Galangin (3,5,7-Trihydroxyflavone) Shields Human Keratinocytes from Ultraviolet B-Induced Oxidative Stress

Hewage, Susara Ruwan Kumara Madduma; Piao, Mei Jing; Kim, Ki Cheon; Won, Ji; Han, Xiaodong; Choi, Yung Hyun; Chae, Sungwook; Hyun, Jin Won

doi:10.4062/biomolther.2014.130

Cited by 24 publications

(11 citation statements)

References 50 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, we demonstrated that galangin attenuates oxidative DNA damage, lipid and protein oxidation, and expression of apoptotic proteins in human keratinocytes under UVB-induced oxidative stress (Madduma Hewage et al ., 2015). It remained unclear, however, how galangin interacts with cellular antioxidant defense mechanisms and augments cellular defenses.…”

Section: Introductionmentioning

confidence: 95%

“…Galangin (3,5,7-trihydroxyflavone), a flavonoid abundant in Alpinia officinarum and Helichrysum aureonitens ( Itoh et al ., 1999 ; Ciolino and Yeh, 1999 ), has antibacterial, antiviral, and anticancer properties ( Afolayan and Meyer, 1997 ; Cushnie and Lamb, 2006 ). In a previous study, we demonstrated that galangin attenuates oxidative DNA damage, lipid and protein oxidation, and expression of apoptotic proteins in human keratinocytes under UVB-induced oxidative stress ( Madduma Hewage et al ., 2015 ). It remained unclear, however, how galangin interacts with cellular antioxidant defense mechanisms and augments cellular defenses.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Galangin Activates the ERK/AKT-Driven Nrf2 Signaling Pathway to Increase the Level of Reduced Glutathione in Human Keratinocytes

Hewage

Piao

Kang

et al. 2017

Biomolecules & Therapeutics

Self Cite

View full text Add to dashboard Cite

Previously, we demonstrated that galangin (3,5,7-trihydroxyflavone) protects human keratinocytes against ultraviolet B (UVB)-induced oxidative damage. In this study, we investigated the effect of galangin on induction of antioxidant enzymes involved in synthesis of reduced glutathione (GSH), and investigated the associated upstream signaling cascades. By activating nuclear factor-erythroid 2-related factor (Nrf2), galangin treatment significantly increased expression of glutamate-cysteine ligase catalytic subunit (GCLC) and glutathione synthetase (GSS). This activation of Nrf2 depended on extracellular signal-regulated kinases (ERKs) and protein kinase B (AKT) signaling. Inhibition of GSH in galangin-treated cells attenuated the protective effect of galangin against the deleterious effects of UVB. Our results reveal that galangin protects human keratinocytes by activating ERK/AKT-Nrf2, leading to elevated expression of GSH-synthesizing enzymes.

show abstract

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 95%

Galangin Activates the ERK/AKT-Driven Nrf2 Signaling Pathway to Increase the Level of Reduced Glutathione in Human Keratinocytes

Hewage

Piao

Kang

et al. 2017

Biomolecules & Therapeutics

Self Cite

View full text Add to dashboard Cite

show abstract

“…UV radiation is known as the primary extrinsic factor which causes skin aging (also referred to as photoaging) because of its ability to induce oxidative stress and DNA damage (Choi et al 2015). Although the skin is equipped with a complex enzymatic and nonenzymatic defense system to maintain its intracellular redox balance, prolonged UV exposure can disrupt this system (Hewage et al 2015). Particularly, UVB can damage the epidermis and result in extremely rapid photoaging (Ichihashi et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…UV irradiation is the primary extrinsic factor responsible for skin aging. UV radiation stimulates excessive reactive oxygen species (ROS) generation, which overwhelms cellular antioxidant defense systems and leads to oxidative stress in the skin (Hewage et al 2015;Ding et al 2015). These defense systems include protective enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as non-enzymatic mechanisms (Piao et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Protective skin aging effects of cherry blossom extract (Prunus Yedoensis) on oxidative stress and apoptosis in UVB-irradiated HaCaT cells

