Gallic acid ameliorates atherosclerosis and vascular senescence and remodels the microbiome in a sex-dependent manner in ApoE−/− mice

Clark, McKenzie; Centner, Ann; Ukhanov, Vladimir; Nagpal, Ravinder; Salazar, Gloria

doi:10.1016/j.jnutbio.2022.109132

Cited by 29 publications

(28 citation statements)

References 74 publications

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“…The gut microbiome will be measured as per our previously described methods (4,12,(23)(24)(25)(26)(27). Briefly, genomic DNA from 200 mg of the fecal specimen was extracted using the QIAmp PowerFecal Pro DNA Kit (Qiagen) according to the manufacturer's instructions.…”

Section: Gut Microbiome Analysismentioning

confidence: 99%

Prebiotic mechanisms of resistant starches from dietary beans and pulses on gut microbiome and metabolic health in a humanized murine model of aging

et al. 2023

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Dietary pulses, being a rich source of fiber and proteins, offer an ideal and inexpensive food choice for older adults to promote gut and metabolic health. However, the prebiotic effects of dietary pulses-derived resistant starches (RS), compared to RS from cereals and tubers, remain relatively underexplored, particularly in context to their gut modulatory potential in old age. We herein investigate the prebiotic effects of pulses-derived RS on the gut microbiome and intestinal health in aged (60-week old) mice colonized with human microbiota. C57B6/J mice were fed for 20 weeks with either a western-style high-fat diet (control; CTL) or CTL diet supplemented (5% w/w) with RS from pinto beans (PTB), black-eyed-peas (BEP), lentils (LEN), chickpeas (CKP), or inulin (INU; reference control). We find that the RS supplementation modulates gut microbiome in a sex-dependent manner. For instance, CKP enriched α-diversity only in females, while β-diversity deviated for both sexes. Further, different RS groups exhibited distinct microbiome differences at bacterial phyla and genera levels. Notably, LEN fostered Firmicutes and depleted Proteobacteria abundance, whereas Bacteroidota was promoted by CKP and INU. Genus Dubosiella increased dominantly in males for all groups except PTB, whilst Faecalibaculum decreased in females by CKP and INU groups. Linear discriminant analysis effect size (LEfSe) and correlational analyzes reveal RS-mediated upregulation of key bacterial genera associated with short-chain fatty acids (butyrate) production and suppression of specific pathobionts. Subsequent machine-learning analysis validate decreased abundance of notorious genera, namely, Enterococcus, Odoribacter, Desulfovibrio, Alistipes and Erysipelatoclostridium among RS groups. CKP and LEN groups partly protected males against post-prandial glycemia. Importantly, RS ameliorated high-fat diet-induced gut hyperpermeability and enhanced expression of tight-junction proteins (claudin-1 and claudin-4), which were more pronounced for LEN. In addition, IL10 upregulation was more prominent for LEN, while TNF-α was downregulated by LEN, CKP, and INU. Together, these findings demonstrate that RS supplementation beneficially modulates the gut microbiome with a reduction in gut leakiness and inflammation, indicating their prebiotic potential for functional food and nutritional applications.

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Section: Gut Microbiome Analysismentioning

confidence: 99%

Prebiotic mechanisms of resistant starches from dietary beans and pulses on gut microbiome and metabolic health in a humanized murine model of aging

et al. 2023

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“…HFHCD is associated with hypercholesterolemia characterized by high LDL-C, low HDL-C, and high TG . On this basis, we measured their serum lipid levels in mice.…”

Section: Resultsmentioning

confidence: 99%

“…HFHCD is associated with hypercholester- olemia characterized by high LDL-C, low HDL-C, and high TG. 28 On this basis, we measured their serum lipid levels in mice. As shown in Figure 2A,B, compared with the Mod group, the serum TC and TG levels in the Con group were significantly decreased (p < 0.01), demonstrating that the HFHCD could induce both hyperlipidemia and hypercholesterolemia in mice.…”

Section: Effects Of Torularhodin On Body Weight Foodmentioning

confidence: 99%

Torularhodin-Loaded Bilosomes Ameliorate Lipid Accumulation and Amino Acid Metabolism in Hypercholesterolemic Mice

