Gametic embryogenesis through isolated microspore culture in Corylus avellana L.

Karasawa, Marines Marli Gniech; Chiancone, Benedetta; Gianguzzi, Valeria; Abdelgalel, A; Botta, Roberto; Sartor, Chiara; Germanà, María Antonietta

doi:10.1007/s11240-015-0921-1

Cited by 19 publications

(5 citation statements)

References 46 publications

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“…However, efficient and reliable techniques for hazelnut propagation by cuttings or micropropagation are still lacking, since the species is recalcitrant to in vivo -induced rhizogenesis (Silvestri et al, 2016). Moreover, micropropagation represents a useful technique not only for reproductive purpose in hazelnut but also for its genetic improvement (Bacchetta et al, 2008; Karasawa et al, 2016, Silvestri et al, 2016; Silvestri et al, 2018). Many attempts have been made for setting up suitable micropropagation protocols adapted for a wide range of hazelnut varieties, through the optimization of factors such as media components, hormones, explant types (Hand and Reed, 2014; Hand et al, 2014; Hand et al, 2016; Akin et al, 2017a; Akin et al, 2017b; Gentile et al, 2017; Akin et al, 2018; Pincelli-Souza et al, 2018; Silvestri et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Influence of Continuous Spectrum Light on Morphological Traits and Leaf Anatomy of Hazelnut Plantlets

et al. 2019

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Light spectra influence growth, development, and quality of plants and seedlings, that is one of the main aspects engaging the interests of private and public researchers and nursery industries. Propagation of hazelnut (Corylus avellana L.), which in the past has been held in low consideration because of the widespread use of rooted suckers directly collected in the field, today is taking on increasing interest due to the strong expansion of hazelnut cultivation. In order to improve the quality of plants and seedlings in greenhouse acclimatization, the effects of light emitting diodes (LED) lights during the ex vitro growth of two hazelnut cultivars (Tonda di Giffoni and Tonda Gentile Romana) were investigated. Plantlets were maintained in a growth chamber and exposed to three different continuous spectrum LED systems as a primary source of illumination and to fluorescent lamps used as control. LEDs differed in the percentage of some wavelength ranges in the spectrum, being AP673L rich in green and red wavelengths, NS1 in blue and green light, G2 in red and far red wavelengths. After a 4-week experimental period, morphometric, biochemical, and histological analyses were carried out. Shoot and leaf growths were influenced by LEDs more than by fluorescent lamps in both cultivars. G2 positively affected biomass increment more than the other LEDs, by inducing not only cell elongation (increase in shoot length, new internodes length, leaf area) but also cell proliferation (increase in new node number). G2 exposure had negative effects on total chlorophyll content but positively affected synthesis of flavonoids in both varieties; therefore, plants grown under this LED showed the lowest nitrogen balance index. Leaf morpho-anatomical analyzed traits (thickness, palisade cell height, number of chloroplasts, number of palisade cells), were influenced especially by G2 and, to a less extent, by NS1 light. Significant differences in some parameters were observed between the two cultivars in response to a same light source. The results obtained underline the importance of light modulation for hazelnut, providing useful information for ex vitro growth of hazelnut plantlets.

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Section: Introductionmentioning

confidence: 99%

Influence of Continuous Spectrum Light on Morphological Traits and Leaf Anatomy of Hazelnut Plantlets

et al. 2019

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“…As a closing remark, IFC turned out to be strikingly useful for the label-free characterization of hazelnut pollen in a high throughput, allowing the fast identification of hazelnut cultivars with high male sterility. Moreover, its versatility holds great promise for numerous research applications in hazelnut pollen biology and pollination ecology and has the potential to simplify haploid plant production for hazelnut breeding purposes ( Gniech Karasawa et al, 2016 ), as demonstrated by recent studies on wheat ( Canonge et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry

