Galectin-8 is expressed by villous and extravillous trophoblast of the human placenta

Kolundžić, Nikola; Bojić‐Trbojević, Žanka; Radojčić, Lj.; Petronijević, Miloš; Vićovac, Ljiljana

doi:10.1016/j.placenta.2011.07.087

Cited by 30 publications

(31 citation statements)

References 19 publications

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“…A long standing interest of this group has been the relevance of galectins for the invasive properties of normal and transformed trophoblast. Gal-1, gal-3, and gal-8 are expressed by the invasive extravillous trophoblast of the cell column [18], [19], [23] and the immortalized invasive trophoblast cell line HTR-8/SVneo [36]. Therefore, in the present investigation the issue of potential participation of gal-1 in human trophoblast cell invasion in vitro using cell models was examined.…”

Section: Discussionmentioning

confidence: 97%

“…Gal-1 is also abundantly expressed in endometrium, as well as in the decidua of early gestation [19], [20]. Other members of the galectin family, gal-3, gal-8, and gal-13, were also reported in human placenta [18], [21]–[23]. In mice [24], [25] gal-1 is synthesized prior to implantation in the trophectoderm of the expanded blastocysts, suggesting a role in the attachment of the embryo to the uterine epithelium [24].…”

Section: Introductionmentioning

confidence: 96%

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Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

et al. 2011

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BackgroundInteractions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it.Methods and FindingsFunction blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM±6.3, P<0.001) and to 66% of control (SEM±1.7, P<0.001) in HTR-8/SVneo cell line. Increased availability of gal-1, as two molecular forms of recombinant human gal-1 (CS-gal-1 and Ox-gal-1), resulted in increased cell invasion by cytotrophoblast to 151% (SEM±16, P<0.01) with 1 ng/ml of CS-gal-1, and to 192% (SEM±51, P<0.05) with 1 µg/ml of Ox-gal-1. Stimulation was also observed in HTR-8/SVneo cells, to 317% (SEM±58, P<0.001) by CS-gal-1, and to 200% (SEM±24, P<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests.Conclusion and SignificanceThese findings qualify gal-1 as a member of human trophoblast cell invasion machinery.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

et al. 2011

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“…The negative correlation between expression and trophoblast invasiveness is in accordance with our finding of decreased galectin 3 expression over gestation. Galectin 8 is expressed by decidual cells, villous and extravillous trophoblasts [32], but its role is less clearly understood.…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression at the Maternal-Fetal Interface in Preeclampsia Is Influenced by Gestational Age

Lian

Langaas²,

Moses

et al. 2013

PLoS ONE

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Genome-wide transcription data of utero-placental tissue has been used to identify altered gene expression associated with preeclampsia (PE). As many women with PE deliver preterm, there is often a difference in gestational age between PE women and healthy pregnant controls. This may pose a potential bias since gestational age has been shown to dramatically influence gene expression in utero-placental tissue. By pooling data from three genome-wide transcription studies of the maternal-fetal interface, we have evaluated the relative effect of gestational age and PE on gene expression. A total of 18,180 transcripts were evaluated in 49 PE cases and 105 controls, with gestational age ranging from week 14 to 42. A total of 22 transcripts were associated with PE, whereas 92 transcripts with gestational age (nominal P value <1.51*10−6, Bonferroni adjusted P value <0.05). Our results indicate that gestational age has a great influence on gene expression both in normal and PE-complicated pregnancies. This effect might introduce serious bias in data analyses and needs to be carefully assessed in future genome-wide transcription studies.

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“…38 Galectin-8 is expressed in villous and extravillous trophoblasts. 39 Together, these data suggest that galectin-8 most likely contributes to cellular proliferation, adhesion, invasion, and protection from microbial infection, all controlled by the maternal LGALS8 gene.…”

Section: Discussionmentioning

confidence: 86%

Using RNA sequencing for identifying gene imprinting and random monoallelic expression in human placenta

et al. 2014

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Given the possible critical importance of placental gene imprinting and random monoallelic expression on fetal and infant health, most of those genes must be identified, in order to understand the risks that the baby might meet during pregnancy and after birth. Therefore, the aim of the current study was to introduce a workflow and tools for analyzing imprinted and random monoallelic gene expression in human placenta, by applying whole-transcriptome (WT) RNA sequencing of placental tissue and genotyping of coding DNA variants in family trios. Ten family trios, each with a healthy spontaneous single-term pregnancy, were recruited. Total RNA was extracted for WT analysis, providing the full sequence information for the placental transcriptome. Parental and child blood DNA genotypes were analyzed by exome SNP genotyping microarrays, mapping the inheritance and estimating the abundance of parental expressed alleles. Imprinted genes showed consistent expression from either parental allele, as demonstrated by the SNP content of sequenced transcripts, while monoallelically expressed genes had random activity of parental alleles. We revealed 4 novel possible imprinted genes (LGALS8, LGALS14, PAPPA2 and SPTLC3) and confirmed the imprinting of 4 genes (AIM1, PEG10, RHOBTB3 and ZFAT-AS1) in human placenta. The major finding was the identification of 4 genes (ABP1, BCLAF1, IFI30 and ZFAT) with random allelic bias, expressing one of the parental alleles preferentially. The main functions of the imprinted and monoallelically expressed genes included: i) mediating cellular apoptosis and tissue development; ii) regulating inflammation and immune system; iii) facilitating metabolic processes; and iv) regulating cell cycle.

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Galectin-8 is expressed by villous and extravillous trophoblast of the human placenta

Cited by 30 publications

References 19 publications

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

Differential Gene Expression at the Maternal-Fetal Interface in Preeclampsia Is Influenced by Gestational Age

Using RNA sequencing for identifying gene imprinting and random monoallelic expression in human placenta

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