Gallus gallus domesticus: immune system and its potential for generationof immunobiologics

Júnior, Álvaro Ferreira; Santos, Jandra Pacheco dos; Sousa, Iara de Oliveira; Martin, I.M.C.; Alves, Endrigo Gabellini Leonel; Rosado, Isabel Rodrigues

doi:10.1590/0103-8478cr20180250

Cited by 11 publications

(10 citation statements)

References 57 publications

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“…The WD method produces the greatest yield (96%) using a salt precipitation cryo-ethanol treatment, followed by the release of the IgY powder by heating, thereby stabilizing the antibody molecules ( 16 ). Hen egg yolk regularly has 8–20 mg/ml of IgY ( 11 ). Absorbance at 280 nm wavelength using NanoDrop One apparatus and Pierce TM Chicken IgY Purification Kit (Thermo Fisher Scientific TM , New York, NY, USA) were used to determine the IgY concentration.…”

Section: Methodsmentioning

confidence: 99%

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Impact of Dietary Egg Yolk IgY Powder on Behavior, Meat Quality, Physiology, and Intestinal Escherichia coli Colonization of Broiler Chicks

Rehan

Abou-Elnaga

et al. 2022

Front. Vet. Sci.

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The current study investigated the impact of different concentrations of purified egg yolk immunoglobulin Y (IgY) supplemental food on the growth performance, behaviors, cecal contents of Escherichia coli, and the meat quality of broiler chicks. Four dietary groups were given to 180 female Ross broiler chicks at random (n = 45 for each). The control group was fed a standard diet only, whereas the other three experimental groups were fed the same basic diet supplemented with 1,500, 3,000, and 4,000 μg/ml IgY for a duration of 42 days. Significant greater behavioral activities, including, feeding, drinking, and dust bathing (p < 0.05), in the birds fed 4,000 μg/ml of IgY compared to the control group were observed. Greater weight gains of the crop, proventriculus, gizzard, and intestine (p < 0.05) were observed for broiler chicks fed 4,000 μg/ml of IgY when compared to the control group. After 3 weeks of feeding, the groups fed 3,000 and 4,000 μg/ml IgY had significant lower E. coli counts in the muscle and cecal contents (p < 0.05) when compared to the control group. Moreover, dietary supplementation with 4,000 μg/ml IgY in the third week and 3,000 μg/ml IgY in the sixth week resulted in greater weight gain (p < 0.01) when compared to the control group. Also, at week 3, chicks fed 4,000 μg/ml of IgY had a lower feed conversion ratio (FCR) when compared to the control group (p < 0.05). At week 6, chicks fed 3,000 μg/ml of IgY had lower FCR than the control (p < 0.05). The circulating heterophile/lymphocyte ratio was simply altered in birds fed variable IgY concentrations (1,500, 3,000, and 4,000 μg/ml), with no significant differences compared to the control group due to the individual resistance of each bird to physiological stress. The addition of 4,000 μg/ml IgY to the diet enhanced the nutritive value of meat, including protein, fat, and ash content (p < 0.05). Our study concluded that dietary supplementation of 3,000 and/or 4,000 μg/ml IgY improved the growth rates, behavioral activities, intestinal health indices, and meat quality of broiler chicks.

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Section: Methodsmentioning

confidence: 99%

“…On the day 7 after hatching, the IgY is reduced as soon as the bird starts developing antibodies ( 10 ). Hen egg yolk has 8–20 mg/ml of IgY ( 11 ). Recently, we have revealed that broilers fed a novel combination of IgY and probiotics improved their behavioral activities and immunity responses ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of Dietary Egg Yolk IgY Powder on Behavior, Meat Quality, Physiology, and Intestinal Escherichia coli Colonization of Broiler Chicks

Rehan

Abou-Elnaga

et al. 2022

Front. Vet. Sci.

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“…En base a este principio inmunológico y considerando que la yema de huevo es una fuente económica y accesible para obtener anticuerpos, la tecnología IgY desarrolla una metodología práctica, repetible, segura y de bajo costo para la producción de IgY 30 . Se comienza por inmunizar a las gallinas (por vía subcutánea, intramuscular o intradérmica), con el fin de desarrollar una respuesta inmunológica adaptativa humoral 40 . Para ello se pueden utilizar diferentes tipos de antígenos como patógenos inactivados, proteínas recombinantes o proteínas obtenidas de ADN plasmídico 41 .…”

