Gamma-irradiation of cacao (Theobroma cacao L.) pollen: Effect on pollen grain viability, germination and mitosis and on fruit set

Falque, Matthieu; Kodia, Afef; Sounigo, Olivier; Eskes, Albertus; Charrier, André

doi:10.1007/bf00046045

Cited by 25 publications

(17 citation statements)

References 15 publications

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“…styriaca) demonstrated a gradual reduction in fruit set and mean number of embryos from the control (55.6% and 73.4) to 350 Gy (25.0% and 18.8), respectively. Fruit set reduction with increasing radiation doses was also reported in melon (Lotfi et al, 2003), European plum (Peixe et al, 2000), and cacao (Falque et al, 1992). Here in iris it seems that the fruit set and haploid production is, after all, hardly dependent on the type of applied radiation and the amount of radiation dose.…”

Section: Fruit Set and Haploid Productionsupporting

confidence: 58%

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Grouh¹,

Sousaraei²,

Akbari³

et al. 2015

Turk J Agric For

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This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males. Anthers collected from the pollen donor were irradiated by X-ray with doses of 0, 100, 200, 300, and 400 Gy in 2012. A second round of experiments was conducted in 2013 with 300 and 400 Gy. The ovaries from each pollinated flower were collected and cultured and the produced plantlets were then acclimatized. Chromosome counting and flow cytometry were used to determine the ploidy levels. The viability of irradiated pollens was also evaluated. Pollination with irradiated pollen at doses ranging from 100 to 400 Gy can significantly reduce fruit set, particularly at higher doses (300 and 400 Gy). The embryo production was reduced in I. pseudacorus as doses increased; the highest doses led to reduction in the number of regenerants with the formation of just 6 and 4 embryos at 300 and 400 Gy, respectively. Haploid plantlets were produced when the pollen grains were exposed to 300 and 400 Gy of X-ray. Irradiation at 100 and 200 Gy was found to be insufficient to cause pollen sterility. The best dose for haploid production in I. pseudacorus is hereby found to be 300 or 400 Gy of X-ray.

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Section: Fruit Set and Haploid Productionsupporting

confidence: 58%

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Grouh¹,

Sousaraei²,

Akbari³

et al. 2015

Turk J Agric For

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“…A number of studies have shown that after pollination with irradiated pollen, seeds with only endosperm are produced in apple (James et al 1985;Nicoll et al 1987;Zhang and Lespinasse 1991), cacao (Falque et al 1992), cucumber (Le Deunff and Sauton 1994) and kiwifruit (Musial and Przywara 1998). In vitro culture of unfertilized ovules induced autonomous endosperm in cotton (Jensen et al 1977), barley (Huang et al 1982), rice (Zhou and Yang 1981), Melandrium album, Helleborus niger and Lupinus luteus (Mól 1992;Mól et al 1995), and Brassica napus (Chmielowiec et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Wijowska¹,

Kuta²,

Przywara³

1999

Sexual Plant Reproduction

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Autonomous endosperm was found in unfertilized ovules of V. odorata L. cultured on MS medium supplemented with 2,4-D as a sole growth regulator or on media with 2,4-D and BAP or kinetin. Frequency of endosperm induction was approximately 9% in ovules analyzed. The induction rate depended mainly on genotype of the donor plant, and to lesser degrees, on floral stage, flower series and medium type. Multinuclear endosperms consisting of 10-37 nuclei were found in ovules after as few as 4 days of culture. In some ovules at this stage, the egg cell and two polar nuclei were present. The process of endosperm degeneration began after 3 weeks of culture. In some ovules, degenerating autonomous endosperm was observed up to the 7th week. Parthenogenetic development of egg cells or apogamy did not accompany autonomous endosperm, supporting the hypothesis of independent pathways for embryo and endosperm development.

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“…Anthers were treated with FDA solution (10 mg FDA dissolved in 1 ml acetone as stock, diluted with 7% sucrose to a concentration of 100 µg/ml for use) for 5 min, and then photographed under ultraviolet light (Leica DMR, Germany) [70]. Pollen grains emitting green fluorescence were regarded as viable pollen.…”

Section: Phenotypic Analysismentioning

confidence: 99%

Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice

2009

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The Arabidopsis SDS (SOLO DANCERS) and RCK (ROCK-N-ROLLERS) genes are important for male meiosis, but it is still unknown whether they represent conserved functions in plants. We have performed phylogenetic analyses of SDS and RCK and their respective homologs, and identified their putative orthologs in poplar and rice. Quantitative real-time RT-PCR analysis indicated that rice SDS and RCK are expressed preferentially in young flowers, and transgenic RNAi rice lines with reduced expression of these genes exhibited normal vegetative development, but showed significantly reduced fertility with partially sterile flowers and defective pollens. SDS deficiency also caused a decrease in pollen amounts. Further cytological examination of male meiocytes revealed that the SDS deficiency led to defects in homolog interaction and bivalent formation in meiotic prophase I, and RCK deficiency resulted in defective meiotic crossover formation. These results indicate that rice SDS and RCK genes have similar functions to their Arabidopsis orthologs. Because rice and Arabidopsis, respectively, are members of monocots and eudicots, two largest groups of flowering plants, our results suggest that the functions of SDS and RCK are likely conserved in flowering plants.

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Gamma-irradiation of cacao (Theobroma cacao L.) pollen: Effect on pollen grain viability, germination and mitosis and on fruit set

Cited by 25 publications

References 15 publications

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice

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