Galectin-3-independent Down-regulation of GABABR1 due to Treatment with Korean Herbal Extract HAD-B Reduces Proliferation of Human Colon Cancer Cells

Kim, Kyung‐Hee; Kwon, Yong-Kyun; Cho, Chong-Kwan; Lee, Yeon‐Weol; Lee, SoHyun; Jang, Sang-Geun; Yoo, Byong Chul; Yoo, Hwa-Seong

doi:10.3831/kpi.2012.15.002

Cited by 11 publications

(7 citation statements)

References 30 publications

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“…Several studies supported the anticancer effect of HAD-B in various cancer cells including human NSCLC cells. 15 - 19 HAD-B1, a herbal aqueous extract, composed of 4 critical herbs (Panax Notoginseng Radix, Cordyceps militaris, Panax ginseng CA Mey, and Boswellia carteri Birdw), was developed to focus on lung cancer treatment. This study was conducted to investigate the anticancer effects of the HAD-B1 combined with afatinib on H1975 EGFR-L858R/T790M double mutation lung cancer cells with the biological mechanism and solid tumor growth in nude mice bearing a H1975 human lung cancer xenograft.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of HangAmDan-B1 (HAD-B1) Combined With Afatinib on H1975 Lung Cancer Cell–Bearing Mice

et al. 2019

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Epidermal growth factor receptor mutation-positive non–small cell lung cancer is cared for mainly by target therapeutics in the clinical treatment at present. We investigated the antitumor effect of HangAmDan-B1 (HAD-B1) combined with afatinib on H1975 (L858R/T790M double mutation) lung cancer cells. The combined treatment of HAD-B1 with afatinib inhibited the proliferation of H1975 cells in a dose-dependent manner compared with the treatment of afatinib or HAD-B1 alone. The combined treatment group significantly induced early apoptosis and cell cycle arrest of the cells compared with afatinib- or HAD-B1-treated control group. Profile analysis of cell cycle proteins in H1975 cells treated with the combination of HAD-B1 and afatinib using InnoPharmaScreen antibody microarray showed downregulation of pERK1/2 and upregulation of p16 in the cells. In vivo tumor growth assay in xenograft animal model of human H1975 lung cancer cells revealed that the mean tumor volume in the group treated with the combination of HAD-B1 and afatinib showed a significant reduction compared with the control groups.

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Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of HangAmDan-B1 (HAD-B1) Combined With Afatinib on H1975 Lung Cancer Cell–Bearing Mice

et al. 2019

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“…Pinto et al [28] indicated that there is a complicated cell communication in response to ionizing radiation revealed by primary human macrophage-cancer cell culture. Kim et al [29] reported that IFITM1 expression was positively correlated with galectin-3 via receptor signaling protein activity in human colon cancer cells. The cell junctions might lead to cancer due to the differences in cell junctions for colorectal cancer [30].…”

Section: Discussionmentioning

confidence: 99%

Predicting MicroRNA Target Genes and Identifying Hub Genes in IIA Stage Colon Cancer Patients Using Bioinformatics Analysis

Dong

Lin

Kujawa

et al. 2019

BioMed Research International

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Background. Colon cancer is a heterogeneous disease, differing in clinical symptoms, epigenetics, and prognosis for each individual patient. Identifying the core genes is important for early diagnoses and it provides a more precise method for treating colon cancer. Materials and Methods. In this study, we wanted to pinpoint these core genes so we obtained GSE101502 microRNA profiles from the GEO database, which resulted in 17 differential expressed microRNAs that were identified by GEO2R analysis. Then, 875 upregulated and 2920 downregulated target genes were predicted by FunRich. GO and KEGG pathway were used to do enrich analysis. Results. GO analysis indicated that upregulated genes were significantly enriched in the regulation of cell communication and signaling and in nervous system development, while the downregulated genes were significantly enriched in nervous system development and regulation of transcription from the RNA polymerase II promoter. KEGG pathway analysis suggested that the upregulated genes were enriched in axon guidance, MAPK signaling pathway, and endocytosis, while the downregulated genes existed in pathways in cancer, focal adhesion, and PI3K-Akt signaling pathway. The top four molecules including 82 hub genes were identified from the PPI network and involved in endocytosis, spliceosome, TGF-beta signaling pathway, and lysosome. Finally, NUDT21, GNB1, CLINT1, and COL1A2 core gene were selected due to their correlation with the prognosis of IIA stage colon cancer. Conclusion. this study suggested that NUDT21, GNB1, CLINT1, and COL1A2 might be the core genes for colon cancer that play an important role in the development and prognosis of IIA stage colon cancer.

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“…Previously, antitumor effects of HAD-B were investigated in several in vitro models ( 7 – 10 ). However, to the best of our knowledge, there is no preceding report on the in vivo antitumor effect of HAD-B.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported the anti-angiogenic and anti-metastatic effects of HAD-B ( 7 , 8 ). In addition, the suppression of γ-aminobutyric acid B receptor 1 (GABABR1) and heat shock protein 27 (HSP27) expression was demonstrated as a molecular mechanism underlying the anti-cancer effect of HAD-B ( 9 , 10 ). However, previous studies have not provided evidence on the immunomodulatory action of HAD-B.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of lung cancer growth by HangAmDan-B is mediated by macrophage activation to M1 subtype

et al. 2017

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Re-education of tumor-associated macrophages (TAMs) toward antitumor effectors may be a promising therapeutic strategy for the successful treatment of cancer. HangAmDan-B (HAD-B), a herbal formula, has been used for stimulating immune function and activation of vital energy to cancer patients in traditional Korean Medicine. Previous studies have reported the anti-angiogenic and anti-metastatic effects of HAD-B; however, evidence on the immunomodulatory action of HAD-B was not demonstrated. In the present study, immunocompetent mice were used to demonstrate the suppression of the in vivo growth of allograft Lewis lung carcinoma (LLC) cells, by HAD-B. In addition, HAD-B inhibited the in vitro growth of LLC cells by driving macrophages toward M1 polarization, but not through direct inhibition of tumor cell growth. Furthermore, culture media transfer of HAD-B-treated macrophages induced apoptosis of LLC cells. Results of the present study suggest that the antitumor effect of HAD-B may be explained by stimulating the antitumor function of macrophages. Considering the importance of re-educating TAMs in the regulation of the tumor microenvironment, the present study may confer another option for anti-cancer therapeutic strategy, using herbal medicines such as HAD-B.

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Galectin-3-independent Down-regulation of GABABR1 due to Treatment with Korean Herbal Extract HAD-B Reduces Proliferation of Human Colon Cancer Cells

Cited by 11 publications

References 30 publications

Inhibitory Effects of HangAmDan-B1 (HAD-B1) Combined With Afatinib on H1975 Lung Cancer Cell–Bearing Mice

Inhibitory Effects of HangAmDan-B1 (HAD-B1) Combined With Afatinib on H1975 Lung Cancer Cell–Bearing Mice

Predicting MicroRNA Target Genes and Identifying Hub Genes in IIA Stage Colon Cancer Patients Using Bioinformatics Analysis

Inhibition of lung cancer growth by HangAmDan-B is mediated by macrophage activation to M1 subtype

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