Galanin Is a Potent In Vivo Modulator of Mesencephalic Serotonergic Neurotransmission

Kehr, Ján; Yoshitake, Takashi; Wang, Fuhua; Razani, Haleh; Giménez‐Llort, Lydia; Jansson, Anders; Yamaguchi, Masatoshi; Ögren, Sven Ove

doi:10.1016/s0893-133x(02)00309-3

Cited by 85 publications

(69 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed increase in immobility and suppression of climbing are probably not simply related to a decrease in locomotor activity, since i.c.v. galanin only marginally reduces spontaneous locomotor activity in rat (Kehr et al, 2002).…”

Section: Effects Of Fluoxetine and Galanin Receptor Ligands On Depresmentioning

confidence: 85%

“…galanin caused a significant long-lasting reduction of the basal (Kehr et al, 2002) and SSRI-induced (Yoshitake et al, 2003) 5-HT release in the hippocampus, as measured by in vivo microdialysis. Moreover, galanin decreased TPH2 mRNA expression in the DR (Kehr et al, 2002), suggesting that galanin also inhibits 5-HT synthesis. In contrast to a profound inhibition of 5-HT release, i.c.v.…”

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 89%

“…The inhibitory effect of galanin on the DR 5-HT system after exposure to stress probably affects galanin receptor-mediated mechanism, since both the neurochemical and behavioral effects of i.c.v. galanin are blocked by the non-selective galanin antagonist M35 (Kehr et al, 2002;Kuteeva et al, 2007). Also the GalR3 selective antagonists exerted an antidepressant-like effect in several behavioral models relevant for anxiety-and depression-like behavior, including the FST (Swanson et al, 2005;Barr et al, 2006).…”

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 99%

“…However, galanin, as well as galanin-receptor selective ligands may affect depression-like behavior by directly influencing 5-HT neurotransmission. The available data indicate a profound inhibitory effect of galanin on 5-HT transmission under basal conditions, which is probably mediated by the GalR1/GalR3 receptors (Xu et al, 1998a, c;Kehr et al, 2002;Yoshitake et al, 2003;Mazarati et al, 2005;Swanson et al, 2005). The inhibitory effect of galanin on the DR 5-HT system after exposure to stress probably affects galanin receptor-mediated mechanism, since both the neurochemical and behavioral effects of i.c.v.…”

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 99%

“…Electrophysiological, behavioral, and neurochemical studies have shown that galanin exerts modulatory (mainly inhibitory) effects on both the noradrenergic and serotonergic systems (Seutin et al, 1989;Sevcik et al, 1993;Pieribone et al, 1995;Xu et al, 1998c;Razani et al, 2001;Kehr et al, 2002;see Ö gren et al, 2006). Moreover, in vivo galanin can modulate 5-HT 1A preand postsynaptic receptor functions in an antagonistic manner (see Fuxe et al, 1998;Misane et al, 1998; The action of galanin is mediated via three G proteincoupled receptors, GalR1-GalR3 (see Branchek et al, 2000), which are expressed in the LC, DR, and their projection areas (Xu et al, 1998b, c;O'Donnell et al, 1999;Burazin et al, 2000;Larm et al, 2003;Hawes and Picciotto, 2004;Hawes et al, 2005;Swanson et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Differential Role of Galanin Receptors in the Regulation of Depression-Like Behavior and Monoamine/Stress-Related Genes at the Cell Body Level

