Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Fisch, Paul; Malkovsky, Miroslav; Braakman, E.; Sturm, Els; Bolhuis, R. L. H.; Prieve, Audrey F.; Sosman, Jeffrey A.; Lam, Valery; Sondel, Paul M.

doi:10.1084/jem.171.5.1567

Cited by 160 publications

(83 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data are consistent with the results of the other researchers [21]. Like NK cells, Υ δ T cells in human peripheral blood mediate the non-MHC-restricted cytolytic effect against many tumor cells.…”

Section: Discussionsupporting

confidence: 83%

Selective proliferation of human γδ T cells in vitro

et al. 1996

View full text Add to dashboard Cite

The effect of monoethylphosphate (MEP, commercial available or synthesized) together with IL-2 on the selective proliferation of human Υ δ T cells in vitro from peripheral blood mononuclear cells (PBMC) of healthy donors and of cancer patients was investigated. The Υ δ T cells were stimulated by MEP to proliferate in a dose-dependent manner. The effect of synthesized MEP was 10 times greater than that of commercial MEP. When the PBMCs of healthy donors were cultured for 25 d in the medium containing different concentrations of MEP, the total cell number increased about 1000-3000 fold; and the ratio of Υ δ T cells reached to 70-80%. The selective expansion of Υ δ T cells depended on the synergic action of MEP and IL-2. The bulk cultured Υ δ T cells exhibited obvious cytotoxic activities against allogenic tumor cell lines (SQ-5, K562 and Daudi) and autologous tumor cells. The culture system described here not only offers a simple method for obtaining a large number of Υ δ T cells which may become a new effector in the adoptive immunotherapy, but also provides a useful model for the further studies of the structure and function of Υ δ T cells in vitro .

show abstract

Section: Discussionsupporting

confidence: 83%

Selective proliferation of human γδ T cells in vitro

et al. 1996

View full text Add to dashboard Cite

show abstract

“…The membrane localisation of HSP was observed directly by immunofluorescence on activated mononuclear phagocytes (Wurttenberg et al, 1991) and in the course of viral infections (La Thangue & Latchman, 1988), and indirectly by the fact that TT/6 lymphocytes recognises HSP on Daudi cells (Fish et al, 1990). In this context, HSP recognition might represent a mechanism for the role of HSP in autoimmunity and microbial infections (Kaufmann, 1990).…”

Section: Discussionmentioning

confidence: 99%

Recognition and killing of tumour cells expressing heat shock protein 65 kD with immunotoxins containing saporin

Poccia

Piselli²,

Cesare³

et al. 1992

Br J Cancer

View full text Add to dashboard Cite

SummaryThe expression of heat shock proteins (HSP) of the 65kD family (groEL) has been observed by flow cytometry using murine monoclonal antibody (MoAb) anti-HSP 65kD (ML30) on the surface of B (Daudi) or T (H9) lymphoma cells, on a moncyte cell line (U937) and also on a primary culture of a human pancreatic carcinoma (HPC). Moreover Heat Shock Proteins (HSP) represent a family of highly conserved molecules that under stress conditions play an important physiological role in folding and unfolding of proteins (Lindquist & Craig, 1988). HSP expression is strictly related to cell cycle and oncogene activation (Pechan, 1991), and it is important to note that HSP are often tumour associated antigens (Ulrich & Robinson, 1986;Srivistava & Maki, 1990). In fact, myc-overexpressing cells show viral myc-proteins nuclearly colocalised with nuclear HSP70 (Koshinen et al., 1991). The myc-oncogene is functionally similar to the adenovirus Ela and is able to collaborate with activated ras-oncogene to transform primary fibroblasts (Ralston, 1991). It has been reported also that the adenoviral Ela products induce HSP70 synthesis by acting as transcriptional activator. However, it is not yet clear whether increased levels of HSP may facilitate tumoural and viral proliferation. In Hela cells, HSP70 interacts with other cellular proteins in a cell cycle-dependent manner; synthesis of HSP has been shown to increase during mitosis (Pechan, 1991). Other HSP such as HSP90 and HSP70 have been found to reach abnormally high levels in transformed cells (Bensaude & Morange, 1983). Proteins belonging to the HSP70 family were shown to interact with nuclear oncogenes such as p53, and the stability of this interaction might influence transformation (Finlay et al., 1988 SCID miceThe SCID mutation (Bosma et al., 1983) occurred in the C.B-171cr (C.B-17) inbred strain, an immunoglobulin heavy chain (Igh) congenic partner strain of BALB/cAnIcr (BALB/ c). These mice have a severe combined immune deficiency as a result of their inability to rearrange correctly their immunoglobulin and T-cell receptor genes, and for this reason are permissive for the growing of transformed human Correspondence: V. Colizzi,

show abstract

“…Esslin and Formby 7 showed that in vitro activated peripheral blood ␥␦ + T cells possess MHC non-restricted cytolytic activity towards selected human tumor cell lines when compared to similarly activated ␣␤ + T cells. Others have shown that heat shock proteins, [17][18][19] TCT.1 20 and the nonclassical human MHC antigens MICA and MICB 16 also function as ligands for ␥␦ + T cells. Cytotoxic ␥␦ + T cells have been generated against EBV-transformed B cells, 21 Burkitt lymphoma cells, 21 Daudi lymphoma cells, 22 and against autologous maturing hematopoietic cells from a patient with aplastic anemia.…”

Section: Discussionmentioning

confidence: 99%

Human γδ+ T lymphocytes have in vitro graft vs leukemia activity in the absence of an allogeneic response

Lamb

Musk

et al. 2001

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:Refractory acute lymphoblastic leukemia (ALL) is often incurable, and relapse rates following allogeneic bone marrow transplantation (BMT) remain high. We have reported that patients who develop increased numbers of ␥␦ + T cells soon after BMT are significantly less likely to relapse. We now show in seven donor/recipient pairs that donor-derived V␦1 + CD4 − CD8 − ␥␦ + T cells are activated and proliferate in response to recipient primary ALL blasts. In addition, these cells have been shown to bind and lyse the recipient ALL blasts. Separately, ␥␦ + T cells proliferate poorly or not at all in mixed lymphocyte culture against HLA-mismatched unrelated stimulator cells. These observations suggest that allogeneic ␥␦ + T cells could be an effective immunotherapeutic strategy against refractory disease without the risk of graft-versus-host disease. Bone Marrow Transplantation (2001) 27, 601-606.

show abstract

Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis.

Cited by 160 publications

References 35 publications

Selective proliferation of human γδ T cells in vitro

Selective proliferation of human γδ T cells in vitro

Recognition and killing of tumour cells expressing heat shock protein 65 kD with immunotoxins containing saporin

Human γδ+ T lymphocytes have in vitro graft vs leukemia activity in the absence of an allogeneic response

Contact Info

Product

Resources

About