Gamete fusion is facilitated by two sperm cell-expressed DUF679 membrane proteins

Cyprys, Philipp; Lindemeier, Maria; Sprunck, Stefanie

doi:10.1038/s41477-019-0382-3

Cited by 92 publications

(92 citation statements)

References 30 publications

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“…Whether ROS is involved in the thiol-based redox modification of these CRP proteins and these modifications function in pollen tube guidance growth await further study. In addition, small cysteine-rich EGG CELL 1 proteins secreted from the egg cell in Arabidopsis are necessary for sperm cell activation in malefemale gamete interactions for fertilization (Sprunck et al, 2012;Cyprys et al, 2019). As mentioned previously, the cysteine residues in CRPs are the potential targets of ROS signaling; thus, the implication of ROS in pollen tube guidance and fertilization is expected by mediating the redox modification of the CRPs.…”

Section: Are Ros Involved In Pollen Tube Guidance Growth and Fertlizamentioning

confidence: 93%

ROS in the Male–Female Interactions During Pollination: Function and Regulation

Zhang

Gao

2020

Front. Plant Sci.

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The male-female interactions in pollination mediate pollen hydration and germination, pollen tube growth and fertilization. Reactive oxygen species (ROS) derived from both male and female tissues play regulatory roles for the communication between the pollen/ pollen tube and female tissues at various stages, such as pollen hydration and germination on the stigma, pollen tube growth in the pistil and pollen tube reception in the female gametophyte. In this minireview, we primarily summarize the recent progress on the roles of ROS signaling in male-female interactions during pollination and discuss several ROSregulated downstream signaling pathways for these interactions. Furthermore, several ROS-involved downstream pathways are outlined, such as Ca 2+ signaling, cell wall cytomechanics, the redox modification of CRP, and cell PCD. At the end, we address the roles of ROS in pollen tube guidance and fertilization as future questions that merit study.

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Section: Are Ros Involved In Pollen Tube Guidance Growth and Fertlizamentioning

confidence: 93%

ROS in the Male–Female Interactions During Pollination: Function and Regulation

Zhang

Gao

2020

Front. Plant Sci.

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“…For gamete fusion, male gametic membrane proteins GENERATIVE CELL SPECIFIC 1/HAPLESS 2 (GCS1/HAP2) and GAMETE EXPRESSED 2 (GEX2) located on the surface of sperm cells directly regulate gamete fusion and attachment, respectively (von Besser, Frank, Johnson, & Preuss, ; Mori, Igawa, Tamiya, Miyagishima, & Berger, ; Mori, Kuroiwa, Higashiyama, & Kuroiwa, ). DOMAIN OF UNKNOWN FUNCTION 679 membrane proteins (DMP8 and DMP9) have also recently been identified as additional fertilization regulators involved in gamete interaction (Cyprys, Lindemeier, & Sprunck, ; Takahashi et al, ). Arabidopsis GCS1/HAP2 is structurally similar to viral fusion proteins and has an extracellular fusion loop which is hydrophobic and flanked by charged residues.…”

Section: Double Fertilization and Polyspermy Block In Angiospermsmentioning

confidence: 99%

“…(DMP8 and DMP9) have also recently been identified as additional fertilization regulators involved in gamete interaction (Cyprys, Lindemeier, & Sprunck, 2019;Takahashi et al, 2018).…”

Section: Domain Of Unknown Function 679 Membrane Proteinsmentioning

confidence: 99%

Polyspermy in angiosperms: Its contribution to polyploid formation and speciation

Toda

Okamoto

2019

Molecular Reproduction Devel

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Polyploidization has played a major role in the long‐term diversification and evolutionary success of angiosperms. Triploid formation among diploid plants, which is generally considered to be achieved by fertilization of an unreduced gamete with a reduced one, has been accepted as a means of polyploid production. In addition, it has been supposed that polyspermy also contributes to the triploid formation in maize, wheat, and some orchids; however, such a mechanism has been considered uncommon because reproducing the polyspermic situation and unambiguously investigating developmental profiles of polyspermic zygotes are difficult. To overcome these problems, rice polyspermic zygotes have been successfully produced by electrofusion of an egg cell with two sperm cells, and their developmental profiles have been monitored. The triploid zygotes progress through karyogamy and divide into two‐celled embryos via a typical bipolar mitotic division; the two‐celled embryos further develop into triploid plants, indicating that polyspermic plant zygotes, unlike those of animals, can develop normally. Furthermore, progenies consisting of triparental genetic materials have been successfully obtained in Arabidopsis through the pollination of two different kinds of male parents with a female parent. These different pieces of evidence for development and emergence of polyspermic zygotes in vitro and in planta suggest that polyspermy is a key event in polyploidization and species diversification.

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“…Within a short time after adhesion, one sperm cell fuses with the egg to generate the embryo, while the other sperm fuses with the central cell to generate the endosperm. Both of the new papers show that sperm with reduced DMP9 expression 8 or completely absent DMP8 and DMP9 expression 7 retained a higher capacity for fusion with the central cell than with the egg cell. These results likely are not a reflection of an inherent difference in the fusion capacity of the two female gametes, since the egg cell is preferentially fertilized in other fertilization-related mutants 13,14 .…”

mentioning

confidence: 95%

“…In contrast, HAP2 is just one of many proteins on the dynamic and multi-functional plasma membrane of gametes of eukaryotic organisms. In this issue of Nature Plants , Cyprys et al 7 report that HAP2-based fusion in Arabidopsis does indeed differ from viral class II fusion mechanisms, and that the fusion reaction works better with a little help from two conserved, short, multi-pass integral membrane proteins DMP8 and DMP9. Fertilization is reduced over 2-fold in Arabidopsis mutants lacking sperm-specific DMP8 and DMP9.…”

mentioning

confidence: 99%