Gadolinium at Low Concentration Suppresses both Osteoclastic and Osteoblastic Activities in the Scales of Goldfish

Suzuki, Nobuo; Watanabe, Kouichi; Sekimoto, Aika; Urata, Makoto; Zanaty, Mohamed I.; Sekiguchi, Toshio; Kitani, Yoichiro; Matsubara, Hideaki; Srivastav, Ajai K.; Hattori, Atsuhiko

doi:10.3844/ajessp.2019.137.144

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…In addition to calcemic hormones, our bioassay was sensitive to pollutants. The concentrations of cadmium and gadolinium (even at 10 −13 M) functioned in osteoclasts in the scales of goldfish [90,91]. Also, even 10 −10 M tributyltin, a kind of marine environmental pollutant, significantly inhibited osteoblastic activity in goldfish [92].…”

Section: Toxicity Of Pahs On the Bone Metabolism Of Fishmentioning

confidence: 98%

Toxicities of Polycyclic Aromatic Hydrocarbons for Aquatic Animals

Honda

Suzuki

2020

IJERPH

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412

126

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Polycyclic aromatic hydrocarbons (PAHs) are organic compounds that are widely distributed in the air, water, and soil. Recently, the amount of PAHs derived from fuels and from incomplete combustion processes is increasing. In the aquatic environment, oil spills directly cause PAH pollution and affect marine organisms. Oil spills correlate very well with the major shipping routes. Furthermore, accidental oil spills can seriously impact the marine environment toxicologically. Here, we describe PAH toxicities and related bioaccumulation properties in aquatic animals, including invertebrates. Recent studies have revealed the toxicity of PAHs, including endocrine disruption and tissue-specific toxicity, although researchers have mainly focused on the carcinogenic toxicity of PAHs. We summarize the toxicity of PAHs regarding these aspects. Additionally, the bioaccumulation properties of PAHs for organisms, including invertebrates, are important factors when considering PAH toxicity. In this review, we describe the bioaccumulation properties of PAHs in aquatic animals. Recently, microplastics have been the most concerning environmental problem in the aquatic ecosystem, and the vector effect of microplastics for lipophilic compounds is an emerging environmental issue. Here, we describe the correlation between PAHs and microplastics. Thus, we concluded that PAHs have a toxicity for aquatic animals, indicating that we should emphasize the prevention of aquatic PAH pollution.

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Section: Toxicity Of Pahs On the Bone Metabolism Of Fishmentioning

confidence: 98%

Toxicities of Polycyclic Aromatic Hydrocarbons for Aquatic Animals

Honda

Suzuki

2020

IJERPH

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412

126

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“…Even Gd at a concentration of 10 −13 M suppressed osteoclastic activity at 6 h of incubation. Osteoblastic activity was also suppressed by Gd in the concentration range of 10 −10 to 10 −6 M at 6 h of incubation [41]. Therefore, the toxicity of Gd to osteoclasts was comparable to that of Cd.…”

Section: Discussionmentioning

confidence: 85%

“…The concentration of cadmium (even at 10 −13 M) influenced osteoclastic activity in the scale [39]. Recently, we demonstrated the toxicity of gadolinium (Gd) on osteoclasts and osteoblasts of goldfish scales [41]. Even Gd at a concentration of 10 −13 M suppressed osteoclastic activity at 6 h of incubation.…”

Section: Discussionmentioning

confidence: 99%

Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Zanaty

Sawada

Kitani

et al. 2020

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It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution.

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“…Goldfish scales are known to be highly sensitive to environmental contaminants. For example, the osteoclasts of goldfish have been found to be highly sensitive to heavy metals such as cadmium (Cd) and gadolinium (Gd) (Suzuki et al, 2004;Suzuki et al, 2019). Cd at concentrations as low as 10 −13 M suppressed TRAP activity following 6 h of incubation (Suzuki et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, fish scales are equipped with both bone-resorptive cells (osteoclasts) as well as bone-forming cells (osteoblasts) (Suzuki et al, 2008;Suzuki et al, 2016a;Hirayama et al, 2023). Given the physical properties and biological significance of fish scales, we have developed a scale-based in vitro bioassay system to evaluate seawater pollution (Suzuki et al, 2016b;Zanaty et al, 2019). In a previous study, we used this system to test seawater from the Suez Canal that was contaminated with PAHs (Suzuki et al, 2016b).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Seawater Toxicity at Five Concentrated Seawater Discharge Sites in Korea Using a Goldfish Scale-Based In Vitro Bioassay System

Chun-Sang,

Kouhei,

Masayuk

et al. 2023

Intern. J. Bio. Env. Invest.

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This study examined seawater toxicity at five concentrated seawater discharge sites in Korea using a goldfish scale-based in vitro bioassay system to examine the toxic effects of waste seawater from seawater desalination on aquatic animals. Goldfish were anesthetized and normal scales were removed to allow for scale regeneration. On day 14, the goldfish were anesthetized again, and the regenerating scales were removed. We then examined the influence of discharged seawater samples on osteoclasts and osteoblasts in regenerating scales using tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as markers of osteoclast and osteoblast. Scales were first incubated for 6 h in Leibovitz's L-15 medium supplemented with seawater samples (diluted 10 times with L-15 medium). The effect of exposure to seawater on osteoclasts and osteoblasts was then assessed and compared to surface seawater samples sourced from Tsukumo bay (Noto Peninsula, Ishikawa Prefecture, Japan) as a control. We then measured TRAP and ALP activities in all samples from five seawater discharge sites. Our results showed that the diluted seawater samples from the five discharge sites did not influence TRAP and ALP activity as compared with diluted control seawater samples. Therefore, the results of this scale-based bioassay system permit us to conclude that discharged seawater samples did not exert toxic effects under the present conditions.

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Gadolinium at Low Concentration Suppresses both Osteoclastic and Osteoblastic Activities in the Scales of Goldfish

Cited by 5 publications

References 25 publications

Toxicities of Polycyclic Aromatic Hydrocarbons for Aquatic Animals

Toxicities of Polycyclic Aromatic Hydrocarbons for Aquatic Animals

Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata

Analysis of Seawater Toxicity at Five Concentrated Seawater Discharge Sites in Korea Using a Goldfish Scale-Based In Vitro Bioassay System

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