GABARAPL1 suppresses metastasis by counteracting PI3K/Akt pathway in prostate cancer

Su, Wei; Li, Shibao; Chen, Xiaofan; Yin, Lingyu; Ma, Ping; Ma, Yingyu; Su, Bing

doi:10.18632/oncotarget.13879

Cited by 24 publications

(22 citation statements)

References 39 publications

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“…Ta 3 N 5 suffers from very low carrier transport properties and fast carrier recombination (<10 ps) in the absence of surface modification despite its suitable absorption in the visible-light range. 15 Several studies have focused on modifying Ta 3 N 5 photocatalyst surfaces with cocatalysts, [15][16][17][18][19] e.g., cobalt oxide. 19 A highly active OER electrocatalyst is desirable as a catalyst for a successful photocatalytic water splitting reaction.…”

Section: -8mentioning

confidence: 99%

“…15 Several studies have focused on modifying Ta 3 N 5 photocatalyst surfaces with cocatalysts, [15][16][17][18][19] e.g., cobalt oxide. 19 A highly active OER electrocatalyst is desirable as a catalyst for a successful photocatalytic water splitting reaction. Recently, literature has re-4 discovered that the edge-sharing octahedral NiFeO x catalyst exhibits one of the highest OER activities in alkaline solution.…”

Section: -8mentioning

confidence: 99%

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Temperature Dependence of Electrocatalytic and Photocatalytic Oxygen Evolution Reaction Rates Using NiFe Oxide

et al. 2016

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The present work compares the oxygen evolution reaction (OER) in electrocatalysis and photocatalysis in aqueous solutions using nanostructured NiFeO x as catalysts. The impacts of pH and reaction temperature on the electrocatalytic and photocatalytic OER kinetics were investigated. For electrocatalysis, a NiFeO x catalyst was hydrothermally decorated on Ni foam. In 1 M KOH solution, the NiFeO x electrocatalyst achieved 10 mA cm −2 at an overpotential of 260 mV. The same catalyst was decorated on the surface of Ta 3 N 5 photocatalyst powder. The reaction was conducted in the presence of 0.1 M Na 2 S 2 O 8 as a strong electron scavenger, thus likely leading to the OER being kinetically relevant. When compared with the bare Ta 3 N 5 , NiFeO x /Ta 3 N 5 demonstrated a 5-fold improvement in photocatalytic activity in the OER under visible light irradiation, achieving a quantum efficiency of 24% at 480 nm. Under the conditions investigated, a strong correlation between the electrocatalytic and photocatalytic performances was identified: an improvement in electrocatalysis corresponded with an improvement in photocatalysis without altering the identity of the materials. The rate change at different pH was likely associated with electrocatalytic kinetics that accordingly influenced the photocatalytic rates. The sensitivity of the reaction rates with respect to the reaction temperature resulted in an apparent activation energy of 25 kJ mol −1 in electrocatalysis, whereas the apparent activation energy in photocatalysis was 16 kJ mol −1 . The origin of the difference in these activation energy values is likely attributed to the possible effects of temperature on the individual thermodynamic and kinetic parameters of the reaction process. The work described herein demonstrates a method of "transferring the knowledge of electrocatalysis to photocatalysis" as a strong tool to rationally and quantitatively understand the complex reaction schemes involved in photocatalytic reactions.

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Section: -8mentioning

confidence: 99%

Section: -8mentioning

confidence: 99%

Temperature Dependence of Electrocatalytic and Photocatalytic Oxygen Evolution Reaction Rates Using NiFe Oxide

et al. 2016

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“…On the other hand, this protein was shown to counteract PI3K/Akt pathway leading to suppression of metastasis 30 . Moreover, GABARAPL1 downregulation in prostate cancer tissues was associated with decreased disease‐free survival in prostate cancer patients 30 . The present study reveals that WA causes induction of GABARAPL1 and its knockdown inhibits WA‐mediated apoptosis.…”

Section: Discussionmentioning

confidence: 57%

“…Moreover, GABARAPL1 expression was negatively associated with 5 years survival in the Oncomine data set for prostate cancer 29 . On the other hand, this protein was shown to counteract PI3K/Akt pathway leading to suppression of metastasis 30 . Moreover, GABARAPL1 downregulation in prostate cancer tissues was associated with decreased disease‐free survival in prostate cancer patients 30 .…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective autophagy induction by withaferin A in prostate cancer cells involves GABARAPL1

