Gamma irradiated protease from Echis pyramidum venom: A promising immunogen to improve viper bites treatment

El-Yamany, Mohammed F.; Samy, Esraa M.; Salama, Walaa H.; Shaaban, Esmat A.; El-Latif, Hekma A. Abd

doi:10.1016/j.toxicon.2020.10.013

Cited by 3 publications

(2 citation statements)

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“…To investigate the properties of the purified Epy-Hase enzyme, the turbidmetric assay described by Pukrittayakamee et al [10] was performed with slight modifications. The optimum pH was examined by using several assay buffers of different pHs at concentrations of 0.2 M containing 0.15 M NaCl as follows: sodium citrate (3-4), sodium acetate (4-6), sodium phosphate (6-7), and Tris-HCl (7)(8)(9). The reaction mixture was incubated at 37 °C for 15 min and the enzymatic activity was read at 400 nm.…”

Section: Biochemical Characterization Of Purified Hyaluronidase Enzymementioning

confidence: 99%

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Purification and Biochemical Characterization of Hyaluronidase from Egyptian Echis pyramidum pyramidum Venom

Salama

2024

Egypt. J. Chem.

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A novel hyaluronidase was purified to apparent homogeneity from Egyptian Echis pyramidum pyramidum venom. The enzyme was purified 7.6-fold by Sephacryl S-200 size exclusion followed by CM-Sepharose cation-exchange chromatography with a specific activity of 610 U/mg over the activity of whole venom (80 U/mg). The molecular mass of the purified enzyme was approximately 48 kDa in its native and reduced forms. The maximum activity was estimated at pH 5.5 and 40 o C. The purified hyaluronidase has an absolute activity towards hyaluronic acid with Km and Vmax values of 20µg/ml and 40µg/min, respectively. The Na + ion enhanced the hyaluronidase activity, whereas Fe +2 , Ni +2 , Cu +2 , Co +2 , Zn +2 , Mn +2 , o-phenanthroline (5 mM) and heparin (10 units) inhibited the enzyme activity by 100%. Antisera against the venoms of Echis pyramidum, Echis coloratus, Cerastes cerastes, Cerastes vipera and Naja haje greatly neutralized E. pyramidum hyaluronidase to a varied extent. In contrast, antisera against Naja nigricollis negatively neutralized the hyaluronidase activity. The study concluded that the purified enzyme shared similar immunogenic properties with venoms of vipers rather than elapids. It also demonstrated the importance of characterization of enzymes from venoms of different species to explore the venomics and antivenomics of Egyptian snake species.

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Section: Biochemical Characterization Of Purified Hyaluronidase Enzymementioning

confidence: 99%

“…Echis pyramidum pyramidum (Egyptian saw-scaled viper) belongs to the Viperidae family, is a highly medically important viper, and inhabits northern Egypt, particularly El-Faiyum and Giza deserts. Annually, more than 60% of snakebite deaths in Africa are caused by Echis vipers [9].…”

Section: Introductionmentioning

confidence: 99%