Galactoxyloglucan-doxorubicin nanoparticles exerts superior cytotoxic effects on cancer cells⿿A mechanistic and in silico approach

Joseph, Manu M.; Aswathy, G; Manojkumar, T.K.; Sreelekha, T.T.

doi:10.1016/j.ijbiomac.2016.06.093

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…This macrophage polarization effect will in turn promote the cell mediated antitumor immune response. The execution of programmed cell death in a caspase-dependent fashion by the PST001 and its various NPs was previously established by multiple studies. ,, A large variety of CuNPs fabricated using different approaches are reported to cause cell death through the execution of apoptosis. , Lack of any signs of toxicity by our NPs was established with the cell cycle analysis, which revealed the inability of CuNP@PST to alter the cell cycle phases. A stimulatory effect on wound closure with the normal keratinocytes indicated the proliferative potential of CuNP@PST on normal cells.…”

Section: Results and Discussionmentioning

confidence: 74%

Galactoxyloglucan Endowed Biogenic Nanoimmunobiotics Arrests Microbial Growth and Elicits Antitumor Immunity

Joseph

Hari

Pillai

et al. 2019

ACS Appl. Bio Mater.

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A straightforward investigation with a surfactant-free green method mediated orchestration of a nanocomposite, where immunotherapy was fused with antimicrobial ability in a biogenic amalgamation, resulted in a nanoimmunobiotics (NIB). Consequently, a galactoxyloglucan (PST001) isolated from Tamarindus indica seed kernel was utilized in a sustainable approach to endow copper nanoparticles (CuNP@PST) with high stability and broad pH tolerance. The biocompatible nature of the CuNP@PST was demonstrated by toxicity studies on BALB/c mice. The nanoparticles exhibited immunomodulatory features by stimulating both humoral and cell-mediated immunity. As remarkable aftermath, we observed promising wound healing capacity on normal human keratinocytes along with the excellent antibacterial and antifungal activity by the NIB. The unique potential of CuNP@PST as a chemopreventive agent by eliciting antitumor immunity was illustrated on syngeneic murine tumor models. The outstanding capacity to reduce tumor burden with a significant increase in lifespan and a peculiar cytokine activation pattern could materialize these nanoparticles as an adjuvant for cancer management. The intrinsically bioeliminable, well-tolerated, and systemically available NIB promises to enter clinical testing as an antimicrobial-immunotherapy agent. Appreciable biodistribution pattern, along with the cost-effective fabrication strategies for scale-up, presents a futuristic biomedical scenario for fast promoting the translational potential of our NIB to combat antibiotic resistance and neoplasia effectively.

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Section: Results and Discussionmentioning

confidence: 74%

Galactoxyloglucan Endowed Biogenic Nanoimmunobiotics Arrests Microbial Growth and Elicits Antitumor Immunity

Joseph

Hari

Pillai

et al. 2019

ACS Appl. Bio Mater.

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“…Furthermore, evaluation of apoptosis by FITC-Annexin V staining (BD Pharmingen no. 556547, BD Biosciences, San Jose, CA) was also performed by flow cytometry, using kit-specified instructions on a FACS Calibur flow cytometer (BD Biosciences, San Jose, CA), and the data were analyzed with the Cell QuestPro software . Early onset of programmed cell death was further observed using APOPercentage dye (Biocolor, Belfast, Northern Ireland) as per manufacturer’s instructions as previously described .…”

Section: Methodsmentioning

confidence: 99%

“…556547, BD Biosciences, San Jose, CA) was also performed by flow cytometry, using kit-specified instructions on a FACS Calibur flow cytometer (BD Biosciences, San Jose, CA), and the data were analyzed with the Cell QuestPro software. 25 Early onset of programmed cell death was further observed using APOPercentage dye (Biocolor, Belfast, Northern Ireland) as per manufacturer's instructions as previously described. 26 Light microscopic images of APOPercentage dye-labeled cells, which stained pink under a light microscope, were used to quantify the extent of apoptosis.…”

Section: Splenocyte Proliferation Assaymentioning

confidence: 99%

Plasmonically Enhanced Galactoxyloglucan Endowed Gold Nanoparticles Exposed Tumor Targeting Biodistribution Envisaged in a Surface-Enhanced Raman Scattering Platform

et al. 2017

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Biopolymer-capped gold nanoparticles (AuNPs) were perceived for tracing biodistribution in a solid tumor mice through surface-enhanced Raman scattering (SERS) fingerprinting. In this strategy, a robust and ecofriendly green chemistry approach was adopted to construct galactoxyloglucan (PST001) endowed AuNPs (PST-GNPs) with cancer-cell-selective toxic nature and excellent biocompatibility. Plasmonically enhanced light-scattering properties facilitated PST-GNPs to be a superior SERS substrate with high Raman signal enhancement. In this context, PST-GNPs were scrutinized for the noninvasive label-free SERS live-cell spectral imaging to evaluate the fingerprint molecular details of cellular processes. Consequently, the inherent SERS feature of PST-GNPs enabled us to investigate the dynamic and complex nature with NP biodistrubution in tumor-bearing mice on a SERS platform that illustrated the tumor targeting nature. Henceforth, the present findings emphasized a futuristic clinically relevant scenario for tracing the in vivo NP dissemination in a label-free fashion for providing vital biochemical details on a molecular level.

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“…As a type of anthracycline antibiotic, DOX is a good iron chelator and the chelate formation can be used in drug delivery systems. 35 By incubating MTB with DOX in the growth medium, the complexation between ferrous iron and DOX in the vesicles was obtained and DOX-Fe chelate-loaded MVs (BMV@DOX) were isolated. Furthermore, to endow the vesicles with tumor specificity and alleviate the systemic toxicity, BMV@DOX was functionalized with DSPE-PEG 5000 -cRGD peptides to form a T-BMV@DOX nanovaccine with tumor-targeting capacity (Scheme 1a).…”

Section: Introductionmentioning

confidence: 99%

Engineering magnetotactic bacteria MVs to synergize chemotherapy, ferroptosis and immunotherapy for augmented antitumor therapy

2023

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Galactoxyloglucan-doxorubicin nanoparticles exerts superior cytotoxic effects on cancer cells⿿A mechanistic and in silico approach

Cited by 7 publications

References 23 publications

Galactoxyloglucan Endowed Biogenic Nanoimmunobiotics Arrests Microbial Growth and Elicits Antitumor Immunity

Galactoxyloglucan Endowed Biogenic Nanoimmunobiotics Arrests Microbial Growth and Elicits Antitumor Immunity

Plasmonically Enhanced Galactoxyloglucan Endowed Gold Nanoparticles Exposed Tumor Targeting Biodistribution Envisaged in a Surface-Enhanced Raman Scattering Platform

Engineering magnetotactic bacteria MVs to synergize chemotherapy, ferroptosis and immunotherapy for augmented antitumor therapy

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