Gallium Phosphide Nanowires as a Substrate for Cultured Neurons

Hällström, Waldemar; Mårtensson, Thomas; Prinz, Christelle; Gustavsson, Per; Montelius, Lars; Samuelson, Lars; Kanje, Martin

doi:10.1021/nl070728e

Cited by 184 publications

(193 citation statements)

References 27 publications

Supporting

Mentioning

185

Contrasting

Order By: Relevance

“…Building on the previous observation that vertically grown NWs can support cell culture (15)(16)(17)(18)(19), we show that vertical NW substrates can serve as a universal, scalable, and highly efficient modality for introducing DNAs, RNAs, peptides, proteins, and small molecules into both immortalized and primary cell types, without chemical modification or viral packaging. This simplicity and generality, which stem from the direct physical access to the cell's interior afforded by NW penetration (15)(16)(17)(18)(19), make NW-based delivery distinct from chemical and viral methods that are widely employed (1)(2)(3)(4)(5)(6). Furthermore, this NW-based system is fully compatible with standard microarray printing techniques, allowing large collections of biomolecules to be delivered, or even codelivered, into live cells in a parallel and miniaturized fashion.…”

mentioning

confidence: 52%

“…Previous studies have shown that vertical NWs spontaneously penetrate cells' membranes when cells are placed on top of the NWs (15)(16)(17)(18)(19). To visualize this penetration process, DiDmembrane-labeled HeLa cells (Magenta) were seeded on a bed of Si NWs whose gold tips had been prelabeled with This article contains supporting information online at www.pnas.org/cgi/content/full/ 0909350107/DCSupplemental.…”

Section: Resultsmentioning

confidence: 99%

“…Vertical nanowire (NW) substrates can satisfy these stringent requirements because the NWs enable direct physical access to the cells' interiors (15)(16)(17)(18)(19). Here, we report an experimental platform based on vertical silicon (Si) NWs that can deliver virtually any type of molecule into a wide variety of cells in a format compatible with microarray technology and live-cell imaging.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

Shalek

Robinson

Karp

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

534

571

View full text Add to dashboard Cite

A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies on the ability of the silicon nanowires to penetrate a cell's membrane and subsequently release surface-bound molecules directly into the cell's cytosol, thus allowing highly efficient delivery of biomolecules without chemical modification or viral packaging. This modality enables one to assess the phenotypic consequences of introducing a broad range of biological effectors (DNAs, RNAs, peptides, proteins, and small molecules) into almost any cell type. We show that this platform can be used to guide neuronal progenitor growth with small molecules, knock down transcript levels by delivering siRNAs, inhibit apoptosis using peptides, and introduce targeted proteins to specific organelles. We further demonstrate codelivery of siRNAs and proteins on a single substrate in a microarray format, highlighting this technology's potential as a robust, monolithic platform for high-throughput, miniaturized bioassays. intracellular delivery | microarray | high-throughput bioassay | nanobiotechnology

show abstract

mentioning

confidence: 52%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

Shalek

Robinson

Karp

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

534

571

View full text Add to dashboard Cite

show abstract

“…Recently, Shalek et al reported the use of vertical silicon nanowires to deliver biomolecules such as proteins and DNA plasmids into living cells that grew attached to the nanowires (9). Together with other works that show vertical nanowires can support cell culture (10)(11)(12)(13)(14), these observations suggest a new and exciting possibility of using vertical nanowires as a universal platform to probe intracellular molecular events.…”

mentioning

confidence: 86%

Vertical nanopillars for highly localized fluorescence imaging

Xie

Hanson

Cui

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

101

View full text Add to dashboard Cite

Observing individual molecules in a complex environment by fluorescence microscopy is becoming increasingly important in biological and medical research, for which critical reduction of observation volume is required. Here, we demonstrate the use of vertically aligned silicon dioxide nanopillars to achieve below-the-diffraction-limit observation volume in vitro and inside live cells. With a diameter much smaller than the wavelength of visible light, a transparent silicon dioxide nanopillar embedded in a nontransparent substrate restricts the propagation of light and affords evanescence wave excitation along its vertical surface. This effect creates highly confined illumination volume that selectively excites fluorescence molecules in the vicinity of the nanopillar. We show that this nanopillar illumination can be used for in vitro single-molecule detection at high fluorophore concentrations. In addition, we demonstrate that vertical nanopillars interface tightly with live cells and function as highly localized light sources inside the cell. Furthermore, specific chemical modification of the nanopillar surface makes it possible to locally recruit proteins of interest and simultaneously observe their behavior within the complex, crowded environment of the cell.

show abstract

“…26,[31][32][33][34][35] Second, cell proliferation/adhesion are not seriously affected by surficial compositions, other than surficial topographies. [67][68][69] In addition, histological inflammation and infection do not easily occur with sterilized extraneous objects without the involvement of germs, viruses, or macroscopic stabs.…”

Section: Apl Mater 5 074102 (2017)mentioning

confidence: 99%

Comprehensive biocompatibility of nontoxic and high-output flexible energy harvester using lead-free piezoceramic thin film

et al. 2017

View full text Add to dashboard Cite

Flexible piezoelectric energy harvesters have been regarded as an overarching candidate for achieving self-powered electronic systems for environmental sensors and biomedical devices using the self-sufficient electrical energy. In this research, we realize a flexible high-output and lead-free piezoelectric energy harvester by using the aerosol deposition method and the laser lift-off process. We also investigated the comprehensive biocompatibility of the lead-free piezoceramic device using ex-vivo ionic elusion and in vivo bioimplantation, as well as in vitro cell proliferation and histologic inspection. The fabricated LiNbO3-doped (K,Na)NbO3 (KNN) thin film-based flexible energy harvester exhibited an outstanding piezoresponse, and average output performance of an open-circuit voltage of ∼130 V and a short-circuit current of ∼1.3 μA under normal bending and release deformation, which is the best record among previously reported flexible lead-free piezoelectric energy harvesters. Although both the KNN and Pb(Zr,Ti)O3 (PZT) devices showed short-term biocompatibility in cellular and histological studies, excessive Pb toxic ions were eluted from the PZT in human serum and tap water. Moreover, the KNN-based flexible energy harvester was implanted into a porcine chest and generated up to ∼5 V and 700 nA from the heartbeat motion, comparable to the output of previously reported lead-based flexible energy harvesters. This work can compellingly serve to advance the development of piezoelectric energy harvesting for actual and practical biocompatible self-powered biomedical applications beyond restrictions of lead-based materials in long-term physiological and clinical aspects.

show abstract

Gallium Phosphide Nanowires as a Substrate for Cultured Neurons

Cited by 184 publications

References 27 publications

Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

Vertical nanopillars for highly localized fluorescence imaging

Comprehensive biocompatibility of nontoxic and high-output flexible energy harvester using lead-free piezoceramic thin film

Contact Info

Product

Resources

About