Gamma irradiation induces a pro-apoptotic state in longer stored platelets, without progressing to an overt apoptosis by day 7 of storage

Hosseini, Ehteramolsadat; Nodeh, Fatemeh Kiani; Ghasemzadeh, Mehran

doi:10.1007/s10495-023-01841-5

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…Pathogen reduction technologies (PRTs) reduce microbial contamination to secure platelet transfusions by chemical or physical (ultraviolet light illumination) methods but may lead to platelet activation, 19 ROS generation, PS exposure and apoptotic feature. 20 , 21 Even gamma irradiation as the known safe method also showed to increase ROS generation and pro‐apoptotic function in platelets albeit it does not increase the storage dependent levels of an overt apoptosis. 21 Leukoreduction by filtration can effectively decrease cytokine concentrations.…”

Section: Preparation and Storage Of Pc In The Blood Bankmentioning

confidence: 99%

“… 20 , 21 Even gamma irradiation as the known safe method also showed to increase ROS generation and pro‐apoptotic function in platelets albeit it does not increase the storage dependent levels of an overt apoptosis. 21 Leukoreduction by filtration can effectively decrease cytokine concentrations. 22 PAS as storage media have protective effects on platelets.…”

Section: Preparation and Storage Of Pc In The Blood Bankmentioning

confidence: 99%

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The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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BackgroundPlatelet concentrate (PC) transfusions are crucial in prevention and treatment of bleeding in infection, surgery, leukemia, and thrombocytopenia patients. Although the technology for platelet preparation and storage has evolved over the decades, there are still challenges in the demand for platelets in blood banks because the platelet shelf life is limited to 5 days due to bacterial contamination and platelet storage lesions (PSLs) at 20–24°C under constant horizontal agitation. In addition, the relations between some adverse effects of platelet transfusions and PSLs have also been considered. Therefore, understanding the mechanisms of PSLs is conducive to obtaining high quality platelets and facilitating safe and effective platelet transfusions.ObjectiveThis review summarizes developments in mechanistic research of PSLs and their relationship with clinical practice, providing insights for future research.MethodsAuthors conducted a search on PubMed and Web of Science using the professional terms “PSL” and “platelet transfusion.” The obtained literature was then roughly categorized based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section.ResultsDifferent studies have explored PSLs from various perspectives, including changes in platelet morphology, surface molecules, biological response modifiers (BMRs), metabolism, and proteins and RNA, in an attempt to monitor PSLs and identify intervention targets that could alleviate PSLs. Moreover, novel platelet storage conditions, including platelet additive solutions (PAS) and reconsidered cold storage methods, are explored. There are two approaches to obtaining high‐quality platelets. One approach simulates the in vivo environment to maintain platelet activity, while the other keeps platelets at a low activity level in vitro under low temperatures.ConclusionUnderstanding PSLs helps us identify good intervention targets and assess the therapeutic effects of different PSLs stages for different patients.

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Section: Preparation and Storage Of Pc In The Blood Bankmentioning

confidence: 99%

Section: Preparation and Storage Of Pc In The Blood Bankmentioning

confidence: 99%

The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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“…Platelets enter the circulation after the separation of bone marrow megakaryocytes, as anucleate cells (2 μm in diameter) that lack genomic DNA [ 1 ]. Therefore, due to the absence of a nucleus and in the presence of little capacity for protein synthesis, which is only available by messenger RNA (mRNA) transferred from the megakaryocyte, the lifespan of platelets is limited to 7–10 days [ 2 , 3 ]; however, platelets contain mitochondria that have also been transferred from megakaryocyte origin, and therefore, like nucleated cells, they can still benefit from flexible aerobic and anaerobic metabolism in the presence of necrotic and apoptotic pathways that regulate their function and fate [ 4 ]. Thrombopoietin is a glycoprotein synthesized mostly in the liver but also in the kidneys, which regulates megakaryocytic proliferation and maturation, as well as platelet production [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Platelets and the Atherosclerotic Process: An Overview of New Markers of Platelet Activation and Reactivity, and Their Implications in Primary and Secondary Prevention

Nardin,

Verdoia,

Cao

et al. 2023

JCM

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The key role played by platelets in the atherosclerosis physiopathology, especially in the acute setting, is ascertained: they are the main actors during thrombus formation and, thus, one of the major investigated elements related to atherothrombotic process involving coronary arteries. Platelets have been studied from different points of view, according with the technology advances and the improvement in the hemostasis knowledge achieved in the last years. Morphology and reactivity constitute the first aspects investigated related to platelets with a significant body of evidence published linking a number of their values and markers to coronary artery disease and cardiovascular events. Recently, the impact of genetics on platelet activation has been explored with promising findings as additional instrument for patient risk stratification; however, this deserves further confirmations. Moreover, the interplay between immune system and platelets has been partially elucidated in the last years, providing intriguing elements that will be basic components for future research to better understand platelet regulation and improve cardiovascular outcome of patients.

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