Wang¹,

Xu³

et al. 2019

Cytotechnology

View full text Add to dashboard Cite

Extracts of the cherry blossom plant have been reported to exert various biological effects on human cells. However, no previous investigations have examined the antioxidant and anti-apoptotic effects of these extracts on ultraviolet B (UVB) radiation-induced skin aging. This study explores the underlying mechanisms of the antioxidant and antiapoptotic effects of cherry blossom extract (CBE) in human keratinocyte (HaCaT) cells. HaCaT cells were treated with CBE at concentrations of 0.5, 1.0, and 2.0% for 24 h and then irradiated with UVB (40 mJ/ cm 2). CBE effectively and dose-dependently decreased the levels of reactive oxygen species and malondialdehyde, while increasing the activities of superoxide dismutase and glutathione peroxidase. Pretreatment with 1 and 2% CBE attenuated UVBinduced DNA damage by reducing the formation of cyclobutane pyrimidine dimers and 8-hydroxy-20deoxyguanosine. Furthermore, CBE also prevented UVB-induced apoptosis and significantly downregulated B cell lymphoma 2 (Bcl-2)-associated X, cytochrome-c, and caspase-3 expression, while upregulating Bcl-2 expression. Taken together, these results indicate that CBE protects HaCaT cells from UVBinduced oxidative stress and apoptosis and suggest that CBE could be a potent antioxidant against skin aging.

show abstract

“…[2][3][4] However, it also has antioxidative properties, which are attributable for its beneficial effects.…”

mentioning

confidence: 99%

Evaluation of Antimelanogenic Activity and Mechanism of Galangin <i>in Silico</i> and <i>in Vivo</i>

Chung

Jeong

Lee

et al. 2018

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Abnormal pigmentation owing to excessive melanin synthesis can result in serious problems such as freckles, age-spots, and melanoma. Tyrosinase inhibitors have been an interesting target for the treatment of hyperpigmentation because tyrosinase is the rate-limiting enzyme in melanin synthesis. The screening for strong tyrosinase inhibitors led to the finding of the flavonoid galangin, which showed notable inhibitory effects on mushroom tyrosinase. The IC 50 value of galangin (3.55 0.39 µM) was lower than that of kojic acid (48.55 1.79 µM), which was used as a positive control. In silico docking simulation and mechanistic studies demonstrated that galangin interacted with the catalytic sites of tyrosinase and competed with tyrosine. In B16F10 melanoma cells stimulated with α-melanocyte stimulating hormone, galangin inhibited tyrosinase activity as well as melanin production. Although high doses of galangin were cytotoxic, no cytotoxic effects were observed at low doses. In addition, the in vivo efficacy of galangin was evaluated in HRM2 melaninpossessing hairless mice. As measured by the skin-whitening index and melanin staining, repeated UVB exposure increased skin melanin synthesis. Galangin application significantly reduced melanogenesis induced by UVB exposure. Collectively, our data indicates that galangin shows strong tyrosinase inhibition activity, which suggests that it may be an effective skin-whitening agent.Key words galangin; melanogenesis; pigmentation; tyrosinase Galangin, a flavonoid compound, is found in high concentrations in Alpinia officinarum and Helichrysum aureonitens.

show abstract

Galangin (3,5,7-Trihydroxyflavone) Shields Human Keratinocytes from Ultraviolet B-Induced Oxidative Stress

Cited by 24 publications

References 50 publications

Galangin Activates the ERK/AKT-Driven Nrf2 Signaling Pathway to Increase the Level of Reduced Glutathione in Human Keratinocytes

Galangin Activates the ERK/AKT-Driven Nrf2 Signaling Pathway to Increase the Level of Reduced Glutathione in Human Keratinocytes

Protective skin aging effects of cherry blossom extract (Prunus Yedoensis) on oxidative stress and apoptosis in UVB-irradiated HaCaT cells

Evaluation of Antimelanogenic Activity and Mechanism of Galangin <i>in Silico</i> and <i>in Vivo</i>

Contact Info

Product

Resources

About