Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p

Lim, Mei Kee; Tang, Vivien Chia Yen; Saux, Agnès Le; Schüller, Jutta; Bongards, Christine; Lehming, Norbert

doi:10.1016/j.jmb.2007.09.013

Cited by 21 publications

(21 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We utilized the Split Ubiquitin assay to detect interactions between Gal11 and ScMSN2, ScMsn2-3DA, CgMSN2 or CgMsn2-3DA. In this assay, the interaction between Gal11 and Msn2 leads to the assembly of two halves of ubiquitin, each fused to the interacting proteins, that in turn leads to the degradation of the fused Ura3 protein (Lim et al, 2007). We found that both ScMsn2 and CgMsn2 can bind to Gal11, leading to a lower growth level of the respective strains, relative to the control strains on uracil-lacking plates (Fig.…”

Section: Msn2 Motif a And Motif B Are Essential For Msn2 And Gal11 Inmentioning

confidence: 74%

“…The split ubiquitin is a Y2H assay that is based on conditional proteolysis of ubiquitinated Ura3-conjugated proteins (Snider et al, 2010). This assay was extensively used to monitor interactions between membrane proteins and between transcriptional-activating domains, such as Gal4 and Gcn4, with other cellular proteins (Lim et al, 2007;Snider et al, 2010). We utilized the Split Ubiquitin assay to detect interactions between Gal11 and ScMSN2, ScMsn2-3DA, CgMSN2 or CgMsn2-3DA.…”

Section: Msn2 Motif a And Motif B Are Essential For Msn2 And Gal11 Inmentioning

confidence: 99%

See 1 more Smart Citation

Conserved Motifs in the Msn2-Activating Domain are Important for Msn2-Mediated Yeast Stress Response

Sadeh

Baran

Volokh

et al. 2012

Journal of Cell Science

View full text Add to dashboard Cite

SummaryThe Msn2 and Msn4 transcription factors play crucial roles in the yeast general stress response. Previous studies identified several large functional domains of Msn2, mainly through crude truncations. Here, using bioinformatics and experimental approaches to examine Msn2 structure-function relationships, we have identified new functional motifs in the Msn2 transcriptional-activating domain (TAD). Msn2 is predicted to adopt an intrinsically disordered structure with two short structural motifs in its TAD. Mutations in these motifs dramatically decreased Msn2 transcriptional activity, yeast stress survival and Msn2 nuclear localization levels. Using the split-ubiquitin assay, we found that these motifs are important for the interaction of Msn2 with Gal11, a subunit of the mediator complex. Finally, we show that one of these motifs is functionally conserved in several yeast species, highlighting a common mechanism of Msn2 transcriptional activation throughout yeast evolution.

show abstract

Section: Msn2 Motif a And Motif B Are Essential For Msn2 And Gal11 Inmentioning

confidence: 74%

Section: Msn2 Motif a And Motif B Are Essential For Msn2 And Gal11 Inmentioning

confidence: 99%

Conserved Motifs in the Msn2-Activating Domain are Important for Msn2-Mediated Yeast Stress Response

Sadeh

Baran

Volokh

et al. 2012

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…TFIID induces DNA bending that may facilitate interactions between regulatory factors and promoters of their targets (52). Considering that Taf13p and Gcn4p physically interact (40), polymorphism within TAF13 plausibly affects the efficiency of transcription of Gcn4p target genes. Mbf1p is a coactivator of Gcn4p-dependent transcription (24).…”

Section: Discussionmentioning

confidence: 99%

“…6). Taf13p, a TATA binding protein-associated factor, is a subunit of TFIID that interacts with Gcn4p in vivo (40). We identified three nonsynonymous SNPs within the coding region of TAF13 between RM and S288c, a strain similar to BY: A137G, A138V, and G158A.…”

Section: Searching For Genetic Modulators Of Gcn4p-mediated Amino Acidmentioning

confidence: 98%

“…We also identified a region on chromosome XV that contains 67 genes including SNF2. This gene encodes the catalytic subunit of the SWI/SNF chromatin remodeling complex (41,42) and interacts physically with Gcn4p (40,43,44). There are nine amino acid polymorphisms in Snf2p between the RM and S288c strains.…”

Section: Searching For Genetic Modulators Of Gcn4p-mediated Amino Acidmentioning

confidence: 99%

See 1 more Smart Citation

Identifying genetic modulators of the connectivity between transcription factors and their transcriptional targets

Fazlollahi

Muroff

Lee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Regulation of gene expression by transcription factors (TFs) is highly dependent on genetic background and interactions with cofactors. Identifying specific context factors is a major challenge that requires new approaches. Here we show that exploiting natural variation is a potent strategy for probing functional interactions within gene regulatory networks. We developed an algorithm to identify genetic polymorphisms that modulate the regulatory connectivity between specific transcription factors and their target genes in vivo. As a proof of principle, we mapped connectivity quantitative trait loci (cQTLs) using parallel genotype and gene expression data for segregants from a cross between two strains of the yeast Saccharomyces cerevisiae. We identified a nonsynonymous mutation in the DIG2 gene as a cQTL for the transcription factor Ste12p and confirmed this prediction empirically. We also identified three polymorphisms in TAF13 as putative modulators of regulation by Gcn4p. Our method has potential for revealing how genetic differences among individuals influence gene regulatory networks in any organism for which gene expression and genotype data are available along with information on binding preferences for transcription factors.

show abstract

Chromatin regulator Ahc1p co-regulates nitrogen metabolism via interactions with multiple transcription factors in Saccharomyces cerevisiae

Chen

Zeng

et al. 2023

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p

Cited by 21 publications

References 71 publications

Conserved Motifs in the Msn2-Activating Domain are Important for Msn2-Mediated Yeast Stress Response

Conserved Motifs in the Msn2-Activating Domain are Important for Msn2-Mediated Yeast Stress Response

Identifying genetic modulators of the connectivity between transcription factors and their transcriptional targets

Chromatin regulator Ahc1p co-regulates nitrogen metabolism via interactions with multiple transcription factors in Saccharomyces cerevisiae

Contact Info

Product

Resources

About