Gallic acid indanone and mangiferin xanthone are strong determinants of immunosuppressive anti-tumour effects of Mangifera indica L. bark in MDA-MB231 breast cancer cells

García-Rivera, Dagmar; Delgado, René; Bougarne, Nadia; Haegeman, Guy; Berghe, Wim Vanden

doi:10.1016/j.canlet.2011.02.011

Cited by 123 publications

(98 citation statements)

References 52 publications

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“…The antitumor capability of this compound can be traced back as far as 1987 (Chattopadhyay et al, 1987). In the later stage of studies of this compound, several significant findings relevant to its anticancer properties have been reported (Chattopadhyay et al, 1987;Huang et al, 2002;Rajendran et al, 2008;Garcia-Rivera et al, 2011). As mentioned above, this compound has a mild pharmacological action that is less toxic both in vitro and in vivo (Chattopadhyay et al, 1987;Huang et al, 2001Huang et al, , 2002.…”

Section: Discussionmentioning

confidence: 96%

“…More interestingly, this flavonoid has been further shown to possess antiproliferative and antitumor growth properties, thus making it a potential anticancer agent in recent years (Huang et al, 2001;Rajendran et al, 2008;Garcia-Rivera et al, 2011). Previous in-depth studies have found that mangiferin induces apoptosis (Huang et al, 2002;Peng et al, 2004;Sarkar et al, 2004;Cheng et al, 2007;du Plessis-Stoman et al, 2011;Shoji et al, 2011) and inhibits cell cycle progression in liver cancer and leukemic cells, leading to G2/M phase arrest (Huang et al, 2002;Yao et al, 2010); however, since the compound's antitumor properties have not been studied extensively, the mechanisms of action in cell apoptosis induction and cell cycle inhibition have not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…Mangiferin is a flavonoid and it is extracted from the mango tree. Studies have found that mangiferin can inhibit the growth of liver cancer cells, colon cancer cells, leukemia K562 cells, and other tumor cells, and the inhibition of tumor cells is related to the induction of cell cycle arrest at the G2/M phase (Huang et al, 2002;Rajendran et al, 2008;Garcia-Rivera et al, 2011). On the basis of our previous studies, which showed that mangiferin inhibits the proliferation of HL-60 leukemia cells and induces apoptosis, we investigated the impact of mangiferin on the HL-60 cell cycle and the expression regulation of related genes to clarify the antileukemic mechanism of mangiferin, so as to provide experimental evidence for using mangiferin as a new leukemiatreatment drug.…”

Section: Introductionmentioning

confidence: 99%

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Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Peng¹,

Yao²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study aimed to determine the effect of mangiferin on the cell cycle in HL-60 leukemia cells and expression of the cell cycle-regulatory genes Wee1, Chk1 and CDC25C and to further investigate the molecular mechanisms of the antileukemic action of mangiferin. The inhibitory effect of mangiferin on HL-60 leukemia cell proliferation was determined by the MTT assay. The impact of mangiferin on the HL-60 cell cycle was evaluated by flow cytometry. After the cells were treated with different concentrations of mangiferin, the expression levels of Wee1, Chk1 and CDC25C mRNA were determined by RT-PCR, and Western blot was used to evaluate the expression levels of cdc25c, cyclin B1, and Akt proteins. The inhibition of HL-60 cell growth by mangiferin was dose-and time-dependent. After treatment for 24 h, cells in G2/M phase increased, and G2/M phase arrest appeared with increased mRNA expression of Wee1, Chk1 and CDC25C. Mangiferin inhibited Chk1 and cdc25c mRNA expression at high concentrations and induced Wee1 mRNA expression in a dose-dependent manner. It significantly inhibited ATR, Chk1, Wee1, Akt, and ERK1/2 phosphorylation but increased cdc2 and cyclin Z.G. Peng et al. 4990©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4989-5002 (2015) B1 phosphorylation. Furthermore, mangiferin reduced cdc25c, cyclin B1, and Akt protein levels while inducing Wee1 protein expression. It also antagonized the phosphorylation effect of vanadate on ATR, and the phosphorylation effect of EGF on Wee1. These findings indicated that mangiferin inhibits cell cycle progression through the ATR-Chk1 stress response DNA damage pathway, leading to cell cycle arrest at G2/M phase in leukemia cells.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Peng¹,

