Riccardia pinguis (L.) S. F. Gray is found rather sparingly along the edge of the marsh next the wooded upland south of Lake Wingra (Madison, Wisconsin). The thalli grow on fallen willow canes, on the bases of standing willows, on the black mud, and occasionally on fallen leaves. In this locality they are well shaded during the summer when the willows and oaks are in full foliage, but are partly exposed during the fall and early spring.Plants of this species are somewhat more abundant on the railroad right-of-way thr.ough the swamp prairie bordering Lake Waubesa and extending westward for about a mile. At this station the thalli grow on the mud and cinders and more rarely on the roots and stubble of grasses. The grasses stand one half to one and one half meters high and form a fairly thick shade over the thalli during the summer months, but are burned off annually in the fall or spring. Numerous searches in the swamp on either side of the railroad have revealed no plants beyond the limits of the annual burning of the grass.In June, 1921, before the latter station had been discovered, the low prairie in the vicinity of Roby, Indiana, about twenty miles from Chicago, was visited in the hope of obtaining material for. cytological study. Plants were found in this region growing chiefly on decaying leaves about the bases of very small willows scattered through the more swampy portions of the prairie. This material was not in thriving condition because of insufficient moisture, and only a small collection was made.At all these stations the plants are protected from the summer sun but are favored with nearly full sunlight during the fall and early spring-the seasons during which vegetative growth appears to be most rapid. It was further observed that plants exposed, by tramping of the grass, to the August sun quickly succumbed.
FIELD AND CULTURE OBSERVATIONSThe first collections of plants for this study were made in the spring of 1920 from the region of Lake Wingra and consisted of only a few dozen thalli. These plants were used to start greenhouse cultures which grew very well during the summer and early fall of 1920, but later became contaminated with blue-green algae and had to be discarded early in the following winter. These cultures were grown on a thin layer of leaf mold over a substratum of sand in shallow wooden boxes standing in a tray of nutrient 148