Galangin promotes cell apoptosis through suppression of <i>H19</i> expression in hepatocellular carcinoma cells

Zhong, Xiaowei; Huang, Siyi; Liu, Dianfeng; Jiang, Ziping; Jin, Qinglong; Li, Chengshun; Liu, Da; Yao, Qunyan; Wang, Dongxu

doi:10.1002/cam4.3195

Cited by 33 publications

(29 citation statements)

References 41 publications

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“…In recent years, natural compounds were widely used in many fields including clinical treatment due to their safety and effectiveness. Many preclinical and clinical studies have confirmed that natural compounds have certain therapeutic effects in various diseases, especially cancers (12,32,37,38). Baicalein purified from Scutellaria baicalensis Georgi is an active flavonoid ingredient, which was reported participating in inhibiting the progression of various cancers (13)(14)(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

“…Different miRNA analysis was analyzed by DESeq2. The target gene of miRNAs was derived from miRTarBase (http://mirtarbase.mbc.nctu.edu.tw/index.html) (32) and the network diagram of miRNA and target gene was drawn by Cytoscape. R-Package clusterProfiler (33) was used to analyze the target genes of miRNAs for KEGG and GO enrichment analysis.…”

Section: Construction Of Mirnas Libraries and Mirnas Expression Analysismentioning

confidence: 99%

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Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

Chen

Wang

et al. 2021

Front. Oncol.

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Pancreatic cancer is a common malignant tumor with a high incidence and mortality rate. The prognosis of patients with pancreatic cancer is considerably poor due to the lack of effective treatment in clinically. Despite numerous studies have revealed that baicalein, a natural product, is responsible for suppressing multiple cancer cells proliferation, motility and invasion. The mechanism by which baicalein restraining pancreatic cancer progression remains unclear. In this study, we firstly verified that baicalein plays a critical role in inhibiting pancreatic tumorigenesis in vitro and in vivo. Then we analyzed the alteration of microRNAs (miRNAs) expression levels in Panc-1 cells incubated with DMSO, 50 and 100 μM baicalein by High-Throughput sequencing. Intriguingly, we observed that 20 and 39 miRNAs were accordingly up- and down-regulated through comparing Panc-1 cells exposed to 100 μM baicalein with the control group. Quantitative PCR analysis confirmed that miR-139-3p was the most up-regulated miRNA after baicalein treatment, while miR-196b-5p was the most down-regulated miRNA. Further studies showed that miR-139-3p induced, miR-196b-5p inhibited the apoptosis of Panc-1 cells via targeting NOB1 and ING5 respectively. In conclusion, we demonstrated that baicalein is a potent inhibitor against pancreatic cancer by modulating the expression of miR-139-3p or miR-196b-5p.

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Section: Discussionmentioning

confidence: 99%

Section: Construction Of Mirnas Libraries and Mirnas Expression Analysismentioning

confidence: 99%

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

Chen

Wang

et al. 2021

Front. Oncol.

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“…Recently, lncRNAs and miRNAs have been demonstrated to have roles in development of different types of cancer, including HCC ( 18 ). There is evidence that reduced expression of lncRNA H19 leads to inhibition of tumor growth in breast cancer, bladder cancer and colorectal cancer ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have suggested that H19 expression suppressed endogenous let-7 while H19 mutant was not related to let-7 ( 17 ). Additionally, reduced expression of H19 induced cellular apoptosis and inhibited cell growth in HCC ( 18 ). However, there is little evidence to suggest that COPB2 expression is associated with lncRNAs and miRNAs in GC.…”

Section: Introductionmentioning

confidence: 99%

Chrysin Induced Cell Apoptosis Through H19/let-7a/COPB2 Axis in Gastric Cancer Cells and Inhibited Tumor Growth

Chen

Jiang

et al. 2021

Front. Oncol.

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BackgroundChrysin is a natural flavone that is present in honey and has exhibited anti-tumor properties. It has been widely studied as a therapeutic agent for the treatment of various types of cancers. The objectives of this present study were to elucidate how chrysin regulates non-coding RNA expression to exert anti-tumor effects in gastric cancer cells.MethodsThrough the use of RNA sequencing, we investigated the differential expression of mRNAs in gastric cancer cells treated with chrysin. Furthermore, COPB2, H19 and let-7a overexpression and knockdown were conducted. Other features, including cell growth, apoptosis, migration and invasion, were also analyzed. Knockout of the COPB2 gene was generated using the CRISPR/Cas9 system for tumor growth analysis in vivo.ResultsOur results identified COPB2 as a differentially expressed mRNA that is down-regulated following treatment with chrysin. Moreover, the results showed that chrysin can induce cellular apoptosis and inhibit cell migration and invasion. To further determine the underlying mechanism of COPB2 expression, we investigated the expression of the long non-coding RNA (lncRNA) H19 and microRNA let-7a. Our results showed that treatment with chrysin significantly increased let-7a expression and reduced the expression of H19 and COPB2. In addition, our results demonstrated that reduced expression of COPB2 markedly promotes cell apoptosis. Finally, in vivo data suggested that COPB2 expression is related to tumor growth.ConclusionsThis study suggests that chrysin exhibited anti-tumor effects through a H19/let-7a/COPB2 axis.

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“…Besides, galangin inhibits epithelial-mesenchymal transition and angiogenesis of glioma by downregulating CD44 (Chen et al 2019a ). Simultaneously, galangin promotes apoptosis of diverse cancer cells, such as hepatocellular carcinoma cells, ovarian cancer cells and glioblastoma cells (Kong et al 2019 ; Fang et al 2019 ; Zhong et al 2020 ; Huang et al 2020 ). One study suggested that galangin, either alone or combined with insulin, can reduce glucose levels and improve skeletal muscle health in patients with diabetes (Kalhotra and Chittepu 2019 ).…”

Section: Trpcs Antagonistsmentioning

confidence: 99%

Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

et al. 2021

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Canonical transient receptor potential channels (TRPCs) are nonselective, high calcium permeability cationic channels. The TRPCs family includes TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7. These channels are widely expressed in the cardiovascular and nervous systems and exist in many other human tissues and cell types, playing several crucial roles in the human physiological and pathological processes. Hence, the emergence of TRPCs modulators can help investigate these channels’ applications in health and disease. It is worth noting that the TRPCs subfamilies have structural and functional similarities, which presents a significant difficulty in screening and discovering of TRPCs modulators. In the past few years, only a limited number of selective modulators of TRPCs were detected; thus, additional research on more potent and more selective TRPCs modulators is needed. The present review focuses on the striking desired therapeutic effects of TRPCs modulators, which provides intel on the structural modification of TRPCs modulators and further pharmacological research. Importantly, TRPCs modulators can significantly facilitate future studies of TRPCs and TRPCs related diseases.

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Galangin promotes cell apoptosis through suppression of H19 expression in hepatocellular carcinoma cells

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 33 publications

References 41 publications

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

Chrysin Induced Cell Apoptosis Through H19/let-7a/COPB2 Axis in Gastric Cancer Cells and Inhibited Tumor Growth

Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

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