaging Responses In Labeled Inhibitorymentioning

confidence: 99%

“…The standard procedure in ocular dominance studies of single cells is first to find a cell's optimal visual stimulus and then to compare the responses to this optimal stimulus when it is presented to each eye separately. This strategy is ill-suited to parallel recording because neurons in mouse visual cortex are highly selective for the orientation and spatial and temporal frequency of the stimulus (18,19,22,23) and because cells with similar stimulus selectivity are not spatially organized (15)(16)(17). For example, by using a set of eight oriented drifting gratings whose spatial and temporal characteristics were chosen to excite the largest number of mouse V1 neurons, each repeated 10 times, we could obtain a reliable response in only 48% of cells (bootstrapped t test; P Ͻ 0.01) [supporting information (SI) Fig.…”

Section: Imaging Responses In Labeled Inhibitorymentioning

confidence: 99%

Delayed plasticity of inhibitory neurons in developing visual cortex

Gandhi

Yanagawa

Stryker

2008

Proc. Natl. Acad. Sci. U.S.A.

105

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During postnatal development, altered sensory experience triggers the rapid reorganization of neuronal responses and connections in sensory neocortex. This experience-dependent plasticity is disrupted by reductions of intracortical inhibition. Little is known about how the responses of inhibitory cells themselves change during plasticity. We investigated the time course of inhibitory cell plasticity in mouse primary visual cortex by using functional two-photon microscopy with single-cell resolution and genetic identification of cell type. Initially, local inhibitory and excitatory cells had similar binocular visual response properties, both favoring the contralateral eye. After 2 days of monocular visual deprivation, excitatory cell responses shifted to favor the open eye, whereas inhibitory cells continued to respond more strongly to the deprived eye. By 4 days of deprivation, inhibitory cell responses shifted to match the faster changes in their excitatory counterparts. These findings reveal a dramatic delay in inhibitory cell plasticity. A minimal linear model reveals that the delay in inhibitory cell plasticity potently accelerates Hebbian plasticity in neighboring excitatory neurons. These findings offer a network-level explanation as to how inhibition regulates the experiencedependent plasticity of neocortex.inhibition ͉ ocular dominance ͉ two-photon microscopy ͉ calcium imaging ͉ cortical plasticity

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GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed byIn VivoFunctional Ca²⁺Imaging in Transgenic Mice

Cited by 219 publications

References 32 publications

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Metabotropic Glutamate Receptor Type 5-Dependent Long-Term Potentiation of Excitatory Synapses on Fast-Spiking GABAergic Neurons in Mouse Visual Cortex

Delayed plasticity of inhibitory neurons in developing visual cortex

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GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed byIn VivoFunctional Ca2+Imaging in Transgenic Mice

Cited by 219 publications

References 32 publications

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex

Metabotropic Glutamate Receptor Type 5-Dependent Long-Term Potentiation of Excitatory Synapses on Fast-Spiking GABAergic Neurons in Mouse Visual Cortex

Delayed plasticity of inhibitory neurons in developing visual cortex

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GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed byIn VivoFunctional Ca²⁺Imaging in Transgenic Mice