Postural inaccuracies in persistent dental tasks indicated an upsurge in the prevalence of musculoskeletal disorders in dentists. This makes it imperative to restrain awkward postural movements while working. Biased results in self-reporting surveys; discomfort, expense, and time consumption involved in using wearable sensors; and expert’s opinion are required in observational methods. Hence, it is important to use significantly reliable, cheap technology as a substitute to overcome the shortcomings of the mentioned techniques. In this study, the markerless Kinect V2–based system was developed and compared with the conventional imaging technique for real-time postural assessment of dental seating tasks. The study assessed the angle parameters related to the dentist’s bodily movement of upper arm, lower arm, wrist, neck, and trunk. Ten dentists from the local dental institution volunteered for the study. Dentists were monitored with both techniques while performing real-time dental procedures. The agreement between the techniques was assessed using Bland–Altman plot at 95% bias, Pearson’s (r1) and concordance (r2) correlation coefficients, mean difference, and percentage error. For conclusive agreement analysis, contingency coefficient, proportion agreement index, Cohen’s kappa, and Mann–Whitney U at 95% confidence interval (CI) were evaluated. Data acquired from both techniques possessed strong correlations (r1 and r2 >0.90). Good agreement in Rapid Upper Limb Assessment data using Cohen’s kappa (0.67) at standard Landis and Koch’s scale was also observed. Postural analysis of slow-motion tasks like dentistry using the Kinect V2 system proved to be unobtrusive and efficient. This may be used by dentists to have periodic postural check. In future, Kinect V2–based feedback system may be used to develop an assistive technology using predictive algorithms, which may help in reducing the probability of occurrence of work-related musculoskeletal disorders in dentists.