In the process of successful establishment and development of service ecosystem organization, manufacturing companies not only pay attention to their own resources, business models and competitive advantages, but also pay attention to the positioning in the manufacturing service ecosystem and the symbiosis with other members of the system to achieve complementarity and symbiotic development of resources among members. Based on this, we take the manufacturing service ecosystem in the transformation and upgrading of the manufacturing enterprises in China as the research object, and comprehensively uses the theory of evolutionary game and ecosystem to study the symbiotic evolutionary stability of the manufacturing service ecosystem. Through simulation, we find that integrator is more sensitive to the change of rights than supplier. Our conclusions have certain reference for manufacturing companies to improve the collaborative operation efficiency of manufacturing service ecosystem.