Gallic acid and omega‐3 fatty acids mitigate epididymal and testicular toxicity in manganese‐treated rats

Owumi, Solomon E.; Danso, Olabisi F.; Nwozo, Sarah Onyenibe

doi:10.1111/and.13630

Cited by 18 publications

(9 citation statements)

References 44 publications

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“…The total proteins of the liver and kidney tissues were estimated following an established protocol 52 to determine the exact concentration of any protein of interest in the tissue. The markers of oxidative and nitrosative stress in the liver and kidney of rats were determined following well-documented protocols: Superoxide dismutase (SOD) activity 53 , 54 ; catalase (CAT) activity 55 , 56 using H 2 O 2 as a substrate; glutathione S-transferase (GST) activity 57 ; glutathione peroxidase (GPx) activity 58 , 59 ; reduced glutathione (GSH) level 60 , 61 . Also, total sulfhydryl (TSH) level 62 , 63 ; xanthine oxidase (XO) activity 63 , 64 ; myeloperoxidase (MPO) activity 18 , 65 ; nitric oxide (NO) level 48 , 66 were also evaluated accordingly.…”

Section: Methodsmentioning

confidence: 99%

Apigeninidin-rich Sorghum bicolor (L. Moench) extracts suppress A549 cells proliferation and ameliorate toxicity of aflatoxin B1-mediated liver and kidney derangement in rats

Owumi

Kazeem

et al. 2022

Sci Rep

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Sorghum bicolor plant has a high abundance of 3-deoxyanthocyanins, flavonoids and other polyphenol compounds that have been shown to offer numerous health benefits. Epidemiological studies have linked increased intake of S. bicolor to reduced risk of certain cancer types, including lung adenocarcinoma. S. bicolor extracts have shown beneficial effects in managing hepatorenal injuries. This study investigated the cytotoxic potential of three apigeninidin-rich extracts of S. bicolor (SBE-05, SBE-06 and SBE-07) against selected cancer cell lines and their ameliorative effect on aflatoxin B1 (AFB1)-mediated hepatorenal derangements in rats. We observed that, among the three potent extracts, SBE-06 more potently and selectively suppressed the growth of lung adenocarcinoma cell line (A549) (IC50 = 6.5 μg/mL). SBE-06 suppressed the expression of STAT3 but increased the expression of caspase 3. In addition, SBE-05, SBE-06 and SBE-07 inhibited oxidative and nitrosative stress, inflammation, and apoptosis and preserved the histoarchitectural networks of the liver and kidney of rats treated with AFB1. These in vitro and in vivo studies indicate the potential of these cheap and readily accessible extracts for cancer therapy and as chemo-preventive agents in preventing aflatoxin-related health issues.

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Section: Methodsmentioning

confidence: 99%

Apigeninidin-rich Sorghum bicolor (L. Moench) extracts suppress A549 cells proliferation and ameliorate toxicity of aflatoxin B1-mediated liver and kidney derangement in rats

Owumi

Kazeem

et al. 2022

Sci Rep

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“…(2019). Glutathione‐s‐transferase—GST—activity was assessed following the method of Habig (Habig et al., 1974), and glutathione peroxidase GPx activity was also evaluated by the previously reported method of Rotruck et al, (Owumi, Danso, et al., 2020; Rotruck et al., 1973). The activity of xanthine oxidase—XO—was measured by Bergmeyer et al, method reported recently (Buege & Aust, 1978; Owumi et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

Decrease in reproductive dysfunction using aflatoxin B1 exposure: a treatment with 3‐indolepropionic acid in albino Wistar rat

et al. 2021

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We assessed the individual and combined consequence of 3‐indolepropionic acid on aflatoxin B1‐induced reproductive toxicity in rats. The experimental cohorts were dosed for four consecutive weeks with aflatoxin B1 (50 μg/kg), 3‐indolepropionic acid (50 mg/kg), and both (aflatoxin B1: 50 μg/kg + 3‐indolepropionic acid: 25 or 50 mg/kg), and the untreated control. Following sacrifice, biomarkers of testicular, epididymal and hypothalamic oxidative status, lipid peroxidation, reactive oxygen and nitrogen species, nitric oxide levels and myeloperoxidase activity were determined. Besides, tumour necrosis factor‐alpha, Bcl‐2 and Bax proteins were also assessed. Aflatoxin B1‐induced testicular, epididymal and hypothalamic oxidative stress was significantly alleviated with 3‐indolepropionic acid co‐treatment. Also, increases in biomarkers of oxidative stress and reduced levels of antioxidants were abated significantly in rats co‐treated with 3‐indolepropionic acid. Aflatoxin B1‐mediated increase in tumour necrosis factor‐alpha, Bax, nitric oxide and myeloperoxidase activity in the examined organs was decreased significantly in aflatoxin B1 and 3‐indolepropionic acid co‐treated rats. Also, 3‐indolepropionic acid dose dependently reduced Bcl‐2 levels in the treated rats. The degree of aflatoxin B1‐induced histopathological injuries was minimised in rats co‐treated with 3‐indolepropionic acid. Our results demonstrated that 3‐indolepropionic acid protected experimental rats from aflatoxin B1‐induced oxido‐inflammatory stress and apoptotic response in the examined organs.

