Gallotannin Isolated from Euphorbia Species, 1,2,6-Tri-O-galloyl-.BETA.-D-allose, Decreases Nitric Oxide Production through Inhibition of Nuclear Factor-.KAPPA.&gt;B and Downstream Inducible Nitric Oxide Synthase Expression in Macrophages

Kim, Mi-Sun; Park, Seung-Bin; Suk, Kyoungho; Kim, In Kyeom; Kim, Sang-Yong; Kim, Jeong Ah; Lee, Seung Ho; Kim, Sang-Hyun

doi:10.1248/bpb.32.1053

Cited by 37 publications

(21 citation statements)

References 33 publications

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“…Day zero on the graph is day 7 after tumor injection. *Denotes significant differences (p < 0.05) with respect to control (days [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], † significant with respect to prevention group at day 14. (B) Real time pCR analysis of TNFα, IL-1α and IL6 in the tumor tissue sections of control and GT-treated mice.…”

Section: Discussionmentioning

confidence: 99%

Gallotannin inhibits NFĸB signaling and growth of human colon cancer xenografts

Al-Halabi¹,

Chedid²,

Merhi³

et al. 2011

Cancer Biology & Therapy

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Section: Discussionmentioning

confidence: 99%

Gallotannin inhibits NFĸB signaling and growth of human colon cancer xenografts

Al-Halabi¹,

Chedid²,

Merhi³

et al. 2011

Cancer Biology & Therapy

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“…Currently, there is no direct evidence that antioxidant treatments decrease NO in nemertean oocytes by either downregulating NOS activity or scavenging NO, as demonstrated for somatic cells (Bergamini et al 2001, Linares et al 2008, Kim et al 2009, Harput et al 2011. Moreover, even if the antioxidants had been shown to reduce intraoocytic NO, such findings would not preclude the possibility that these broadly acting drugs block GVBD via effects on other targets, including ROS (Nakagawa et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Such oocytes were then transferred to SW solutions of antioxidants (ascorbic acid, butylated hydroxyanisole (BHA), gallotannin, N-acetylcysteine (NAC), or tempol), which were chosen in order to compare with previous studies that subjected mammalian oocytes and somatic cells to these and related compounds (Sun et al 2000, Ali et al 2003, Tao et al 2004a, Dumollard et al 2007, Kim et al 2009). In such tests, SW-induced GVBD in nemerteans was blocked in a dose-dependent fashion, and O50% inhibition occurred at drug concentrations that were comparable with those used on other oocytes (Sun et al 2000, Ali et al 2003, Tao et al 2004aFig.…”

Section: Blockage Of Sw-induced Gvbd By Antioxidantsmentioning

confidence: 99%

“…Since each of the tested antioxidants can lower NO levels in somatic cells (Bergamini et al 2001, Linares et al 2008, Kim et al 2009, Harput et al 2011 Figure 1 (A) Antioxidants (aa, ascorbic acid; BHA, butylated hydroxyanisole; gallo, gallotannin; NAC, N-acetylcysteine; tem, tempol) block seawater (SW)-induced GVBD in a dose-dependent manner. (B and C) Conversely, such blockage is reversed after coadding 5 mM of the cAMP elevator forskolin (fors) to 200 mM aa, 500 mM BHA, 100 mM gallo, 150 mM NAC, or 150 mM tem.…”

Section: Role Of No During Gvbdmentioning

confidence: 99%

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Inhibition of germinal vesicle breakdown by antioxidants and the roles of signaling pathways related to nitric oxide and cGMP during meiotic resumption in oocytes of a marine worm