Yao²,

Yang³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

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“…Meanwhile, we found that mangiferin induced the cell apoptosis rate and increased the activation of caspase-3 and caspase-9 in U87 glioma cells. García-Rivera et al (17) indicated that the antitumor effects of mangiferin were through increased apoptosis in MDA-MB231 breast cancer cells. du Plessis-Stoman et al (18) illustrated that combination treatment with oxaliplatin and mangiferin accelerated apoptosis through inhibition of NF-κB in cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Mangiferin regulates proliferation and apoptosis in glioma cells by induction of microRNA-15b and inhibition of MMP-9 expression

et al. 2015

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Abstract. Mangiferin, a flavonoid extracted from the leaves of the Anacardiaceae plant, the mango tree, has physiological activity and pharmacological effects in many aspects. The present study aimed to clarify the effect of mangiferin on proliferation and apoptosis of glioma cells and the mechanism of these curative effects of mangiferin. In this experiment, we detected the proliferation using 3-(4,5-dimethylthylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Then, cell apoptosis of U87 glioma cells was measured with the Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, DAPI staining assay and the caspase-3 and caspase-9 activity assay kit. Next, quantitative real-time PCR and gelatin zymography were used to analyze the expression of microRNA-15b (miR-15b) and matrix metalloproteinase-9 (MMP-9), respectively. MMP-9 agonist, miR-15b mimics and anti-miR-15b mimics were added to the U87 glioma cells for elucidating the mechanisms involved in the curative effects of mangiferin. In the present study, mangiferin notably restrained the proliferation and increased the apoptosis of the U87 glioma cells. Meanwhile, mangiferin specifically promoted the expression of miR-15b and suppressed the level of MMP-9 in the U87 glioma cells. miR-15b regulated the expression of MMP-9 in the U87 glioma cells. MMP-9 agonist and antimiR-15b reduced the curative effects of mangiferin in the U87 glioma cells. In summary, mangiferin regulates proliferation and apoptosis in glioma cells by induction of miR-15b and inhibition of MMP-9 expression.

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“…Moreover, RT-qPCR confirms the reduction of IL-6 gene expression after treatment with gallic acid (Figure 3). It has been shown that gallic acid acts as an anti-tumor agent in MDA-MB 231 breast cancer cells by inhibiting the expression of genes that are involved in inflammation, metastasis, anti-apoptosis, and angiogenesis, such as TNF-α, IL-6, IL-8, COX2, and bcl2 (27). Also, numerous studies have shown that gallic acid down-regulated the expression of pro-inflammatory cytokine, such as IL-1β, TNF-α, and IL-6 in both mast cells and rheumatoid arthritis fibroblast-like synoviocytes (FLS) due to its anti-oxidant effects (28).…”

Section: Discussionmentioning

confidence: 99%

Gallic Acid Inhibits Invasion and Reduces IL-6 Gene Expression, pSTAT3, pERK1/2, and pAKT Cellular Signaling Proteins in Human Prostate Cancer DU-145 Cells

et al. 2017

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Background: Prostate cancer (PC) is a malignant disease, which is common in men. Interleukin-6 (IL-6) mediates the progression of PC through PI3K/AKT, JAK-STAT, and ERK1/2 MAPKs signaling pathways. Gallic acid, a phenolic compound, has anti-oxidant, antiproliferative, and anti-tumorigenic properties. Objectives: This study was undertaken to evaluate the effects of gallic acid on DU-145 cell viability, proliferation, invasion, IL-6 gene expression, and the cellular levels of phosphorylated STAT3, ERK1/2, and AKT signaling proteins.

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Gallic acid indanone and mangiferin xanthone are strong determinants of immunosuppressive anti-tumour effects of Mangifera indica L. bark in MDA-MB231 breast cancer cells

Cited by 123 publications

References 52 publications

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Mangiferin induces cell cycle arrest at G2/M phase through ATR-Chk1 pathway in HL-60 leukemia cells

Mangiferin regulates proliferation and apoptosis in glioma cells by induction of microRNA-15b and inhibition of MMP-9 expression

Gallic Acid Inhibits Invasion and Reduces IL-6 Gene Expression, pSTAT3, pERK1/2, and pAKT Cellular Signaling Proteins in Human Prostate Cancer DU-145 Cells

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