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“…The activities of acid and alkaline phosphatase (ACP and ALP), glucose 6‐phosphate dehydrogenase (G‐6PD), and lactate dehydrogenase (LDH) is essential clinically to assess male fertility (Bull et al, 2002; Owumi, Danso, et al, 2020). During testicular energy metabolism, this array of enzymes aid in promoting normal spermatogenesis and male reproductive function (Yi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…As previously reported, total sulfhydryl group and reduced GSH was assessed by Ellman’s (1959), Arowoogun et al (2020) and Jollow et al (1974), Owumi et al (2019) method respectively. GST was assayed by Habig’s method (Habig et al, 1974) and GPx activity by the method of Rotruck et al, and as previously reported (Owumi, Danso, et al, 2020; Rotruck et al, 1973). Also, malondialdehyde (MDA)–a biomarker of cellular lipid peroxidation–was measured by the method of Okhawa (Folayan et al, 2020; Ohkawa et al, 1979), and xanthine oxidase was by the method of Bergmeyer et al (Buege & Aust, 1978; Owumi, Elebiyo, et al, 2020) and reported recently.…”

Section: Methodsmentioning

confidence: 99%

Caffeic acid mitigates aflatoxinB1‐mediated toxicity in the male rat reproductive system by modulating inflammatory and apoptotic responses, testicular function, and the redox‐regulatory systems

Owumi

Irozuru

Arunsi

et al. 2022

Journal of Food Biochemistry

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Aflatoxin B1 (AFB1) is a toxic metabolite of public health concern. The present study investigates the protective effects of caffeic acid (CA) against AFB1‐induced oxidative stress, inflammation, and apoptosis in the hypothalamus, epididymis, and testis of male rats. Five experimental rat cohorts (n = 6) were treated per os for 28 consecutive days as follows: Control (Corn oil 2 ml/kg body weight), AFB1 alone (50μg/kg), CA alone (40 mg/kg) and the co‐treated rat cohorts (AFB1: 50μg/kg + CA1: 20 or 40 mg/kg). Following sacrifice, the biomarkers of hypothalamic, epididymal, and testicular toxicities, antioxidant enzyme activities, myeloperoxidase (MPO) activity, as well as levels of nitric oxide (NO), reactive oxygen and nitrogen (RONS) species and lipid peroxidation (LPO) were analysed spectrophotometrically. Besides, the concentration of tumour necrosis factor‐alpha (TNF‐α), Bcl‐2 and Bax proteins were assessed using ELISA. Results showed that the AFB1‐induced decrease in biomarkers of testicular, epididymal and hypothalamic toxicity was significantly (p < .05) alleviated in rats coexposed to CA. Moreover, the reduction of antioxidant status and the increase in RONS and LPO were lessened (p < .05) in rats co‐treated with CA. AFB1 mediated increase in TNF‐α, Bax, NO and MPO activity were reduced (p< .05) in the hypothalamus, epididymis, and testis of rats coexposed to CA. In addition, Bcl‐2 levels were reduced in rats treated with CA dose‐dependently. Light microscopic examination showed that histopathological lesions severity induced by AFB1 were alleviated in rats coexposed to CA. Taken together, the amelioration of AFB1‐induced neuronal and reproductive toxicities by CA involves anti‐inflammatory, antioxidant, antiapoptotic mechanisms in rats. Practical applications The beneficial antioxidant effects of caffeic acid (CA) are attributed to CA delocalized aromatic rings and free electrons, easily donated to stabilize reactive oxygen species. We report in vivo findings on CA and AfB1 mediated oxidative stress and reproductive dysfunction in rats. CA conjugated esters including chlorogenic acids are widely distributed in plants, and they act as a dietary source of natural defense against infections. CA can chelate heavy metals and reduce production of damaging free radicals to cellular macromolecules. Along these lines, CA can stabilize aflatoxin B1‐epoxide as well and avert deleterious conjugates from forming with deoxyribonucleic acids. Hence CA, as a dietary phytochemical can protect against the damaging effects of toxins including aflatoxin B1 that contaminate food. CA dose‐dependently abated oxidative, inflammatory, and apoptotic stimuli, improved functional characteristics of spermatozoa and reproductive hormone levels, and prevented histological alterations in experimental rats’ hypothalamus and reproductive organs brought about by AFB1 toxicity.

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Gallic acid and omega‐3 fatty acids mitigate epididymal and testicular toxicity in manganese‐treated rats

Cited by 18 publications

References 44 publications

Apigeninidin-rich Sorghum bicolor (L. Moench) extracts suppress A549 cells proliferation and ameliorate toxicity of aflatoxin B1-mediated liver and kidney derangement in rats

Apigeninidin-rich Sorghum bicolor (L. Moench) extracts suppress A549 cells proliferation and ameliorate toxicity of aflatoxin B1-mediated liver and kidney derangement in rats

Decrease in reproductive dysfunction using aflatoxin B1 exposure: a treatment with 3‐indolepropionic acid in albino Wistar rat

Caffeic acid mitigates aflatoxinB1‐mediated toxicity in the male rat reproductive system by modulating inflammatory and apoptotic responses, testicular function, and the redox‐regulatory systems

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