Gamma-H2AX Foci Decay Ratio as a Stronger Predictive Factor of Late Radiation Toxicity Than Dose-Volume Parameters in a Prospective Cohort of Prostate Cancer Patients

Nuijens, Anna C.; Oei, Arlene L.; Oorschot, Bregje van; Visser, Jelle; Os, Rob M. van; Moerland, Perry D.; Franken, Nicolaas A.P.; Rasch, Coen R. N.; Stalpers, Lukas J.A.

doi:10.1016/j.ijrobp.2021.08.020

Cited by 7 publications

(8 citation statements)

References 35 publications

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“…Data from 179 patients were used for a recent analysis concerning the relevance of genetic predisposition, especially compared with dose-volume factors, to the risk of late radiation toxicity [ 10 ]. All patients were treated with intensity-modulated radiation therapy (IMRT).…”

Section: Methodsmentioning

confidence: 99%

“…Lastly, univariable logistic regression was performed to investigate whether the γ-H2AX FDR was associated with the long-term (period) prevalence of toxicity (i.e., according to the physician and/or to the patients). The FDR was dichotomized as < or ≥3.41, this threshold was determined in a previous retrospective study and validated in a prospective study and a recent re-evaluation of this latter study [ 10 , 35 , 40 ]. Groups were formed according to the presence (or lack) of moderate or worse toxicity in the previous week (PROM) or month (CTCAE).…”

Section: Methodsmentioning

confidence: 99%

“…Consequently, a large number of patients are at risk of developing late radiation toxicity. In fact, around 30% of patients develop moderate or severe late radiation toxicity, although reported toxicity rates vary widely [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Symptoms can persist for a long time, if not permanently, and may significantly affect quality of life (QoL) [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we found that a less efficient repair of DNA double-strand breaks (DSBs) in ex vivo irradiated lymphocytes, as quantified by the γ-H2AX foci decay ratio (FDR), is an independent risk factor for the development of CTCAE grade ≥ 2 late toxicity in prostate cancer patients treated with EBRT [ 10 ]. Determining a patient’s γ-H2AX FDR before treatment may be an interesting additional tool, apart from looking into dose-volume parameters, to predict the individual risk for radiation toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…For the present study, we reached out to the prostate cancer patients included in our previous study to obtain long-term toxicity data [ 10 ]. The first aim of the present study was to report and compare the results of physician-assessed toxicity with patient-reported symptoms.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison between Patient- and Physician-Reported Late Radiation Toxicity in Long-Term Prostate Cancer Survivors

Nuijens

Oei

Bouhuijs

et al. 2022

Cancers

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Patient-reported outcome measures (PROMs) are advocated for the monitoring of toxicity after radiotherapy. However, studies comparing physician- and patient-reported toxicity show low concordance. In this study, we compared physician- and patient-reported toxicity in long-term prostate cancer survivors after radiotherapy, and we determined the correlation with a presumable risk factor for late toxicity: γ-H2AX foci decay ratio (FDR). Patients formerly included in a prospective study were invited to participate in this new study, comprising one questionnaire and one call with a trial physician assistant. Concordance was calculated for seven symptoms. Gamma-H2AX FDRs were determined in ex vivo irradiated lymphocytes in a previous analysis. Associations between FDR and long-term prevalence of toxicity were assessed using univariable logistic regression analyses. The 101 participants had a median follow-up period of 9 years. Outcomes were discordant in 71% of symptomatic patients; in 21%, the physician-assessed toxicity (using CTCAE) was higher, and, in 50%, the patients reported higher toxicity. We did not find a correlation between presence of toxicity at long-term follow-up and FDR. In conclusion, patients assigned greater severity to symptoms than the trial physician assistant did. Consideration of both perspectives may be warranted to provide the best care.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Comparison between Patient- and Physician-Reported Late Radiation Toxicity in Long-Term Prostate Cancer Survivors

Nuijens

Oei

Bouhuijs

et al. 2022

Cancers

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Considerations for the Use of the DNA Damage Marker γ‐H2AX in Disease Modeling, Detection, Diagnosis, and Prognosis

Hosking,

Pederick,

Neilsen

et al. 2024

Aging and Cancer

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BackgroundDNA double‐strand breaks are known to be the most lethal kind of DNA damage as they cause genomic instability. Visualization and quantification of these breaks are possible via staining of the phosphorylated histone H2A variant H2AX at serine 139 with the anti‐phospho‐histone H2AX (γ‐H2AX) antibody.MethodsThe literature was searched with the following keywords: DNA damage, double‐strand break, PBMC, DNA repair, H2AX, γ‐H2AX.DiscussionThis review discusses various methods for the quantification of γ‐H2AX and the use of γ‐H2AX in cell culture, tissue biopsies, and peripheral blood mononuclear cells. The current research into basal DNA damage as quantified by γ‐H2AX is discussed in relation to cancer.ConclusionsThis review suggests that γ‐H2AX has the potential for use in the prediction of cancer risk when applied to healthy tissue and peripheral blood mononuclear cells.

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Predicting Radiation-Induced Lung Injury in Patients With Lung Cancer: Challenges and Opportunities

Kuipers,

van Doorn-Wink,

Hiemstra

et al. 2024

International Journal of Radiation Oncology*Biology*Physics

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Gamma-H2AX Foci Decay Ratio as a Stronger Predictive Factor of Late Radiation Toxicity Than Dose-Volume Parameters in a Prospective Cohort of Prostate Cancer Patients

Cited by 7 publications

References 35 publications

A Comparison between Patient- and Physician-Reported Late Radiation Toxicity in Long-Term Prostate Cancer Survivors

A Comparison between Patient- and Physician-Reported Late Radiation Toxicity in Long-Term Prostate Cancer Survivors

Considerations for the Use of the DNA Damage Marker γ‐H2AX in Disease Modeling, Detection, Diagnosis, and Prognosis

Predicting Radiation-Induced Lung Injury in Patients With Lung Cancer: Challenges and Opportunities

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