Hahm

Singh

2020

Molecular Carcinogenesis

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Withaferin A (WA) is a naturally occurring steroidal lactone with proven cancer chemopreventive activity in preclinical models of different cancers including prostate adenocarcinoma. Previously we compared the RNA‐seq data from control and WA‐treated 22Rv1 human prostate cancer cells to identify mechanistic targets of this phytochemical. The Gene Ontology pathway analysis of the RNA‐seq data revealed significant upregulation of genes associated with autophagy upon WA treatment in 22Rv1 cells. In this study, we extended these findings to investigate the mechanism underlying WA‐induced autophagy. Initially, we confirmed autophagy induction by WA treatment by transmission electron microscopy using three prostate cancer cell lines (LNCaP, 22Rv1, and PC‐3). Fourteen common genes altered by 8‐ and 16‐hour exposure to WA were identified from human autophagy PCR array and these results were consistent with the RNA‐seq data. Two key autophagy markers (LC3BII and SQSTM1) were robustly increased in WA‐exposed LNCaP, 22Rv1, and PC‐3 cells as determined by immunoblotting, and this effect was elevated in the presence of autophagy inhibitor bafilomycin A1 (BafA1). BafA1 treatment augmented WA's cytotoxicity and subsequently its proapoptotic potential. WA treatment induced GABARAPL1 (ATG8L) protein expression in all three cell lines and its knockdown by RNA interference attenuated WA‐mediated apoptosis. WA‐induced autophagy was not affected in the presence of an antioxidant (EUK134). Taken together, the present study reveals that WA‐mediated autophagy is cytoprotective and mediated by GABARAPL1.

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“…This indicated that miR-375 was involved in regulating and fine adjustment to IL-13-mediated reactions. The PI3K-Akt signaling pathway has been reported to be closely associated with the growth of cells, such as proliferation, migration, invasion and metastasis in cancer (33)(34)(35), and certain other diseases, including diabetic kidney (36) and traumatic brain injury (37).…”

Section: Ppi Network and Module Constructionmentioning

confidence: 99%

Analysis of the function of microRNA-375 in humans using bioinformatics

Chen

Luo

et al. 2017

Biomedical Reports

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Abstract. ) is expressed at low levels in many types of solid tumor, particularly in gastrointestinal tumors. It is considered to be important in the development of cancer and certain diseases. Thus, more detailed knowledge is required on the particular functions of miR-375. miRs function by regulating target genes. Therefore, in the current study, miRWalk (which includes the data from 10 prediction software programs) was used to predict the target genes of miR-375. The genes, which were co-predicted using five different software programs were further analyzed using Database for Annotation, Visualization and Integrated Discovery online software [including gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis]. Subsequently, the online tool, Search Tool for the Retrieval of Interacting Genes, was used to analyze the protein-protein interaction and construct modules using Cytoscape. The result demonstrated 6,574 predicted genes, 1,325 of which were co-predicted. The GO analysis result indicated that, in biological processes, the co-predicted genes were significantly enriched in the regulation of nervous system development and cell differentiation, and the highest enrichment of molecular function was ion binding. In KEGG analysis, the genes were enriched in the Hippo signaling pathway, glutamatergic synapse, circadian entrainment and the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. The top 10 hub proteins were mechanistic target of rapamycin, PH domain and leucine rich repeat protein phosphatase 1, ubiquitously transcribed tetratricopeptide repeat containing, Y-linked, histone deacetylase 2, F-box and leucine rich repeat protein 19, KIT proto-oncogene receptor tyrosine kinase, angiotensinogen, Janus kinase 2, fibroblast growth factor 2 and RNA polymerase II subunit A. These proteins predominantly regulate the development and progression of cancer, hypertension, essential thrombocythemia and inflammation. The genes in the top seven modules selected were identified to be primarily enriched in chemokines, extracellular matrix-receptor interaction, focal adhesion, the PI3K-Akt signaling pathway, amoebiasis and protein processing signaling pathway. Thus, the target genes and hub proteins that were predicted in the current study were identified to be important in regulating the development and progression of cancer and certain diseases. Furthermore, they present potential novel biomarkers for tumor diagnosis and candidate targets for treatment, and indicate that further research is required to establish the functions of miR-375.

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GABARAPL1 suppresses metastasis by counteracting PI3K/Akt pathway in prostate cancer

Abstract: ABSTRACT

Cited by 24 publications

References 39 publications

Temperature Dependence of Electrocatalytic and Photocatalytic Oxygen Evolution Reaction Rates Using NiFe Oxide

Temperature Dependence of Electrocatalytic and Photocatalytic Oxygen Evolution Reaction Rates Using NiFe Oxide

Cytoprotective autophagy induction by withaferin A in prostate cancer cells involves GABARAPL1

Analysis of the function of microRNA-375 in humans using bioinformatics

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