Galega officinalis extract regulate the diabetes mellitus related violations of proliferation, functions and apoptosis of leukocytes

Abstract

BackgroundAn impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections. The outmost importance for the understanding of the immunological processes involved in diabetes pathogenesis is to give the characteritics of the immunological profile and changes therein, during the course of desease. Long-used in folk medicine to treat diabetes Galega officinalis L. has been chosen for the correction of the immune system dysfunction.MethodsThe experiments were conducted on male Wistar rats. Fractionation of bone marrow cells suspension was performed in a three-layer ficoll–sodium amidotrizoate density gradient. The lymphocytic-granulocytic cells proliferative activity was studied using enzyme immunoassay with 5-bromo-2′-deoxyuridine (BrdU). For staining of bone marrow preparations May-Gruenwald-Romanowsky-Giemsa (Pappenheim) method was used. To evaluate the content of cationic proteins and myeloperoxidase in neutrophilic leukocytes cytochemical studies were performed. Content of tumor necrosis factor alpha was carried out by immuno-enzymatic analysis. Lymphocytes apoptosis was examined by fluorescent analysis using annexin V.ResultsDiabetes mellitus development was accompanied with violation of neutrophils and lymphocytes proliferation, increased activity of myeloperoxidase and enhanced apoptosis process. Administration of Galega officinalis extract under the condition of diabetes promotes the restoration of neutrophils bone marrow pool and the reduction of lymphoblasts number and causes inhibition of the lymphocytes apoptosis process.ConclusionsInvestigated medicine has a pronounced immunocorrective effect under the conditions of diabetes mellitus and can become the basis for creating a new generation of antidiabetic drugs.

A Novel Method for Extraction of Galegine by Molecularly Imprinted Polymer (MIP) Technique Reinforced with Graphene Oxide and Its Evaluation Using Polarography

“…Other compounds derived from Galega include flavonoids, glycosides, alkaloids, 4-hydroxygalgens, tannins, saponins, sucrose, and fatty oils [7]. Galegine reduces blood sugar [8] and has an anticoagulant effect [9], decreases blood pressure [10], and increases the production of milk in sheep [11]. Due to the importance of galegine and its small amount in the plant, a method of dissecting should be used in order to extract with the high purity that its sensitivity, repeatability, and the measurement accuracy should be high.…”

Functional activities of neutrophils in diabetic rats are changed by yacon extracts

“…To test the phagocytic activity, rats' neutrophils were allowed to phagocytize unopsonized yeast cells. Changes in phagocytosis activity indexes ( Table 1, Figure 1) indicate that neutrophils require more time for an adequate response to foreign cells and particles [5].…”

“…Preparation of water extracts of yacon was performed according to Nagalievska et al [5]. For the research water solutions of evaporated extracts were used.…”

The Protective Effect of Germanium Citrate on Functional State of Immune Cells and Neutrophil Activity Under the Condition of Lipolysaccharide Induced Inflammation

“…Оцінювали 100 нейтрофілів у препаратах крові мишей із розрахунком відсотка формазанпозитивних клітин та цитохімічного показника (ЦХП) за формулою: ЦХП = (А·0 + В·1 + С·2 + Д·3)/100, де А, В, С і Д -кількість нейтрофілів: А -без формазану або його дуже мало; В -площа відкладень формазану не перевищує 1/3 від такої ядра клітини; С -відкладення займають від 1/3 до усієї площі ядра клітини; Д -площа включень формазану більша за площу ядра [14]. Функціональний стан нейтрофілів оцінювали також за результатом напівкількісного лізосомально-катіонного тесту (ЛКТ) із розрахунком середнього цитохімічного коефіцієнту (СЦК) [15].…”