Section: Opsonizaciónunclassified

Tecnología IgY: Estrategia en el tratamiento de enfermedades infecciosas humanas

Tipantiza

Nathaly

2021

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La aparición de microorganismos resistentes a antibióticos, el descubrimiento de nuevos agentes patógenos con potencial pandémico y el aumento de una población inmunocomprometida han dejado casi obsoleta la terapia antimicrobiana, terapia comúnmente usada para tratar enfermedades infecciosas. Por otro lado, las investigaciones acerca del uso del anticuerpo IgY para desarrollar inmunidad pasiva han demostrado el potencial que tiene la tecnología IgY para tratar enfermedades infecciosas víricas y bacterianas. Donde los anticuerpos IgY de aves se destacan por su alta especificidad, rendimiento y escalabilidad de producción a menor costo, con relación a los anticuerpos IgG de mamíferos. El objetivo de esta revisión es determinar la importancia del uso de los anticuerpos IgY como tratamiento terapéutico y profiláctico frente a los patógenos causantes de infecciones virales y bacterianas en humanos, mediante la recopilación de ensayos clínicos, productos comerciales y patentes registradas en el período de 2010-2021. Finalmente, con este estudio se estableció que la tecnología IgY es una herramienta biotecnológica versátil y eficaz para tratar y prevenir enfermedades infecciosas, al reducir los síntomas y la carga del patógeno.

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“…One of the main features of peripheral organs of the immune system is their location. They are always localized in areas of possible penetration of antigens into an animal's organism (Nasrin et al, 2012;Doneley, 2016;Junior et al, 2018;Nochi, et al, 2018). It is well known, that most antigens penetrate into an animal's body through the digestive system.…”

Section: Introductionmentioning

confidence: 99%

The lipid metabolism in carp during invasion by the tapeworn Bothriocephalus acheilognathi

Yuskiv

2020

Regul. Mech. Biosyst.

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The changes in total lipids, their fatty acid composition and the ratio of individual classes were established in tissues of the intestine, hepatopancreas and skeletal muscles of carp (Cyprinus carpio Linnaeus, 1758), with body weight 14.5–20.5 g, at different rates of invasion by Bothriocephalus acheilognathi (Yamaguti, 1934) helminth, which belongs to the family Bothriocephalidae, of the Pseudophyllidae order, of the Cestoda class. The examined carp was divided into three groups: first group of fish was free from intestinal helminths of B. acheilognathi (control); second group of fish was weakly infected with helminths (intensity of invasion is 1–3 helminths per fish); the third group of fish was highly infected (the invasion intensity is 4 worms and more per fish). Our results showed that fish infected with helminth B. acheilognathi compared to uninfected fish had reduced total lipid level in the gut due to phospholipids, triacylglycerols, and also lipids were characterized by lower content of linoleic, linolenic, arachidonic, penta- and hexanoic fatty acids; decrease in the level of unsaturated and increase in the content of saturated fatty acids, which leads to an increase of the saturation factor. During the infection of carp with B. acheilognathi in the hepatopancreas, the content of total lipids, structural lipids – phospholipids and reserve energy sources – triacylglycerols is probably reduced, and lipids are characterized by a high content of saturated fatty acids (С14:0, С16:0, С18:0) and lower content of unsaturated: arachidonic (С20:4), linolenic (С18:3) and linoleic acid (С18:2), which is associated with a decrease in the source for the synthesis of a number of polyunsaturated fatty acids, especially docosahexaenoic (С22:6). The total content of lipids, triacylglycerols, free fatty acids and phospholipids in skeletal muscle of carp during the Bothriocephalus invasion decreased and the content of free cholesterol, mono- and triacylglycerols increased. Helminth B. acheilognathi has the effect of reducing the total lipids of the skeletal muscle content of С18-, С20-, С22-polyunsaturated fatty acids and increasing the content of saturated (С14:0, С16:0, С18:0) and monounsaturated (С16:1, С18:1) fatty acids. The obtained results prove that the parasite B. acheilognathi in the intestine of the carp significantly affects the nutrition processes of the host depending on the intensity of the damage by helminths, which is accompanied by impaired lipid metabolism.

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Gallus gallus domesticus: immune system and its potential for generationof immunobiologics

Cited by 11 publications

References 57 publications

Impact of Dietary Egg Yolk IgY Powder on Behavior, Meat Quality, Physiology, and Intestinal Escherichia coli Colonization of Broiler Chicks

Impact of Dietary Egg Yolk IgY Powder on Behavior, Meat Quality, Physiology, and Intestinal Escherichia coli Colonization of Broiler Chicks

Tecnología IgY: Estrategia en el tratamiento de enfermedades infecciosas humanas

The lipid metabolism in carp during invasion by the tapeworn Bothriocephalus acheilognathi

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