Kuteeva

Wardi

Lundström

et al. 2008

Neuropsychopharmacol

View full text Add to dashboard Cite

The present study on rat examined the role of galanin receptor subtypes in regulation of depression-like behavior as well as potential molecular mechanisms involved in the locus coeruleus (LC) and dorsal raphe (DR). The effect of intracerebroventricular (i.c.v.) infusion of galanin or galanin receptor GalR1-and GalR2-selective ligands was studied in the forced swim test, followed by quantitative in situ hybridization studies. Naive control, non-treated (swim control), saline-and fluoxetine-treated rats were used as controls in the behavioral and in situ hybridization studies. Subchronic treatment with fluoxetine reduced immobility and climbing time. Intracerebroventricular infusion of galanin, the GalR1 agonist M617 or the GalR2 antagonist M871 increased, while the GalR2(R3) agonist AR-M1896 decreased, immobility time compared to the aCSF-treated animals. Galanin also decreased the time of climbing. Galanin mRNA levels were upregulated by the combination of injection + swim stress in the saline-and the fluoxetine-treated groups in the LC, but not in the DR. Also tyrosine hydroxylase levels in the LC were increased following injection + swim stress in the saline-and fluoxetine-treated rats. Tryptophan hydroxylase 2 and serotonin transporter mRNAs were not significantly affected by any treatment. 5-HT 1A mRNA levels were downregulated following i.c.v. galanin, M617 or AR-M1896 infusion. These results indicate a differential role of galanin receptor subtypes in depression-like behavior in rodents: GalR1 subtype may mediate 'prodepressive' and GalR2 'antidepressant' effects of galanin. Galanin has a role in behavioral adaptation to stressful events involving changes of molecules important for noradrenaline and/or serotonin transmission.

show abstract

Section: Effects Of Fluoxetine and Galanin Receptor Ligands On Depresmentioning

confidence: 85%

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 89%

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 99%

Section: Integrative Mechanism Of Galanininergic Regulation Of Depresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differential Role of Galanin Receptors in the Regulation of Depression-Like Behavior and Monoamine/Stress-Related Genes at the Cell Body Level

Kuteeva

Wardi

Lundström

et al. 2008

Neuropsychopharmacol

View full text Add to dashboard Cite

show abstract

The Noradrenergic System

2019

Chemical Biology of Neurodegeneration

View full text Add to dashboard Cite

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

Yoshitake

Kehr

Todoroki

et al. 2005

Biomedical Chromatography

103

View full text Add to dashboard Cite

The present paper provides an overview on currently developed derivatization chemistries and techniques for determination of monoamine neurotransmitters serotonin (5-HT), norepinephrine (NE) and dopamine (DA) in microdialysis samples by microbore liquid chromatography with fluorescence detection. In mild alkaline conditions, 5-hydroxyindoles and catecholamines react with benzylamine (BA), forming highly fluorescent 2-phenyl-4,5-pyrrolobenzoxazoles and 2-phenyl(4,5-dihydropyrrolo) [2,3-f]benzoxazoles, respectively. However, for derivatization of DA a higher fluorescence intensity was achieved for reaction with 1,2-diphenylethylenediamine (DPE) rather than with BA, therefore for simultaneous determination of 5-HT, NE and DA in brain microdialysates, a two-step derivatization with BA followed by DPE was developed. The detection limits for 5-HT, NE and DA were 0.2, 0.08 and 0.13 fmol, respectively, in an injection volume of 20 microL, which corresponds to concentrations of 30, 12 and 19.5 pm, respectively in standard solution prior to derivatization. The experimental data presented demonstrate the ability of the technique to simultaneously monitor neuronally releasable pools of monoamine neurotransmitters in the rat and mouse brains at basal conditions and following pharmacological treatments or physiological stimuli. These techniques play an important role in drug discovery and clinical investigation of psychiatric and neurological diseases such as depression, schizophrenia and Parkinson's disease.

show abstract

Galanin Is a Potent In Vivo Modulator of Mesencephalic Serotonergic Neurotransmission

Cited by 85 publications

References 59 publications

Differential Role of Galanin Receptors in the Regulation of Depression-Like Behavior and Monoamine/Stress-Related Genes at the Cell Body Level

Differential Role of Galanin Receptors in the Regulation of Depression-Like Behavior and Monoamine/Stress-Related Genes at the Cell Body Level

The Noradrenergic System

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

Contact Info

Product

Resources

About