Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis

Zhang, Xinbao; Li, Yuting; Ji, Junxiang; Wang, Xin; Zhang, Meng; Li, Xiangfen; Zhang, Yan; Zhu, Zhifeng; Ye, Shoudong; Wang, Xiaoxiao

doi:10.1038/s41420-021-00667-x

Cited by 21 publications

(13 citation statements)

References 58 publications

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“…More importantly, these four genes are closely related to cell development and differentiation. It has been reported that the expression of GADD45B is increased upon embryonic stem cells differentiation ( Zhang et al, 2021 ). Meanwhile, GADD45B is considered as a myeloid differentiation primary response gene ( Abdollahi et al, 1991 ).…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA-Seq Reveals the Cellular Diversity and Developmental Characteristics of the Retinas of an Infant and a Young Child

et al. 2022

Front. Cell Dev. Biol.

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The human retina, located in the innermost layer of the eye, plays a decisive role in visual perception. Dissecting the heterogeneity of retinal cells is essential for understanding the mechanism of visual development. Here, we performed single-cell RNA-seq to analyze 194,967 cells from the donors of infants and young children, resulting in 17 distinct clusters representing major cell types in the retina: rod photoreceptors (PRs), cone PRs, bipolar cells (BCs), horizontal cells (HCs), amacrine cells (ACs), retinal ganglion cells (RGCs), Müller glial cells (MGs), microglia, and astrocytes (ASTs). Through reclustering, we identified known subtypes of cone PRs as well as additional unreported subpopulations and corresponding markers in BCs. Additionally, we linked inherited retinal diseases (IRDs) to certain cell subtypes or subpopulations through enrichment analysis. We next constructed extensive intercellular communication networks and identified ligand-receptor interactions that play crucial roles in regulating neural cell development and immune homeostasis in the retina. Intriguingly, we found that the status and functions of PRs changed drastically between the young children and adult retina. Overall, our study offers the first retinal cell atlas in infants and young children dissecting the heterogeneity of the retina and identifying the key molecules in the developmental process, which provides an important resource that will pave the way for research on retinal development mechanisms and advancements in regenerative medicine concerning retinal biology.

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Section: Resultsmentioning

confidence: 99%

Single-Cell RNA-Seq Reveals the Cellular Diversity and Developmental Characteristics of the Retinas of an Infant and a Young Child

et al. 2022

Front. Cell Dev. Biol.

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“…A decrease in the expression level of this protein is associated with a wide variety of oncological diseases of different histogenesis. With regard to BC, there is evidence that the GADD45g protein inhibits the growth of human BC cells in vivo and in vitro, acting as an antitumour agent (Zhang et al, 2021). Moreover, the antitumour effect of the GADD45g protein can be realised independently of other important molecular factors that counteract carcinogenesis (P53, pRB, etc.…”

Section: Advances Inmentioning

confidence: 99%

Features of the Expression of Some Interferon- and Tumour-Suppressor Genes in Cats with Benign and Malignant Neoplasms of the Mammary gland

Tsybulsky¹,

Kostetsky²,

Degtyarenko³

et al. 2022

AAVS

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Background. An assessing the expression of tumour-suppressor genes is a diagnostic approach, included in the practice of veterinary oncologists. Complementing these laboratory tests with methods for assessing the expression of interferon (IFN) genes can expand the possibilities of diagnosing animal cancers and improve the accuracy of differential diagnosis of benign and malignant neoplasms, in particular of the mammary gland (MG). Methods. Surgical material obtained after unilateral mastectomy with simultaneous hysterovariectomy, as well as peripheral blood samples of cats with MG tumours was used. Blood samples and surgical material obtained from healthy cats after a planned hysterovariectomy were used as control. In cats with established diagnosis of stage II breast cancer (BC; n = 4), fibrocystic mastopathy (FCM; n = 3) and healthy cats (n = 4), the following were examined: (1) haematological parameters; (2) biochemical blood indicators; (3) expression of ifnα7, ifnβ1, ifnγ, ifnλ1, p53, rb1, cdkn2a and gadd45g genes in the BC and FCM tumor tissues, as well in the uterus tissue and WBCs using qPCR. Results. In cats with MG tumours, signs of lymphopenia and thrombocytopenia were found, these being especially pronounced in animals with BC. In cats with BC and FCM, there were significant differences in the expression patterns of the spectrum of target IFN system genes and a number of key cell cycle control factors. These differences were expressed in the tumour tissue itself, in WBCs and in the uterus tissue. Decreases of gadd45g and ifnλ1 genes expression in BC tumour tissue were the most significant. Recent findings and conclusions. The high level of p53, rb1 and cdkn2a gene expression in animals with BC can serve as a criterion for the intensity of the mechanisms of antitumour surveillance in general. However due to the inhibition of gadd45g gene expression, the described expression pattern indicates an imbalance in the molecular censor systems that protect the health of the cellular genome. Evaluation of the expression levels of this spectrum of genes, especially the gadd45g and ifnλ1 genes, may be useful as an additional criterion for the differential diagnosis of benign and malignant breast diseases in cats.

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“…Te 45G (GADD45G), a member of the GADD45 family, participates in DNA damage response and cell growth arrest. Studies have confrmed that GADD45G is lowly expressed in esophageal squamous cell carcinoma, breast cancer, and acute myeloid leukemia [14][15][16]. It has also been reported that the upregulation of GADD45G expression induces the senescence of HCC cells [17].…”

Section: Introductionmentioning

confidence: 99%

4‐Methoxydalbergione Elicits Anticancer Effects by Upregulation of GADD45G in Human Liver Cancer Cells

Zeng

Qin

et al. 2023

Journal of Healthcare Engineering

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Background. 4-Methoxydalbergione (4MOD) is a flavonoid isolated from the heartwood of Dalbergia. Studies have demonstrated that 4MOD exerts anticancer activities on bladder cancer and astrocytoma. However, the anticancer activity of 4MOD in hepatocellular carcinoma (HCC) remains unknown. This study aims to examine its anticancer activities and mechanisms in human liver cancer cells. Methods. CCK-8, colony forming, wound healing, transwell migration, and AnnexinV/PI assays were used to assess the anticancer effects of 4MOD in HCC cells. RNA sequencing (RNA-Seq) was selected to explore the possible mechanisms underlying the anti-HCC activity of 4MOD. The mRNA expression levels of target genes were verified through quantitative real-time PCR (qRT-PCR). A lentiviral shRNA interference technique was used to silence GADD45G expression. GADD45G knockdown was employed to confirm the crucial role of GADD45G in the 4MOD-mediatedanti-HCC effects. Results. 4MOD inhibited HCC cells’ proliferation and migration and promoted tumor cell apoptosis. RNA-Seq and qRT-PCR analyses revealed that 4MOD treatment increased GADD45G expression. Silencing GADD45G reversed 4MOD-mediated inhibition of proliferation, migration, and promotion of apoptosis. Conclusions. Our findings show that 4MOD elicits anti-HCC effects by upregulating GADD45G expression and could be a valuable anticancer agent for liver cancer.

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Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis

Cited by 21 publications

References 58 publications

Single-Cell RNA-Seq Reveals the Cellular Diversity and Developmental Characteristics of the Retinas of an Infant and a Young Child

Single-Cell RNA-Seq Reveals the Cellular Diversity and Developmental Characteristics of the Retinas of an Infant and a Young Child

Features of the Expression of Some Interferon- and Tumour-Suppressor Genes in Cats with Benign and Malignant Neoplasms of the Mammary gland

4‐Methoxydalbergione Elicits Anticancer Effects by Upregulation of GADD45G in Human Liver Cancer Cells

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