Galloyl flavonoids from Acacia farnesiana pods possess potent anthelmintic activity against Haemonchus contortus eggs and infective larvae

Zarza-Albarrán, M.A.; Olmedo-Juárez, Agustín; Rubio, Rolando Rojo; Gives, Pedro Mendoza de; González-Cortazar, Manasés; Tapia‐Maruri, Daniel; Mondragón-Ancelmo, Jaime; García-Hernández, César; Blé-González, Ever A.; Zamilpa, Alejandro

doi:10.1016/j.jep.2019.112402

Cited by 35 publications

(24 citation statements)

References 38 publications

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“…Additionally, 50 µL of the corresponding fractions, sub-fractions, compounds, and controls were added to each well. Then, the plates were incubated at room temperature (18-25 • C) for 72 h [21]. After incubation, ten 10-µL aliquots were removed from each well and deposited onto a slide for microscopic examination.…”

Section: Assessment Of Larval Mortality By a Bio-guided Assaymentioning

confidence: 99%

Isorhamnetin: A Nematocidal Flavonoid from Prosopis laevigata Leaves Against Haemonchus contortus Eggs and Larvae

Delgado-Núñez

Zamilpa²,

González-Cortazar³

et al. 2020

Biomolecules

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Haemonchus contortus affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. Prosopis laevigata is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary study where a P. laevigata hydroalcoholic extract (Pl-hae) showed anthelmintic activity (aa) against H. contortus. Searching for bioactive compounds (bac) with high aa, the Pl-hae was fractioned obtaining an aqueous (Aq-F) and an ethyl acetate fraction (EtAc-F), and a flavonoid with aa identified as isorhamnetin was obtained from EtAc-F. Both fractions were in vitro assessed by the egg hatch test (eht) and larval mortality (lm) assays. The bac obtained from EtAc-F were characterised by NMR analysis. The highest aa were recorded with EtAc-F, resulting in 100% eht and 80.45% lm at 0.75 and 30 mg/mL, respectively. Alterations in eggs and larvae attributed to isorhamnetin were recorded by environmental scanning electron microscopy, confocal laser scanning and by high-resolution digital-coupled camera. This flavonoid caused 100% eht at 0.07 mg/mL after 48 h and 100% lm at 7.5 mg/mL after 72 h exposure. Isorhamnetin has promising potential as an anthelmintic against sheep haemonchosis.

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Section: Assessment Of Larval Mortality By a Bio-guided Assaymentioning

confidence: 99%

Isorhamnetin: A Nematocidal Flavonoid from Prosopis laevigata Leaves Against Haemonchus contortus Eggs and Larvae

Delgado-Núñez

Zamilpa²,

González-Cortazar³

et al. 2020

Biomolecules

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“…Undoubtedly the more-studied PSC in terms of ruminant nutrition and AH activity are the CT [ 13 , 27 , 28 , 29 , 30 ]. Nonetheless, it is known that other PSC such as flavonoids, saponins, terpenes, alkaloids, hydrolysable tannins, among others, could influence the physiology of both ruminants and nematodes [ 34 , 190 , 191 , 192 , 193 , 194 , 195 , 196 ]. Hence, considering the plethora of plant species and PSC present in the LDF, we have a wide avenue of future research.…”

Section: Final Remarks and Implicationsmentioning

confidence: 99%

Small Ruminant Production Based on Rangelands to Optimize Animal Nutrition and Health: Building an Interdisciplinary Approach to Evaluate Nutraceutical Plants

Torres-Fajardo

González-Pech

Sandoval-Castro

et al. 2020

Animals

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The plant kingdom can influence the productivity and health of herbivores at different levels. However, demonstrating this process in a scientific manner entails substantial endeavors from different disciplines. In the present review, we will describe the features of a native vegetation system traditionally used by small ruminants and use its particularities to build an interdisciplinary approach to evaluate the nutraceutical properties of plants. Initially, we will establish the context of the low deciduous forest (LDF), considering some botanical and nutritional aspects, as well as the presence of plant secondary compounds (PSC) and gastrointestinal nematodes (GIN). Furthermore, we will focus on coevolutionary aspects that undoubtedly shaped the plants–nutrients–PSC–GIN–herbivore relationship. In addition, the concept of nutraceutical will be discussed to provide clarity and aspects to be considered for their evaluation. Then, ethological, agronomical, nutritional, PSC, parasitological and animal species issues are deepened placing emphasis on methodological approaches. Special focus is given to condensed tannins, as they are the fourth largest group of PSCs and the most studied in livestock sciences. Validation of the nutraceutical properties of plants from native vegetation systems should be seen as a process derived from many scientific disciplines that feed into each other in a cyclic manner.

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“…Several studies performed in different countries have reported different plant materials with nutraceutical properties affecting GIN of ruminants [59][60][61][62][63]. Most of those studies used in vitro screening protocols to investigate different plant extracts or to identify bioactive molecules/metabolites with AH activity against ruminant parasitic nematodes [64,65]. The protocols employed provide a useful guide to develop protocols to test EM and their derivatives.…”

Section: Nutraceutical Materials As Tools For the Sustainablementioning

confidence: 99%

The Possible Biotechnological Use of Edible Mushroom Bioproducts for Controlling Plant and Animal Parasitic Nematodes

Castañeda-Ramírez

Torres-Acosta

Sánchez

et al. 2020

BioMed Research International

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The present paper reviewed publications on the nematocidal activity of edible mushrooms (EM) and their potential use as sustainable tools for the control of parasitic nematodes affecting agriculture and livestock industry. Nematodes are organisms living in the soil and animals’ guts where they may live as parasites severely affecting economically important crops and farm animals, thus causing economic losses to worldwide agriculture. Traditionally, parasitic nematodes have been controlled using commercial pesticides and anthelmintic (AH) drugs. Over the years, nematodes developed resistance to the AH drugs, reducing the usefulness of many commercial drugs. Also, the use of pesticides/anthelmintic drugs to control nematodes can have important negative impacts on the environment. Different EM have been not only used as food but also studied as alternative methods for controlling several diseases including parasitic nematodes. The present paper reviewed publications from the last decades about the nematocidal activity of EM and assessed their potential use as sustainable tools for the control of nematodes affecting agriculture and livestock industry. A reduced number of reports on the effect of EM against nematodes were found, and an even smaller number of reports regarding the potential AH activity of chemical compounds isolated from EM products were found. However, those studies have produced promising results that certainly deserve further investigation. It is concluded that EM, their fractions and extracts, and some compounds contained in them may have biotechnological application for the control of animal and plant parasitic nematodes.

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Galloyl flavonoids from Acacia farnesiana pods possess potent anthelmintic activity against Haemonchus contortus eggs and infective larvae

Cited by 35 publications

References 38 publications

Isorhamnetin: A Nematocidal Flavonoid from Prosopis laevigata Leaves Against Haemonchus contortus Eggs and Larvae

Isorhamnetin: A Nematocidal Flavonoid from Prosopis laevigata Leaves Against Haemonchus contortus Eggs and Larvae

Small Ruminant Production Based on Rangelands to Optimize Animal Nutrition and Health: Building an Interdisciplinary Approach to Evaluate Nutraceutical Plants

The Possible Biotechnological Use of Edible Mushroom Bioproducts for Controlling Plant and Animal Parasitic Nematodes

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