Gambaran Histopatologi Toksisitas Ginjal Tikus Jantan (Rattus norvegicus) yang diberi Sirup Umbi Yakon (Smallanthus sonchifolius)

Abstract

Penelitian ini bertujuan untuk menganalisis gambaran histopatologi ginjal tikus jantan setelah pemberian sirup umbi yakon. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terbagi dalam 3 kelompok yakni KK sebagai kelompok kontrol (akuades), KP sebagai Kelompok perlakuan: KP1 (Sirup umbi yakon tanpa inhibitor) dan KP2 (Sirup umbi yakon dengan inhibitor) masing-masing tiga ulangan. Parameter: degenerasi sel dan nekrosis sel pada histologi ginjal yang diamati menggunakan mikroskop. Analisis data menggunakan uji non-parametrik Kruskall-Wallis dan Uji Mann-Whitney yang diolah menggunakan sofware SPSS 16. Berdasarkan hasil histologi ginjal pemberian sirup umbi yakon dengan inhibitor menunjukkan presentase kerusakan lebih berat dibandingkan pemberian sirup umbi yakon tanpa inhibitor dengan parameter kerusakan: degenerasi hidrofik dan juga hyalin sebesar 27,7±11,8 pada tingkat kerusakan ringan (+) yakni kerusakan sel mencapai 25% dan sel yang mengalami nekrosis sel 22,1±9,6 pada tingkat kerusakan sedang (++) yakni kerusakan sel mencapai 50%. Berdasarkan skoring hasil uji Kruskall-Wallis menunjukkan adanya pengaruh pemberian sirup umbi yakon terhadap kerusakan histopatologi ginjal yang sangat segnifikan (P<0,000) dan hasil uji Man-Whitney menunjukkan pemberian sirup umbi yakon dengan inibitor mengalami kerusakan sel paling tinggi dengan skor rata rata 1,96±0,12 (P<0,000). Simpulan penelitian ini pemberian sirup umbi yakon dapat menimbulkan efek toksik pada gambaran histopatologi ginjal tikus jantan.

Toxicity Test on the Combination of Caesalpinia sappan and Zingiber officinale in Rattus norvegicus Induced by Complete Freund's Adjuvant

“…Observation of the liver, kidney, and spleen tissue was performed using a light microscope with the entire field of view and a magnification of 40X and 400X. The observations showed cell degeneration and necrosis in the kidneys, hydropic and fatty degeneration and necrosis in the hepatocytes of the liver, and bleeding and necrosis in the spleen (Fitmawati et al 2018, Hidayati et al 2018, Jannah & Budijastuti 2022.…”

Antihyperglycemic Activity of Red Fruit Oil (Pandanus conoideus Lam) on Improving Kidney Function in STZ- NA-Induced Nephropathy Rats

“…Histopathological changes were evaluated according to the Arshad system by classifying histopathological changes in rat kidneys as no change (0), mild damage (1), moderate damage (2), and severe damage (3). Percentage of mild damage (changes ˂30%), moderate damage (changes ˂50%), and severe damage (changes ˃50%) (Jannah & Budijastuti, 2022). The results of the histopathological observations and percentage of kidney damage are shown in Figure 4 and Damage to the kidneys can be caused by several factors, including toxic substances that enter the body and interfere with kidney function.…”

“…Changes occur in the nucleus in the form of loss of chromatin images: the nucleus appears denser and becomes wrinkled, no longer vascular, the color becomes dark (picnosis), the nucleus is divided into torn fragments (cariorexis), and the nucleus appears pale or unreal because it no longer takes up much color (karyolysis). no longer takes up much color (karyolysis) (Jannah & Budijastuti, 2022).…”

In Vitro and In Vivo Nephrotoxicity Evaluation of the Methanol Extract of Ficus deltoidea Jack Leaf

“…17 As per the principle of drug excretion, when the amount of a drug administered surpasses the normal dosage, it can become a nephrotoxic chemical. 18 High blood flow to the kidneys causes various drugs and chemicals in the systemic circulation to be transported in significant quantities to the kidneys. The accumulation of these toxic substances will result in kidney damage.…”