Stricker

2012

Reproduction

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In mammalian oocytes, cAMP elevations prevent the resumption of meiotic maturation and thereby block nuclear disassembly (germinal vesicle breakdown (GVBD)), whereas nitric oxide (NO) and its downstream mediator cGMP can either inhibit or induce GVBD. Alternatively, some invertebrate oocytes use cAMP to stimulate, rather than inhibit, GVBD, and in such cases, the effects of NO/cGMP signaling on GVBD remain unknown. Moreover, potential interactions between NO/cGMP and AMP-activated kinase (AMPK) have not been assessed during GVBD. Thus, this study analyzed intraoocytic signaling pathways related to NO/cGMP in a marine nemertean worm that uses cAMP to induce GVBD. For such tests, follicle-free nemertean oocytes were stimulated to mature by seawater (SW) and cAMP elevators. Based on immunoblots and NO assays of maturing oocytes, SW triggered AMPK deactivation, NO synthase (NOS) phosphorylation, and an NO elevation. Accordingly, SW-induced GVBD was blocked by treatments involving the AMPK agonist AICAR, antioxidants, the NO scavenger carboxy-PTIO, NOS inhibitors, and cGMP antagonists that target the NO-stimulated enzyme, soluble guanylate cyclase (sGC). Conversely, SW solutions combining NO/cGMP antagonists with a cAMP elevator restored GVBD. Similarly, AICAR plus a cAMP-elevating drug reestablished GVBD while deactivating AMPK and phosphorylating NOS. Furthermore, sGC stimulators and 8-Br-cGMP triggered GVBD. Such novel results indicate that NO/cGMP signaling can upregulate SW-induced GVBD and that cAMP-elevating drugs restore GVBD by overriding the inhibition of various NO/cGMP downregulators, including AMPK. Moreover, considering the opposite effects of intraoocytic cAMP in nemerteans vs mammals, these data coincide with previous reports that NO/cGMP signaling blocks GVBD in rats.

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“…22 Briefly, 50 lL of culture supernatant was mixed with an equal volume of Griess reagent and incubated at room temperature for 10 min. The absorbance at 540 nm was read using a Power Wavex Microplate Scanning spectrophotometer (Bio-Tek Instrument, Winooski, VT, USA).…”

Section: Nitric Oxidementioning

confidence: 99%

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

Kim

Tang

Cha

et al. 2015

Journal of Medicinal Food

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The present study aimed to examine the anti-inflammatory effects and potential mechanism of action of Artemisia asiatica Nakai (A. asiatica Nakai) extract in activated murine macrophages. A. asiatica Nakai extract showed dosedependent suppression of lipopolysaccharide (LPS)-induced nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 activity. It also showed dose-dependent inhibition of nuclear factor-jB (NF-jB) translocation from the cytosol to the nucleus and as an inhibitor of NF-jB-alpha phosphorylation. The extract's inhibitory effects were found to be mediated through NF-jB inhibition and phosphorylation of extracellular signal-regulated kinase 1/2 and p38 in LPS-stimulated J774A.1 murine macrophages, suggesting a potential mechanism for the anti-inflammatory activity of A. asiatica Nakai. To our knowledge, this is the first report of the anti-inflammatory effects of A. asiatica Nakai on J774A.1 murine macrophages; these results may help develop functional foods possessing an anti-inflammatory activity.

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Gallotannin Isolated from Euphorbia Species, 1,2,6-Tri-O-galloyl-.BETA.-D-allose, Decreases Nitric Oxide Production through Inhibition of Nuclear Factor-.KAPPA.>B and Downstream Inducible Nitric Oxide Synthase Expression in Macrophages

Cited by 37 publications

References 33 publications

Gallotannin inhibits NFĸB signaling and growth of human colon cancer xenografts

Gallotannin inhibits NFĸB signaling and growth of human colon cancer xenografts

Inhibition of germinal vesicle breakdown by antioxidants and the roles of signaling pathways related to nitric oxide and cGMP during meiotic resumption in oocytes of a marine worm

Artemisia asiaticaNakai Attenuates the Expression of Proinflammatory Mediators in Stimulated Macrophages Through Modulation of Nuclear Factor-κB and Mitogen-Activated Protein Kinase Pathways

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