Data from 7 mesocosms with a gradient in daily nutrient loading rate (L N ) and inverse modelling were used to estimate all C, N and P flows in an idealised food web. The gradient in L N created autotrophic production (AP) of 7 to 314 µg C l −1 d −1 (mean values over 18 d). The production of dissolved organic C (DOC) decreased from 240 to 40% of AP, and was dominated by heterotrophs (80 to 58%), with heterotrophic bacteria (BAC) producing 48 ± 8% (means ± SD) by excretion or lysis. The consumption of DOC by BAC was 133 to 86% of AP, and DOC accumulated when experimental L N ≥ natural L N . C of BAC origin constituted 5.5 to 0.2% of the C consumed by copepods. The production of dissolved N and P was mainly by heterotrophs (92 ± 2% of DN, 92 ± 4% DP), but autotrophs produced 26 ± 7% of dissolved organic N (DON) and 21 ± 8% of dissolved organic P (DOP). For the production of inorganic N and P (DIN and DIP, respectively), BAC predators produced > 50% of DIN and > 70% of DIP at low or moderate L N (averages 37 ± 16% of DIN and 66 ± 14% of DIP). The contribution by BAC was low for DIN (12 ± 6%) and zero for DIP. For the re-mineralisation of N and P, the significance of BAC was as N and P rich food particles. DOP accumulated more than DOC, despite the high demand for P. Turnover time of dissolved organic matter (DOM) decreased from around 80 to 12 d with increasing L N . These data provide new insights into the role of various functional groups of organisms for the cycling of DOM, and to the differences in C, N and P cycling.