Galacto-oligosaccharides may directly enhance intestinal barrier function through the modulation of goblet cells

Bhatia, Shikha; Prabhu, Ponnuraj Nagendra; Benefiel, Ann C.; Miller, Michael J.; Chow, John W.; Davis, Steven R.; Gaskins, H. Rex

doi:10.1002/mnfr.201400639

Cited by 113 publications

(96 citation statements)

References 49 publications

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“…This mechanism may be important in reducing the extent of gastric mucosal damage caused by the administration of NSAIDs [89]. In addition, after treating with prebiotic galacto-oligosaccharides (GOS), the expressions of MUC2 and TFF3 are significantly up-regulated (Table 2), which indicates that GOS may enhance mucosal protective function via direct stimulation of intestinal goblet cells [90].…”

Section: Gastrin Nsaids and Gos Upregulate The Expression Of Tff2/ Tff3mentioning

confidence: 98%

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Xiao

Ling

Lan

et al. 2015

Clinica Chimica Acta

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Section: Gastrin Nsaids and Gos Upregulate The Expression Of Tff2/ Tff3mentioning

confidence: 98%

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Xiao

Ling

Lan

et al. 2015

Clinica Chimica Acta

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“…GOS also has effects on intestinal epithelial cells and has been reported to stimulate gut barrier function (Bhatia et al 2015). An in vitro study by Bhatia et al (Bhatia et al 2015) showed that GOS enhanced intestinal barrier function through modulation of the secretory function of human goblet cells by up regulation of related genes and protein expression (Bhatia et al 2015).…”

Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

“…An in vitro study by Bhatia et al (Bhatia et al 2015) showed that GOS enhanced intestinal barrier function through modulation of the secretory function of human goblet cells by up regulation of related genes and protein expression (Bhatia et al 2015). GOS can also stimulate in vitro tight junction assembly in gut intestinal human Caco-2 cells, which is beneficial for the integrity of the epithelial barrier (Akbari et al 2015).…”

Section: Effects Of Gos On Epithelial Cells Responsesmentioning

confidence: 99%

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Akkerman

Faas²,

Vos

2018

Critical Reviews in Food Science and Nutrition

139

140

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Human milk (HM) is the golden standard for nutrition of newborn infants. Human milk oligosaccharides (HMOs) are abundantly present in HM and exert multiple beneficial functions, such as support of colonization of the gut microbiota, reduction of pathogenic infections and support of immune development. HMO-composition is during lactation continuously adapted by the mother to accommodate the needs of the neonate. Unfortunately, for many valid reasons not all neonates can be fed with HM and are either totally or partly fed with cow-milk derived infant formulas, which do not contain HMOs. These cow-milk formulas are supplemented with non-digestible carbohydrates (NDCs) that have functional effects similar to that of some HMOs, since production of synthetic HMOs is challenging and still very expensive. However, NDCs cannot substitute all HMO functions. More efficacious NDCs may be developed and customized for specific groups of neonates such as pre-matures and allergy prone infants. Here current knowledge of HMO functions in the neonate in view of possible replacement of HMOs by NDCs in infant formulas is reviewed. Furthermore, methods to expedite identification of suitable NDCs and structure/function relationships are reviewed as in vivo studies in babies are impossible.

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“…Effects of hMOSs were compared to effects of GOS, which is currently being applied in infant formula as a substitute for hMOs and known to enhance intestinal barrier function through the modulation of goblet cells . We examined gene expression alterations of the goblet cell secretory related genes MUC2 , TFF3 , and RETNLB , and the Golgi‐sulfotransferase genes CHST5 and GAL3ST2 in the human goblet cell line LS174T .…”

Section: Introductionmentioning

confidence: 99%

Human Milk Oligosaccharides Differently Modulate Goblet Cells Under Homeostatic, Proinflammatory Conditions and ER Stress

Cheng

Kong

Walvoort

et al. 2019

Molecular Nutrition Food Res

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Scope Human milk oligosaccharides (hMOs) have beneficial effects on intestinal barrier function, but the mechanisms of action are not well understood. Here, the effects of hMOs on goblet cells, which indicate that some hMOs may enhance mucus barrier function through direct modulation of goblet cell function, are studied. Methods and results The modulatory effects of 2′‐fucosyllactose (2′‐FL), 3‐fucosyllactose (3‐FL), lacto‐N‐triaose II (LNT2), and galacto‐oligosaccharides (GOS) on the expression of goblet cell secretory related genes MUC2, TFF3, and RETNLB, and the Golgi‐sulfotransferase genes CHST5 and GAL3ST2 of LS174T are determined by real‐time quantitative RT‐PCR. 3‐FL, LNT2, and GOS‐modulated LS174T gene expression profiles in a dose‐ and time‐dependent manner. In addition, the upregulation of MUC2 is confirmed by immunofluorescence staining. Effects of 2′‐FL, 3‐FL, LNT2, and GOS on gene transcription of LS174T are also assessed during exposure to TNF‐α, IL‐13, or tunicamycin. During TNF‐α challenge, 3‐FL and LNT2 enhance MUC2 and TFF3 gene expression. After IL‐13 exposure, 2′‐FL, 3‐FL, and LNT2 all show upregulating effects on MUC2; 3‐FL and LNT2 also enhance TFF3 expression. LNT2 significantly reverses Tm‐induced downregulation of TFF3, RETNLB, and CHST5. Conclusion The findings indicate that hMOs may enhance mucus barrier function through direct modulation of intestinal goblet cells. Effects are structure‐ and stressor‐dependent.

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Galacto-oligosaccharides may directly enhance intestinal barrier function through the modulation of goblet cells

Abstract: Collectively, the data indicate that GOS may enhance mucosal barrier function through direct stimulation of intestinal goblet cells.

Cited by 113 publications

References 49 publications

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Non-digestible carbohydrates in infant formula as substitution for human milk oligosaccharide functions: Effects on microbiota and gut maturation

Human Milk Oligosaccharides Differently Modulate Goblet Cells Under Homeostatic, Proinflammatory Conditions and ER Stress

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