Gallic acid and Catechin induce morphological alterations on the zoonotic parasite Hymenolepis diminuta

Mondal, Chandrani; Mandal, Sudeshna; Saha, Samiparna; Ray, Mrinal Kanti; Lyndem, Larisha M.

doi:10.1007/s00436-023-07929-w

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…The The scolex of cestodes is an important attachment organ to the host and the present study have demonstrated irreversible devastating effects on the structure suggesting the inability of the treated parasites to remain attached within the host body leading to their expulsion. Similar observation was also reported from our laboratory on H. diminuta treated with gallic acid and catechin (Mondal et al 2023). In the current study, loss of tegumental architecture with infoldings and cracks were seen in both FA and SA treated worms which was also reported by Nandi et al (2017) in the tegument of R. tetragona when treated with crude leaf extract of Clerodendrum viscosum.…”

Section: Discussionsupporting

confidence: 89%

In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

Saha,

Mondal,

Mandal

et al. 2024

J Parasit Dis

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The present study is aimed to investigate potential in vitro anthelmintic e cacy of two phenolic compounds Ferulic acid and Sinapic acid against the parasite Hymenolepis diminuta. Adult parasites collected from infected rat's intestine (maintained in our laboratory) were treated with 1, 2.5, 5, 10 and 20 mg/mL concentrations of both the compounds in RPMI-1640 media containing 1% Tween 20. Further, one group was treated in Praziquantel as a reference drug and another group of parasites were kept as control. The e cacy was evaluated on the basis of motility and mortality of the parasites. The paralyzed worms were further processed for the morphological and ultrastructural studies and observed through light and scanning electron microscopy. A signi cant dose-dependent e cacy was found in all treatment and decrease in relative movability value was also recorded in all the concentrations of two compounds treated parasites. The time taken for paralysis in 5 mg/mL of Ferulic acid and 10 mg/mL of Sinapic acid were 1.47 ± 0.04 hours and 0.88 ± 0.03 hours respectively which is accorded with the standard concentration of Praziquantel. Morphological micrographs revealed pronounced distortion and altered topography of scolex and tegument while histological study showed loss of uniform tegumental integrity with folds and cracks in the treated parasites. Further, extensive alteration in the scolex and irrevocable disruption all over the body surface with loss of trapezoid shape, shrinkage of tegument and sloughing off microtriches were observed in electron microscopic study. The study indicated that both the compounds possess strong activity against H. diminuta and further studies are required to understand their detailed mode of action to exploit them as potential alternative candidates for curing helminthiases.

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Section: Discussionsupporting

confidence: 89%

In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

Saha,

Mondal,

Mandal

et al. 2024

J Parasit Dis

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Secondary compounds from Cyrtocarpa procera bark inhibits the biological cycle of Haemonchus contortus: in vitro ovicidal and larvicidal studies

De Jesús-Martínez,

Olmedo-Juárez,

Olivares-Pérez

et al. 2024

Industrial Crops and Products

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In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

Saha,

Mondal,

Mandal

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

The present study is aimed to investigate potential in vitro anthelmintic efficacy of two phenolic compounds Ferulic acid and Sinapic acid against the parasite Hymenolepis diminuta. Adult parasites collected from infected rat’s intestine (maintained in our laboratory) were treated with 1, 2.5, 5, 10 and 20 mg/mL concentrations of both the compounds in RPMI-1640 media containing 1% Tween 20. Further, one group was treated in Praziquantel as a reference drug and another group of parasites were kept as control. The efficacy was evaluated on the basis of motility and mortality of the parasites. The paralyzed worms were further processed for the morphological and ultrastructural studies and observed through light and scanning electron microscopy. A significant dose-dependent efficacy was found in all treatment and decrease in relative movability value was also recorded in all the concentrations of two compounds treated parasites. The time taken for paralysis in 5 mg/mL of Ferulic acid and 10 mg/mL of Sinapic acid were 1.47 ± 0.04 hours and 0.88 ± 0.03 hours respectively which is accorded with the standard concentration of Praziquantel. Morphological micrographs revealed pronounced distortion and altered topography of scolex and tegument while histological study showed loss of uniform tegumental integrity with folds and cracks in the treated parasites. Further, extensive alteration in the scolex and irrevocable disruption all over the body surface with loss of trapezoid shape, shrinkage of tegument and sloughing off microtriches were observed in electron microscopic study. The study indicated that both the compounds possess strong activity against H. diminuta and further studies are required to understand their detailed mode of action to exploit them as potential alternative candidates for curing helminthiases.

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Gallic acid and Catechin induce morphological alterations on the zoonotic parasite Hymenolepis diminuta

Cited by 3 publications

References 46 publications

In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

Secondary compounds from Cyrtocarpa procera bark inhibits the biological cycle of Haemonchus contortus: in vitro ovicidal and larvicidal studies

In vitro anthelmintic efficacy of Ferulic and Sinapic acid against zoonotic cestode Hymenolepis diminuta (Rudolphi, 1819)

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