Galectina-3 em tumores de próstata: imuno-histoquímica e análise digital de imagens

Araújo-Filho, Jorge Luiz Silva; Melo-Júnior, Mário Ribeiro de; Lins, Consuelo Antunes Barreto; Lins, Romero Antunes Barreto; Machado, Marcos Cezar Feitosa de Paula; Carvalho-jr, Luiz Bezerra de; Filho, Nicodemos Teles de Pontes

doi:10.1590/s1676-24442006000600011

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Digital Pathology systems have been used in Latin America for morphometric analysis (Sanz Pupo et al 2006), histology (Araújo et al 2009), histochemistry (Melo-Júnior et al 2006, immunohistochemistry (Araújo-Filho et al 2006;Silva Júnior et al 2009), neuropathology (Peixoto-Santos and Blumcke 2021), medical education (Monteiro et al 2015;Pérez et al 2016), and reproducibility research (Schettini et al 2011). Besides those reports, the experts in the panel also report utilizing DP systems for measurements of cancer invasive borders, static cytometry, intra-operatory biopsy analyses, creation of digital archives, telepathology, research, publications, and videoconferencing.…”

Section: Current Dp Uses In Latin Americamentioning

confidence: 99%

Digital pathology in Latin America

et al. 2023

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Digital pathology (DP) adoption in Latin America has expanded slower than in developed regions, probably due to many barriers not seen in the latter areas. This article aims to present the current scenario in the region, highlighting barriers and possible solutions to encourage its adoption in Latin American countries.Methods An expert panel of 9 Latin American medical pathologists and 1 information technology specialist participated in an online modified Delphi panel, utilizing a third-party platform (iAdvise, Within3, USA). Thirteen pre-prepared questions were answered interactively.Results Experts' observations confirm the paucity of labs in the region that utilize digital pathology technology. The panel ranked obtaining second opinions and presenting images remotely as the main benefit of a digital pathology system, although many others were cited as well. Cost of implantation was the main barrier mentioned by the experts. Payers' and decision makers' lack of awareness of benefits ranked second as a barrier to DP implementation. Internet infrastructure was also mentioned as a concerning issue in the region. Besides diagnostic pathology services, proposed revenue incomes included commercialization of digital services to other institutions, loan agreements of equipment and software, and organizing courses for pathologists or residents. The need for alternative reimbursement methods for diagnostic services was also mentioned. A regional network of collaborating institutions was also suggested as a viable solution to reach distant areas and laboratories lacking the technology.Conclusions The benefits of DP are clear to the expert panel, but cost and lack of awareness of its benefit may be hampering its widespread adoption in Latin America.

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Section: Current Dp Uses In Latin Americamentioning

confidence: 99%

Digital pathology in Latin America

et al. 2023

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“…Neste presente trabalho, a expressão das proteínas Ki-67 e p-53 foi observada no núcleo e/ou citoplasma nas células de todos os tipos de lesão examinados, este padrão de marcação encontrado neste estudo assemelha-se a outros trabalhos realizados em nossos laboratórios que avaliaram a expressão tecidual de outras proteínas, como a galectina 3 em câncer de próstata 43 , e mais recentemente, em pacientes portadores de RCUI 44 . Com o intuito de descrever parâmetros qualitativos e quantitativos mais confiáveis sobre alterações histopatológicas nosso grupo de pesquisas tem desenvolvido metodologias morfométricas diversas [45][46][47] .…”

Section: Proteínaunclassified

Perfil imunoistoquímico das proteínas p53 e Ki67 em pacientes com retocolite ulcerativa inespecífica

Machado

Melo-Júnior

Pontes-Filho

et al. 2010

Rev bras. colo-proctol.

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Comparative Analysis of Extracellular Matrix and Cellular Carbohydrate Expression in the Sporotrichosis and Chromoblastomycosis

et al. 2011

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This work was based on the analysis of digital images of histochemical profile from subcutaneous lesions in sporotrichosis (ST) and chromoblastomycosis (CM) patients. An additional aim was the detection of carbohydrate expression using lectin histochemical analysis of the different carbohydrates in the fungal cell wall from four different species (Sporothrix schenckii, Fonsecaea pedrosoi, Phialophora verrucosa, and Cladophialophora carrionii) associated with diseases mentioned earlier. Slides from tissue biopsies from ST and CM positive patients (n=10, each) were stained according to routine techniques. Slides were incubated with 25 μg/ml of Con A lectins and WGA conjugated to peroxidase. Digital image analysis was carried out in a workstation using OPTIMAS™ software system. Routine histochemistry results indicated that there is significantly higher collagen deposition and elastic fibers in ST characteristic lesions compared with that found in CM cases. The ST interstitial fibrosis area was larger than in CM lesions. Comparative lectin binding showed a positive and intense lectin staining pattern in the cell wall of S. schenckii, suggesting a higher expression of glucose/mannose and N-acetyl glucosamine in their cell surface as evidenced by Con A and WGA, respectively. However, these lectins were not effective to recognize some carbohydrates moieties in the F. pedrosoi, P. verrucosa, and C. carrionii. Such findings contribute to additional information about specific recognition processes between fungal parasites and their host cell targets may be mediated by the interaction of carbohydrate-binding proteins, such as lectins, on the surface of one type of cell that combine with complementary sugars on the surface of another cells into fibro-connective tissues associated with lesions.

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Galectina-3 em tumores de próstata: imuno-histoquímica e análise digital de imagens

Abstract: Primeira submissão em 29/06/06 Última submissão em 15/09/06 Aceito para publicação em 24/10/06 Publicado em 20/12/06

Cited by 4 publications

References 25 publications

Digital pathology in Latin America

Digital pathology in Latin America

Perfil imunoistoquímico das proteínas p53 e Ki67 em pacientes com retocolite ulcerativa inespecífica

Comparative Analysis of Extracellular Matrix and Cellular Carbohydrate Expression in the Sporotrichosis and Chromoblastomycosis

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