et al. 2020

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Impedance flow cytometry (IFC) is a versatile lab-on-chip technology which enables fast and label-free analysis of pollen grains in various plant species, promising new research possibilities in agriculture and plant breeding. Hazelnut is a monoecious, anemophilous species, exhibiting sporophytic self-incompatibility. Its pollen is dispersed by wind in midwinter when temperatures are still low and relative humidity is usually high. Previous research found that hazelnut can be characterized by high degrees of pollen sterility following a reciprocal chromosome translocation occurring in some cultivated genotypes. In this study, IFC was used for the first time to characterize hazelnut pollen biology. IFC was validated via dye exclusion in microscopy and employed to (i) follow pollen hydration over time to define the best pre-hydration treatment for pollen viability evaluation; (ii) test hazelnut pollen viability and sterility on 33 cultivars grown in a collection field located in central Italy, and two wild hazelnuts. The accessions were also characterized by their amount and distribution of catkins in the tree canopy. Pollen sterility rate greatly varied among hazelnut accessions, with one main group of highly sterile cultivars and a second group, comprising wild genotypes and the remaining cultivars, producing good quality pollen. The results support the hypothesis of recurring reciprocal translocation events in Corylus avellana cultivars, leading to the observed gametic semi-sterility. The measured hazelnut pollen viability was also strongly influenced by pollen hydration (Radj2 = 0.83, P ≤ 0.0001) and reached its maximum at around 6 h of pre-hydration in humid chambers. Viable and dead pollen were best discriminated at around the same time of pollen pre-hydration, suggesting that high humidity levels are required for hazelnut pollen to maintain its functionality. Altogether, our results detail the value of impedance flow cytometry for high throughput phenotyping of hazelnut pollen. Further research is required to clarify the causes of pollen sterility in hazelnut, to confirm the role of reciprocal chromosome translocations and to investigate its effects on plant productivity.

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“…Exhaustive investigations on the androgenesis using anther culture have been carried out on the type and concentration of plant growth regulators (Perera et al, 2009;Smykalova´ et al, 2009). Regarding culture medium, Chu N6 medium has been the most often used and effective medium for anther culture (Aboshama, 2011) and has induced gametic embryogenesis in various woody plant species like citrus, apricot, hazelnut, and loquat (Germanà and Chiancone, 2003;Karasawa et al, 2016;Cardoso et al, 2016). In the current study, Chu's N6 salts and Nitsch vitamins, with or without additional supplements, PGRs, and PVP, were used for callogenesis and subsequent embryogenesis.…”

Section: Effect Of Cold Treatment and Genotype On Pollen Viabilitymentioning

confidence: 99%

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

Bokhari

2023

PAKJAS

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Androgenesis is reported in a few tree crops such as citrus and papaya; however, there is little information available about guava. Different modified Chu (N6) media and cold pretreatments of floral buds were tested to induce embryogenic calli in the anthers of two white flesh guava cultivars, ‘Round’ and ‘Pyriform’. Four modified Chu (N6) media formulations, i.e., M1 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP, 0.5 mgL-1 zeatin and 5% coconut water), M2 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP), M10 (0.5 mgL-1 2,4-D), and M11 (0.5 mgL-1 2,4-D and 50 mgL-1 PVP) enhanced anther swelling and callus formation. Genotypic variability was found in pollen viability, phenolic exudation, and anther tissue browning after cold pretreatment for 0-72 hrs. intervals. Anthers of ‘Round’ showed more pollen viability across cold pretreatment intervals with less phenolic exudations and tissue browning than ‘Pyriform’. On both M10 and M11 media, anthers with uni-nucleate microspores developed embryogenic calli; however, anthers of ‘Pyriform’ resulted in more calli induction (69.58%) on M10 media, whereas anthers of ‘Round’ developed more calli (66.60%) on M11 media. The proliferating calli in white flesh ‘Round’ and ‘Pyriform’ cultivars and pink flesh ‘Round’ cultivar formed more embryogenic masses upon transfer to growth hormone-free Chu (N6) media under light conditions; however, embryos showed delayed maturity with limited germination. More genotypes shall be explored to enhance embryo germination, and embryogenic media shall be further fine-tuned. This study provides the foundation for the generation of homozygous lines in guava using androgenesis

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Gametic embryogenesis through isolated microspore culture in Corylus avellana L.

Cited by 19 publications

References 46 publications

Influence of Continuous Spectrum Light on Morphological Traits and Leaf Anatomy of Hazelnut Plantlets

Influence of Continuous Spectrum Light on Morphological Traits and Leaf Anatomy of Hazelnut Plantlets

Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

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