Gallium Nitrate Accelerates Partial Thickness Wound Repair and Alters Keratinocyte Integrin Expression to Favor a Motile Phenotype

Goncalves, John; Wasif, Nabil; Esposito, Darren; Coico, Jonathan M.; Schwartz, Brian; Higgins, Paul J.; Bockman, Richard S.; Staiano‐Coico, Lisa

doi:10.1006/jsre.2001.6347

Cited by 13 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 and 5 ). While these are not quantitative measures, it is consistent with other reports where the application of gallium salts reduced inflammation ( 62 , 64 , 65 ). Further, histopathology results coincided with the gross pathology results where reepithelization of the wound bed was positively impacted by GaCi therapy compared to that of untreated controls ( Fig.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Evaluation of Gallium Citrate Formulations against a Multidrug-Resistant Strain of Klebsiella pneumoniae in a Murine Wound Model of Infection

Thompson

Truong‐Le

Alamneh

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Skin and soft tissue infections (SSTIs) are a common occurrence in health care facilities with a heightened risk for immunocompromised patients. Klebsiella pneumoniae has been increasingly implicated as the bacterial agent responsible for SSTIs, and treatment can be challenging as more strains become multidrug resistant (MDR). Therefore, new treatments are needed to counter this bacterial pathogen. Gallium complexes exhibit antimicrobial activity and are currently being evaluated as potential treatment for bacterial infections. In this study, we tested a topical formulation containing gallium citrate (GaCi) for the treatment of wounds infected with K. pneumoniae. First, the MIC against K. pneumoniae ranged from 0.125 to 2.0 μg/ml GaCi. After this in vitro efficacy was established, two topical formulations with GaCi (0.1% [wt/vol] and 0.3% [wt/vol]) were tested in a murine wound model of MDR K. pneumoniae infection. Gross pathology and histopathology revealed K. pneumoniae-infected wounds appeared to close faster with GaCi treatment and were accompanied by reduced inflammation compared to those of untreated controls. Similarly, quantitative indications of infection remediation, such as reduced weight loss and wound area, suggested that treatment improved outcomes compared to those of untreated controls. Bacterial burdens were measured 1 and 3 days following inoculation, and a 0.5 to 1.5 log reduction of CFU was observed. Lastly, upon scanning electron microscopy analysis, GaCi treatment appeared to prevent biofilm formation on dressings compared to those of untreated controls. These results suggest that with more preclinical testing, a topical application of GaCi may be a promising alternative treatment strategy for K. pneumoniae SSTI.

show abstract

Section: Discussionsupporting

confidence: 89%

“…5D ) displayed degrees of reepithelialization coupled with noticeable granulation tissue with a small amount of overlying serocellular crust. The results provide preliminary evidence of wound healing and a reduction of inflammation, which is a property of gallium that has been previously investigated by others ( 56 , 62 ).…”

Section: Resultssupporting

confidence: 76%

Evaluation of Gallium Citrate Formulations against a Multidrug-Resistant Strain of Klebsiella pneumoniae in a Murine Wound Model of Infection

Thompson

Truong‐Le

Alamneh

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Others have shown an angiogenic effect, mainly in the modification of the hypoxia-inducible factor (HIF) pathway [318,319], in contrast with the calcium angiogenesis mechanism [148]. There is a third effect in which some metals are involved: reepithelization such as Zn 2+ and Ga 3+ [308,320]. Finally, a gas such as NO can multitask and be an important factor in the skin healing process.…”

Section: Inorganic Signallingmentioning

confidence: 99%

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Castaño

Pérez‐Amodio

Nieva

et al. 2018

Advanced Drug Delivery Reviews

168

View full text Add to dashboard Cite

Skin wound healing aims to repair and restore tissue through a multistage process that involves different cells and signalling molecules that regulate the cellular response and the dynamic remodelling of the extracellular matrix. Nowadays, several therapies that combine biomolecule signals (growth factors and cytokines) and cells are being proposed. However, a lack of reliable evidence of their efficacy, together with associated issues such as high costs, a lack of standardization, no scalable processes, and storage and regulatory issues, are hampering their application. In situ tissue regeneration appears to be a feasible strategy that uses the body's own capacity for regeneration by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the wound site to promote repair and regeneration. The aim is to engineer instructive systems to regulate the spatio-temporal delivery of proper signalling based on the biological mechanisms of the different events that occur in the host microenvironment. This review describes the current state of the different signal cues used in wound healing and skin regeneration, and their combination with biomaterial supports to create instructive microenvironments for wound healing.

show abstract

“…αvβ6 is down‐regulated in differentiated epithelia, while in injured or inflamed epithelia this integrin is up‐regulated 10–13. In addition, enhanced or de novo expression of αvβ6 expression has been observed in several epithelial malignancies 14–21 and the αvβ6 integrin may have multiple regulatory functions in oncogenesis.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the αvβ6 integrin in cervical squamous cell carcinoma is a prognostic factor for decreased survival

Hazelbag

Kenter

Gorter

et al. 2007

The Journal of Pathology

161

144

View full text Add to dashboard Cite

Cervical squamous cell carcinomas are composed histologically of tumour cell islands surrounded by varying amounts of tumour stroma, the amount and composition of which are influenced by local TGF-beta(1). TGF-beta(1) is secreted in an inactive complex with latency-associated peptide (LAP). Both LAP and the extracellular matrix (ECM) protein fibronectin are important ligands for the integrin receptor alpha v beta 6. While alpha v beta 6 is only weakly expressed by normal epithelia, it is up-regulated in different carcinomas where it generally reflects a more aggressive phenotype. In cervical cancer, the expression of alpha v beta 6 has not thus far been investigated. Given the ability of alpha v beta 6 both to activate TGF-beta(1) and to interact with fibronectin, we studied correlations between the expression of these components and disease parameters in a large cohort of cervical cancer specimens. We analysed alpha v beta 6 expression using immunohistochemistry in primary cervical squamous carcinomas of FIGO stage IA to IIB patients and correlated the findings with formerly investigated fibronectin and TGF-beta(1) expression and clinico-pathological parameters. alpha v beta 6 expression was also examined in cervical intra-epithelial neoplasia (CIN) and lymph node metastases. alpha v beta 6 was only weakly expressed in normal epithelium but clearly up-regulated in CIN lesions. In carcinomas, strong expression of alpha v beta 6 in tumour cells correlated with different clinico-pathological parameters and with worse overall and disease-free survival. Furthermore, alpha v beta 6 expression correlated positively with TGF-beta(1) mRNA expression as well as with fibronectin expression. Overexpression of alpha v beta 6 in cervical squamous carcinomas is an unfavourable prognostic factor. This might reflect an increased capacity of alpha v beta 6-expressing tumour cells to migrate in a fibronectin-rich ECM and/or to activate TGF-beta(1) at the tumour/stroma interface, both of which processes may contribute to cervical cancer progression.

show abstract

Gallium Nitrate Accelerates Partial Thickness Wound Repair and Alters Keratinocyte Integrin Expression to Favor a Motile Phenotype

Cited by 13 publications

References 32 publications

Evaluation of Gallium Citrate Formulations against a Multidrug-Resistant Strain of Klebsiella pneumoniae in a Murine Wound Model of Infection

Evaluation of Gallium Citrate Formulations against a Multidrug-Resistant Strain of Klebsiella pneumoniae in a Murine Wound Model of Infection

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Overexpression of the αvβ6 integrin in cervical squamous cell carcinoma is a prognostic factor for decreased survival

Contact Info

Product

Resources

About