Gamma-irradiated human amniotic membrane decellularised with sodium dodecyl sulfate is a more efficient substrate for the ex vivo expansion of limbal stem cells

Figueiredo, Gustavo S.; Bojić, Sanja; Rooney, Paul; Wilshaw, Stacy‐Paul; Connon, Che J.; Gouveia, Ricardo M.; Paterson, Carl; Lepert, Guillaume; Mudhar, Hardeep Singh; Figueiredo, Francisco C; Lako, Majlinda

doi:10.1016/j.actbio.2017.07.041

Cited by 28 publications

(16 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most important aspects of any tissue decellularization protocol are to eliminate the risk of immune rejection and disease transmission, while at the same time retaining the underlying ECM structure. In this regard, Figueiredo et al (2017) studied the potential use of decellularized human amniotic membrane for the ex vivo expansion of LSCs. They also evaluated, using gamma irradiation as a final sterilization step, the potential minimization of risk regarding disease transmission.…”

Section: Corneal Wound Healingmentioning

confidence: 99%

Corneal Repair and Regeneration: Current Concepts and Future Directions

Mobaraki

Abbasi

Vandchali

et al. 2019

Front. Bioeng. Biotechnol.

125

109

View full text Add to dashboard Cite

The cornea is a unique tissue and the most powerful focusing element of the eye, known as a window to the eye. Infectious or non-infectious diseases might cause severe visual impairments that need medical intervention to restore patients' vision. The most prominent characteristics of the cornea are its mechanical strength and transparency, which are indeed the most important criteria considerations when reconstructing the injured cornea. Corneal strength comes from about 200 collagen lamellae which criss-cross the cornea in different directions and comprise nearly 90% of the thickness of the cornea. Regarding corneal transparency, the specific characteristics of the cornea include its immune and angiogenic privilege besides its limbus zone. On the other hand, angiogenic privilege involves several active cascades in which anti-angiogenic factors are produced to compensate for the enhanced production of proangiogenic factors after wound healing. Limbus of the cornea forms a border between the corneal and conjunctival epithelium, and its limbal stem cells (LSCs) are essential in maintenance and repair of the adult cornea through its support of corneal epithelial tissue repair and regeneration. As a result, the main factors which threaten the corneal clarity are inflammatory reactions, neovascularization, and limbal deficiency. In fact, the influx of inflammatory cells causes scar formation and destruction of the limbus zone. Current studies about wound healing treatment focus on corneal characteristics such as the immune response, angiogenesis, and cell signaling. In this review, studied topics related to wound healing and new approaches in cornea regeneration, which are mostly related to the criteria mentioned above, will be discussed.

show abstract

Section: Corneal Wound Healingmentioning

confidence: 99%

Corneal Repair and Regeneration: Current Concepts and Future Directions

Mobaraki

Abbasi

Vandchali

et al. 2019

Front. Bioeng. Biotechnol.

125

109

View full text Add to dashboard Cite

show abstract

“…Conversely, BMT can be used to better design the appropriate mechanical properties in natural or manufactured carriers for LESC transplantation or the engineering of tissue constructs. Indeed, the prevalent use of substrates with limbus-like compliance (e.g., fibrin gels, hAM) 74,75 for the ex vivo expansion of LESCs is, however accidental, probably incidental to the success of the subsequent transplantation. Ultimately, the use of BMT to control the mechanical properties of tissues in injured corneas may represent, alone or in combination, the most sophisticated, single-use, non-intrusive strategy for achieving corneal repair based on the long-lasting restoration of normal cellular healing responses (Fig.…”

Section: Biomechanical Modulation Therapymentioning

confidence: 99%

Biomechanical Modulation Therapy—A Stem Cell Therapy Without Stem Cells for the Treatment of Severe Ocular Burns

Gouveia

Connon

2020

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

Ocular injuries caused by chemical and thermal burns are often unmanageable and frequently result in disfigurement, corneal haze/opacification, and vision loss. Currently, a considerable number of surgical and pharmacological approaches are available to treat such injuries at either an acute or a chronic stage. However, these existing interventions are mainly directed at (and limited to) suppressing corneal inflammation and neovascularization while promoting re-epithelialization. Reconstruction of the ocular surface represents a suitable but last-option recourse in cases where epithelial healing is severely impaired, such as due to limbal stem cell deficiency. In this concise review, we discuss how biomechanical modulation therapy (BMT) may represent a more effective approach to promoting the regeneration of ocular tissues affected by burn injuries via restoration of the limbal stem cell niche. Specifically, the scientific basis supporting this new therapeutic modality is described, along with our growing understanding of the role that tissue biomechanics plays in stem cell fate and function. The potential impact of BMT as a future treatment option for the management of injuries affecting tissue compliance is also further discussed.

show abstract

“…Brillouin spectroscopy is a non-destructive and label-free approach to determining viscoelasticity of a wide range of materials. Brillouin spectroscopy has been successfully applied in recent years in a number of biological applications [32][33][34][35][36][37][38][39][40]. Brillouin spectroscopy nondestructively measures elasticity with high spatial resolution, making it a feasible method to differentiate between healthy tissue and tumors.…”

Section: Introductionmentioning

confidence: 99%

Differentiating melanoma and healthy tissues based on elasticity-specific Brillouin microspectroscopy

Troyanova‐Wood

Meng

Yakovlev

2019

Biomed. Opt. Express

View full text Add to dashboard Cite

The main objective of the present study is to evaluate the use of Brillouin microspectroscopy for differentiation of melanoma and normal tissues based on elasticity measurements. Previous studies of malignant melanoma show that the lesion is stiffer than the surrounding healthy tissue. We hypothesize that elasticity-specific Brillouin spectroscopy can be used to distinguish between healthy and cancerous regions of an excised melanoma from a Sinclair miniature swine. Brillouin measurements of non-regressing and regressing melanomas and the surrounding healthy tissues were performed. Based on the Brillouin measurements, the melanomas and healthy tissues can be successfully differentiated. The stiffness of both tumors is found to be significantly greater than the healthy tissues. Notably, we found that the elasticity of regressing melanoma is closer to that of the normal tissue. The results indicate that Brillouin spectroscopy can be utilized as a tool for elasticity-based differentiation between malignant melanoma and surrounding healthy tissue, with potential use for melanoma boundary identification, monitoring tumor progression, or response to treatment.

show abstract

Gamma-irradiated human amniotic membrane decellularised with sodium dodecyl sulfate is a more efficient substrate for the ex vivo expansion of limbal stem cells

Abstract: Graphical abstract

Cited by 28 publications

References 46 publications

Corneal Repair and Regeneration: Current Concepts and Future Directions

Corneal Repair and Regeneration: Current Concepts and Future Directions

Biomechanical Modulation Therapy—A Stem Cell Therapy Without Stem Cells for the Treatment of Severe Ocular Burns

Differentiating melanoma and healthy tissues based on elasticity-specific Brillouin microspectroscopy

Contact Info

Product

Resources

About