Gamma irradiation of human platelet lysate: validation of efficacy for pathogen reduction and assessment of impacts on hpl performance

Huang, Chao; Liang, Fucheng; Lin, Yi-Chang; Chen, Y.; Tseng, Rong-Jeng; Huang, Mei-Han

doi:10.1016/j.jcyt.2019.03.498

Cited by 2 publications

(1 citation statement)

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“…However, there are very few of these options available, namely for MSC culture. Therefore, presently, hPL seems to be the most promising and cost-effective alternative to FBS supplementation in cell culture medium for now, being more readily translatable to a clinical setting, especially considering that gamma irradiated hPL products allowing significant viral reduction have already been developed (Huang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Scalable Production of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Under Serum-/Xeno-Free Conditions in a Microcarrier-Based Bioreactor Culture System

Fuzeta

Bernardes

Oliveira

et al. 2020

Front. Cell Dev. Biol.

105

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MSC-Derived EV Production in Bioreactors concentration (i.e., EV concentration in the conditioned medium) (5.7-fold increase overall) and productivity (i.e., amount of EVs generated per cell) (3-fold increase overall). BM, AT and UCM MSC cultured in the VWBR system yielded an average of 2.8 ± 0.1 × 10 11 , 3.1 ± 1.3 × 10 11 , and 4.1 ± 1.7 × 10 11 EV particles (n = 3), respectively, in a 60 mL final volume. This bioreactor system also allowed to obtain a more robust MSC-EV production, regarding their purity, compared to static culture. Overall, we demonstrate that this scalable culture system can robustly manufacture EVs from MSC derived from different tissue sources, toward the development of novel therapeutic products.

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Section: Discussionmentioning

confidence: 99%