Purpose: To quantify gait asymmetry in well-trained runners with and without previous injuries during interval training sessions incorporating different distances. Methods: Twelve well-trained runners participated in 8 high-intensity interval-training sessions on a synthetic track over a 4-wk period. The training consisted of 10 × 400, 8 × 600, 7 × 800, and 6 × 1000-m running. Using an inertial measurement unit, the ground-contact time (GCT) of every step was recorded. To determine gait asymmetry, the GCTs between the left and right foot were compared. Results: Overall, gait asymmetry was 3.3% ± 1.4%, and over the course of a training session, the gait asymmetry did not change (F 1,33 = 1.673, P = .205). The gait asymmetry of the athletes with a previous history of injury was significantly greater than that of the athletes without a previous injury. However, this injuryrelated enlarged asymmetry was detectable only at short (400 m), but not at longer, distances (600-1000 m). Conclusion: The gait asymmetry of well-trained athletes differed, depending on their history of injury and the running distance. To detect gait asymmetries, high-intensity runs over relatively short distances are recommended.Keywords: imbalance, field condition, fatigue, inertial measurement unit, high performance Athletes and coaches assume that gait asymmetry affects athletic performance, as 1 of the lower limbs is exposed to more stress than the other. 1,2 Gait asymmetry can be the primary cause of an injury and it can be responsible for an injury. 1,3,4 Previously, gait asymmetries ranging from 3% for knee angle at touchdown to 54% for hip-angle velocity were reported in female runners, 5,6 suggesting that a single threshold level of normal to problematic gait asymmetry does not exist and that there are wide variations in gait mechanics and asymmetries. 1,18 Also, gait asymmetry may not be evident at the beginning of a race but may emerge with fatigue. Regarding injury history, some earlier studies of athletes with and without a previous injury found no differences in gait asymmetry. 1,7,8 However, all the subjects in the aforementioned studies were either novice runners, 7 the assessments took place in a laboratory setting (on a treadmill) at submaximal speeds only, 1,7-9 and only 1 trial 10 or a few gait cycles 9 were analyzed. Consequently, it is unclear whether these results are congruent with real practice during training or competition on realistic floor conditions. For instance, a previous study provided evidence that gait asymmetry is reduced on a treadmill. 11 Hence, to better understand the occurrence of gait asymmetry, data from well-trained runners over entire runs over various distances, at high speeds, and at high intensities need to be obtained in field measurements.The purpose of this study therefore was threefold: to quantify gait asymmetry in healthy well-trained runners during high-intensity track runs of different lengths and speeds (400-1000 m), to evaluate the effect of an injury in the previous 24 months on gait as...