Galanin activated Gi/o-proteins in human and rat central nervous systems

Barreda-Gómez, Gabriel; Pazos, Ángel; Rodrı́guez-Puertas, Rafael

doi:10.1016/j.npep.2014.06.002

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…1d-f). These observations were largely 12 consistent with previous reports [16,17,28]. We then determined the cell-type distribution of GalR1 mRNA in the IL cortex and vCA1/vSub since both areas showed the highest expression of GalR1 mRNA (Figs.…”

Section: Galanin Receptor 1 Is Expressed In Glutamatergic Cells In Th...supporting

confidence: 89%

“…In addition to NA, LC neurons co-express several neuropeptides, particularly galanin which is found in eighty percent of those neurons in the rat [15]. This co-existence and the presence of galanin receptors in regions such as the prefrontal cortex and hippocampus [16,17] strongly implicate this neuropeptide in the noradrenergic modulation of cognitive control processes.…”

Section: Introductionmentioning

confidence: 97%

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Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Messanvi,

Visocky,

Senneca

et al. 2024

Preprint

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While amino acid neurotransmitters are the main chemical messengers in the brain, they are co-expressed with neuropeptides which are increasingly recognized as modulators of cognitive pathways. For example, the neuropeptide galanin has been implicated in a wide range of pathological conditions in which frontal and temporal structures are compromised. In a recent study in rats, we discovered that direct pharmacological stimulation of galanin receptor type 1 (GalR1) in the ventral prefrontal cortex (vPFC) and ventral hippocampus (vHC) led to opposing effects on attention and impulse control behavior. In the present study, we investigate how subtypes of neurons expressing GalR1 in these two areas differentially contribute to these behaviors. We first establish that GalR1 is predominantly expressed in glutamatergic neurons in both the vPFC and HC. We develop a novel viral approach to gain genetic access to GalR1-expressing neurons and demonstrate that optogenetic excitation of GalR1 expressing neurons in the vPFC, but not vHC, selectively disrupts attention in a complex behavioral task. Finally, using fiber photometry, we measure the bulk calcium dynamics in GalR1-expressing neurons during the same task to demonstrate opposing activity in vPFC and vHC. These results are consistent with our previous work demonstrating differential behavioral effects induced by GalR1 activating in vPFC and vHC. These results indicate the distinct neuromodulatory and behavioral contributions of galanin mediated by subclasses of neurons in the hippocampal and prefrontal circuitry.

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Section: Galanin Receptor 1 Is Expressed In Glutamatergic Cells In Th...supporting

confidence: 89%

Section: Introductionmentioning

confidence: 97%

Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Messanvi,

Visocky,

Senneca

et al. 2024

Preprint

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“…; Barreda‐Gómez et al . ) during which the endogenous LPA ligand could be degraded. Further studies would be necessary to address this possibility.…”

Section: Discussionmentioning

confidence: 99%

Anatomical location of LPA₁ activation and LPA phospholipid precursors in rodent and human brain

Román

Manuel

Chun

et al. 2015

Journal of Neurochemistry

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Lysophosphatidic acid (LPA) is a signaling molecule that binds to six known G protein-coupled receptors (GPCRs): LPA1–LPA6. LPA evokes several responses in the CNS including cortical development and folding, growth of the axonal cone and its retraction process. Those cell processes involve survival, migration, adhesion proliferation, differentiation and myelination. The anatomical localization of LPA1 is incompletely understood, particularly with regard to LPA binding. Therefore, we have used functional [35S]GTPγS autoradiography to verify the anatomical distribution of LPA1 binding sites in adult rodent and human brain. The greatest activity was observed in myelinated areas of the white matter such as corpus callosum, internal capsule and cerebellum. MaLPA1-null mice (a variant of LPA1-null) lack [35S]GTPγS basal binding in white matter areas, where the LPA1 receptor is expressed at high levels, suggesting a relevant role of the activity of this receptor in the most myelinated brain areas. In addition, phospholipid precursors of LPA were localized by MALDI-IMS in both rodent and human brain slices identifying numerous species of phosphatides (PA) and phosphatidylcholines (PC). Both PA and PC species represent potential LPA precursors. The anatomical distribution of these precursors in rodent and human brain may indicate a metabolic relationship between LPA and LPA1 receptors.

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“…In this study, we have analyzed the differences in the [ 35 S]GTPγS binding evoked by some of the most used galanin receptors ligands. For this purpose, an exhaustive anatomical analysis of the activation of galanin GPCRs was performed in rat brain slices, since this is a valid animal model for the study of the activation of galanin receptors by functional autoradiography (Barreda-Gómez et al, 2014). Initially, the stimulation of GalRs evoked by galanin itself was analyzed and, as we previously described, the highest stimulations induced by this neuropeptide were observed in the dorsal part of the anterior olfactory nucleus and in the lateral olfactory tract (Barreda-Gómez et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Neuroanatomical Characterization of the G Protein-Coupled Receptor Activity Evoked by Galanin-Related Ligands

Barreda-Gómez

Manuel

Rodrı́guez-Puertas

2023

Preprint

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Galanin activated Gi/o-proteins in human and rat central nervous systems

Cited by 11 publications

References 53 publications

Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Anatomical location of LPA₁ activation and LPA phospholipid precursors in rodent and human brain

Neuroanatomical Characterization of the G Protein-Coupled Receptor Activity Evoked by Galanin-Related Ligands

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Galanin activated Gi/o-proteins in human and rat central nervous systems

Cited by 11 publications

References 53 publications

Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Galanin receptor 1 expressing neurons in hippocampal-prefrontal circuitry modulate goal directed attention and impulse control

Anatomical location of LPA1 activation and LPA phospholipid precursors in rodent and human brain

Neuroanatomical Characterization of the G Protein-Coupled Receptor Activity Evoked by Galanin-Related Ligands

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Anatomical location of LPA₁ activation and LPA phospholipid precursors in rodent and human brain