Gains in soil C storage under anthropogenic N deposition are rapidly lost following its cessation

Abstract

Anthropogenic nitrogen (N) deposition enhanced the global terrestrial carbon (C) sink and partially offset anthropogenic CO2emissions. This was driven by the suppression of microbial activity associated with the breakdown of soil organic matter. However, since the implementation of emission abatement policies in the 1970’s, atmospheric N deposition has declined globally, and the consequences of this decline are unknown. Here, we assessed the response of soil C storage, and associated microbial activities, in a long-term field study that experimentally increased N deposition for 24-years. We measured soil C and N, parameters of microbial activity, and compared effect sizes of soil C in response to, and in recovery from, the N deposition treatment across the history of our experiment (1994-2022). Our results demonstrate that the accumulated C in the organic horizon has been lost and exhibit additional deficits 5-years post-termination of the N deposition treatment. These findings, in part, arise from mechanistic changes in microbial activity. Soil C in the mineral soil was less responsive thus far in recovery. If these organic horizon C dynamics are similar in other temperate forests, the Northern Hemisphere C sink will be reduced and climate warming will be enhanced.