Galanthamine Pattern in<i>Narcissus confusus</i>Plants

López, S.; Viladomat, Françesc; Codina, Carles

doi:10.1055/s-2003-818013

Cited by 17 publications

(3 citation statements)

References 5 publications

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“…Taking into consideration the dynamic secondary metabolite profile in the plant based on the lifecycle, it is not surprising to determine higher alkaloid quantities in aerial parts of the plant collected in the last term of the flowering season. In furtherance of our results, Narcissus confusus Pugsley collected from Spain has been investigated in terms of galanthamine-type alkaloid content in different developmental stages and leaves were reported as alkaloid-rich part comparing to the bulbs in flowering senescence period [24].…”

Section: Discussionsupporting

confidence: 58%

Simultaneous quantitative analysis of biologically important Amaryllidaceae alkaloids in Narcissus tazetta L. subsp. tazetta by HPLC/PDA

Karakoyun¹,

Unver-Somer²

2019

jrp

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The present study was conducted for the quantitative determination of biologically active Amaryllidaceae alkaloids, galanthamine and lycorine, in the bulbs and aerial parts of a subspecies naturally growing in Turkey namely, Narcissus tazetta L. subsp. tazetta collected from Muğla/Turkey. A reverse phase high performance liquid chromatographic method using an isocratic system consisting of trifluoroacetic acid:water:acetonitrile (0.01:90:10) with a flow rate of 1 mL/min was carried out. Photodiode array detection was employed to determine the UV-visible spectral characteristics of the alkaloids. Determination of acetylcholinesterase and butyrylcholinesterase inhibitory activities of different parts of mentioned subspecies were accomplished using a microplate assay modified from Ellman's method. This is the first report of the galanthamine content of Narcissus tazetta subsp. tazetta of Turkish origin. 0.0051% and 0.0055% galanthamine was detected in the bulbs and aerial parts of the plant, respectively. Also, lycorine was determined as 0.0250% in the bulbs and 0.0672 % in the aerial parts.

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Section: Discussionsupporting

confidence: 58%

Simultaneous quantitative analysis of biologically important Amaryllidaceae alkaloids in Narcissus tazetta L. subsp. tazetta by HPLC/PDA

Karakoyun¹,

Unver-Somer²

2019

jrp

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“…pseudonarcissus ‘Carlton’ and N . confusus where highest amount of AAs occurred in bulbs 47 – 51 . Our QTOF-MS data showed predominantly ortho-para’ ( i .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolome profiling of Narcissus pseudonarcissus ‘King Alfred’ reveal components of Amaryllidaceae alkaloid metabolism

Singh

Desgagné‐Penix

2017

Sci Rep

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Amaryllidaceae alkaloids (AAs) represent a diverse class of plant specialized metabolites and many display potent pharmacological activities. The AA metabolic pathway is poorly understood and resources are minimal. To enable AA pathway elucidation and novel biosynthetic enzymes discovery, we generated comprehensive metabolomic and corresponding transcriptomic datasets from different tissues of Narcissus pseudonarcissus ‘King Alfred’. In this study, we performed untargeted UPLC-QTOF-MS metabolite analysis from different tissues, which generated exhaustive list of compounds, including several AAs, most predominant and diverse in bulbs. RNA sequencing of N. pseudonarcissus ‘King Alfred’ bulbs yielded 195,347 transcripts, after assembly. Top expressed genes belong to process like metabolism, survival, and defense including alkaloid biosynthetic genes. The transcriptome contained complete sequences for all proposed genes encoding AA-biosynthetic enzymes such as tyrosine decarboxylase (TYDC1 and TYDC2), phenylalanine ammonia-lyase (PAL1 and PAL2) and phenolic acids hydroxylases (C4H and C3H) to name a few. Furthermore, transcriptome data were validated using RT-qPCR analysis and expression study in different tissues of N. pseudonarcissus ‘King Alfred’ was performed. Here, we present the first comprehensive metabolome and transcriptome study from N. pseudonarcissus ‘King Alfred’ providing invaluable resources for metabolic engineering and biotechnological applications.

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“…In view of this finding, poppies might be perceived as alternative sources and be introduced into the treatment strategies for impaired cognition, because the percentage content of this alkaloid exceeded 0.77 % of the dried weight. According to Lopez et al [ 26 ], the highest concentration of galanthamine in Narcissus confusus bulbs was calculated to be 2.5 % at most. The ESI-TOF-MS spectrum of galanthamine (Fig.…”

Section: Resultsmentioning

confidence: 99%

Application of hydrostatic CCC–TLC–HPLC–ESI-TOF-MS for the bioguided fractionation of anticholinesterase alkaloids from Argemone mexicana L. roots

Kukuła-Koch

Mroczek

2015

Anal Bioanal Chem

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A rapid hydrostatic counter-current chromatography–thin-layer chromatography–electrospray-ionization time-of-flight mass spectrometry (CCC–TLC–ESI-TOF-MS) technique was established for use in seeking potent anti-Alzheimer’s drugs among the acethylcholinesterase inhibitors in Argemone mexicana L. underground parts, with no need to isolate components in pure form. The dichloromethane extract from the roots of Mexican prickly poppy that was most rich in secondary metabolites was subjected to hydrostatic-CCC-based fractionation in descending mode, using a biphasic system composed of petroleum ether–ethyl acetate–methanol–water at the ratio of 1.5:3:2.1:2 (v/v). The obtained fractions were analyzed in a TLC-based AChE-inhibition “Fast Blue B” test. All active components in the fractions, including berberine, protopine, chelerithrine, sanguinarine, coptisine, palmatine, magnoflorine, and galanthamine, were identified in a direct TLC–HPLC–ESI-TOF-MS assay with high accuracy. This is the first time galanthamine has been reported in the extract of Mexican prickly poppy and the first time it has been identified in any member of the Papaveraceae family, in the significant quantity of 0.77 %.Graphical AbstractScreening platform of anti-AChE alkaloids

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Galanthamine Pattern inNarcissus confususPlants

Cited by 17 publications

References 5 publications

Simultaneous quantitative analysis of biologically important Amaryllidaceae alkaloids in Narcissus tazetta L. subsp. tazetta by HPLC/PDA

Simultaneous quantitative analysis of biologically important Amaryllidaceae alkaloids in Narcissus tazetta L. subsp. tazetta by HPLC/PDA

Transcriptome and metabolome profiling of Narcissus pseudonarcissus ‘King Alfred’ reveal components of Amaryllidaceae alkaloid metabolism

Application of hydrostatic CCC–TLC–HPLC–ESI-TOF-MS for the bioguided fractionation of anticholinesterase alkaloids from Argemone mexicana L. roots

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