Liu

Guo

Cheng

et al. 2023

J. Agric. Food Chem.

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Scope: Hypercholesterolemia is a cause of cardiovascular disease. Torularhodin is a carotenoid, and its entrapment in bilosomes helps to improve its bioavailability. Methods and results: The effects of torularhodin-loaded bilosomes on lipid accumulation, inflammatory response, and serum metabolic profiles in hypercholesterolemic ApoE−/− C57BL/6J mice were investigated by feeding a high-fat, high-cholesterol diet (HFHCD) for 20 weeks. At the same time, mice were gavaged with torularhodin-loaded bilosomes for 10 weeks. The results showed that torularhodin successfully alleviated weight gain and insulin resistance in mice and could also lower blood lipids. Meanwhile, torularhodin improved liver lipid accumulation in mice and modulated inflammatory factors in the "blood−liver−ileum." Nontargeted metabolomics revealed that torularhodin significantly increased the concentrations of L-tryptophan, glyceraldehyde, hypotaurine, pyrophosphate, and niacinamide in serum (p < 0.01). In addition, targeted amino acid metabolomics verification found that torularhodin promoted the metabolism of serum amino acids in mice, particularly for branched-chain amino acids, thereby helping to improve hypercholesterolemia in mice. Finally, interaction network bioinformatics was used to demonstrate that amino acid metabolism represented an important mechanism by which torularhodin improves lipid accumulation and inflammatory response in mice. Conclusions: Torularhodin can improve hypercholesterolemia in HFHCD-fed mice, thereby supporting the feasibility of its usage in food applications for cardiovascular disease prevention.

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“…16S MicroBiome Profiling of the 16S rRNA V3-V4 hypervariable region of cecal samples was performed using Illumina MiSeq platform to compare the microbiota diversity and composition across the cecal DNA samples of six groups (control, Stress-control, Beta-glucan, EPS25, EPS50, D-galactose). The obtained sequences (.fastq files) were processed with the QIIME2 (version 2.2021.2; (accessed on 12 October 2021)) bioinformatics pipeline [ 27 ], in a miniconda environment as per our previously described methods [ 28 , 29 , 30 , 31 ], followed by quality-filtering, adapter-trimming, denoising, and removal of non-chimeric amplicons by using the dada2 pipeline [ 32 , 33 ] using default parameters, as per our previous reports [ 29 , 30 ]. To avoid the bias if any of the sequencing depth, alpha-rarefaction was executed at the lowest sequencing depth.…”

Section: Methodsmentioning

confidence: 99%

“…To avoid the bias if any of the sequencing depth, alpha-rarefaction was executed at the lowest sequencing depth. Amplicon sequence variants (ASVs) were assigned bacterial taxonomic nomenclature by using the Naive Bayes classifier natively installed in dada2 workflow and pre-trained with SILVA reference database (version 138.1; (accessed on 12 October 2021)) with 99% sequence identity threshold [ 33 ], as described earlier [ 29 , 30 ]. The obtained sequence reads were filtered to exclude features annotated as ‘mitochondria’ and ‘chloroplast’.…”

Section: Methodsmentioning

confidence: 99%

Lacticaseibacillus rhamnosus-Derived Exopolysaccharide Attenuates D-Galactose-Induced Oxidative Stress and Inflammatory Brain Injury and Modulates Gut Microbiota in a Mouse Model

et al. 2022

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This study aimed to investigate the protective effect of a novel exopolysaccharide EPSRam12, produced by Lacticaseibacillus rhamnosus Ram12, against D-galactose-induced brain injury and gut microbiota dysbiosis in mice. The findings demonstrate that EPSRam12 increases the level of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase, total antioxidant capacity, and the level of anti-inflammatory cytokine IL-10, while decreasing malonaldehyde, nitric oxide, pro-inflammatory cytokines including TNF-α, IL-1β, IL-6, MCP-1, and the mRNA expression of cyclooxygenase-2, inducible nitric oxide synthase, and the activation of nuclear factor-kappa-B in the brain tissues of D-galactose-treated mice. Further analyses reveal that EPSRam12 improves gut mucosal barrier function and increases the levels of short-chain fatty acids (SCFAs) in the intestine while restoring gut microbial diversity by enriching the abundance of SCFA-producing microbial genera Prevotella, Clostridium, Intestinimonas, and Acetatifactor while decreasing potential pathobionts including Helicobacter. These findings of antioxidative and anti-inflammatory effects in the brain and ameliorative effects on epithelial integrity, SCFAs and microbiota in the gut, provide novel insights into the effect of EPSRam12 intervention on the gut–microbiome–brain axis and should facilitate prospective understanding of microbial exopolysaccharide for improved host health.

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Gallic acid ameliorates atherosclerosis and vascular senescence and remodels the microbiome in a sex-dependent manner in ApoE−/− mice

Cited by 29 publications

References 74 publications

Prebiotic mechanisms of resistant starches from dietary beans and pulses on gut microbiome and metabolic health in a humanized murine model of aging

Prebiotic mechanisms of resistant starches from dietary beans and pulses on gut microbiome and metabolic health in a humanized murine model of aging

Torularhodin-Loaded Bilosomes Ameliorate Lipid Accumulation and Amino Acid Metabolism in Hypercholesterolemic Mice

Lacticaseibacillus rhamnosus-Derived Exopolysaccharide Attenuates D-Galactose-Induced Oxidative Stress and Inflammatory Brain Injury and Modulates Gut Microbiota in a Mouse Model

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