Gamma-Linolenic Acid (GLA) Protects against Ionizing Radiation-Induced Damage: An In Vitro and In Vivo Study

Rengachar, Poorani; Bhatt, Anant Narayan; Polavarapu, Sailaja; Veeramani, Senthil; Krishnan, Anand; Sadananda, Monika; Das, Undurti N.

doi:10.3390/biom12060797

Cited by 11 publications

(10 citation statements)

References 86 publications

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“…GLA, DGLA, and PGE1 have potent cytoprotective and genoprotective actions against radiation, benzo(a)pyrene, 4α-phorbol, and diphenylhydantoin both in vitro and in vivo. [7][8][9][10][11][12][13] Similar cytoprotective action has also been reported with LXA4, resolvins and protectins. [14][15][16][17][18] Hence, it is suggested that these bioactive lipids prevent doxorubicin-induced cardiotoxicity by enhancing the formation of LXA4.…”

Section: Efas and Their Metabolites Are Cytoprotective And Genoprotec...supporting

confidence: 58%

Pyridoxine, essential fatty acids, and protection against doxorubicin‐induced cardiotoxicity

Das

2024

J Biochem & Molecular Tox

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Sun et al. [1] showed that vitamin B6 (pyridoxine) protects against doxorubicin-(DOX) induced cardiotoxicity by its antioxidant and antiapoptotic actions principally by decreasing NHE1 (Na + /H + exchanger) expression and thus, improved DOX-induced cardiotoxicity. I would like to suggest an alternative explanation for the interesting results obtained.Data sharing is not applicable to this article as no new data were created or analyzed in this study.

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Section: Efas and Their Metabolites Are Cytoprotective And Genoprotec...supporting

confidence: 58%

Pyridoxine, essential fatty acids, and protection against doxorubicin‐induced cardiotoxicity

Das

2024

J Biochem & Molecular Tox

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“…According to previous reports, irradiation‐induced salivary gland damage is attributed to elevated levels of oxygen free radicals induced by irradiation. Increased oxygen free radicals could cause abnormal inflammatory responses 42 . TNF‐α, IL‐1β and IL‐6 are very important proinflammatory cytokines with a range of biological activities 43 (Figure 3H).…”

Section: Resultsmentioning

confidence: 99%

“…Increased oxygen free radicals could cause abnormal inflammatory responses. 42 TNF‐α, IL‐1β and IL‐6 are very important proinflammatory cytokines with a range of biological activities 43 (Figure 3H ). We detected the expression of TNF‐α, IL‐1β and IL‐6 via western blotting.…”

Section: Resultsmentioning

confidence: 99%

Tetrahedral framework nucleic acids alleviate irradiation‐induced salivary gland damage

Xie

Guo

et al. 2022

Cell Proliferation

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In this study, we investigated the role of tetrahedral framework nucleic acids (tFNAs) in irradiation-induced salivary gland damage in vitro and in vivo. Irradiation-damaged submandibular gland cells (SMGCs) were treated with different concentrations of tFNAs. Cell activity was measured by CCK-8 assay. Cell death was detected by Calcein-AM/PI double staining. Cell apoptosis was assessed by flow cytometry. The expression of apoptosis proteins and inflammatory cytokines were detected by western blot. Body weight, drinking volume, saliva flow rate and lag time was measured 8 weeks after irradiation. Micromorphological changes of submandibular gland were assessed by haematoxylin-eosin and masson staining. Cell proliferation, apoptosis and microvessel density of submandibular gland were evaluated by immunohistochemical staining. tFNAs could promote cell proliferation, inhibit cell apoptosis of irradiation-damaged SMGCs and reduce irradiation induced cell death. Mechanism studies revealed that tFNAs inhibited cell apoptosis through regulating the Bcl-2/Bax/Caspase-3 signalling pathway and inhibited the release of TNF-α, IL-1β and IL-6 to reduce cell damage caused by inflammation. Animal experiments showed that tFNAs could alleviate irradiation-induced weight loss, increased water intake, decreased saliva production and prolonged salivation lag time and could ameliorate salivary gland damage. tFNAs have a positive effect on alleviating irradiation-induced salivary gland damage and might be a promising agent for the treatment of this disease. | INTRODUCTIONRadiotherapy is a vital treatment for head and neck cancer. Approximately 80% of patients suffering from head and neck cancer have received radiotherapy. However, radiotherapy can cause many Xueping Xie and Wenjuan Ma contribute equally to this work.

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“…This pathway resulted in a decrease in the abundance of gamma-linolenic acid (GLA) in the liver, ileum, colon, and kidney of colitis mice. GLA is an omega-6, 18 carbon PUFA, that is consumed in the diet (62). GLA is rapidly enzymatically converted into dihomo- gamma-linolenic acid (DGLA) and due to continuous conversion, it is naturally found at low levels with a corresponding increase in DGLA (63).…”

Section: Discussionmentioning

confidence: 99%

Spatial mapping of dextran sodium sulphate-induced intestinal inflammation and its systemic effects

Adams,

Rasid,

Hulme

et al. 2024

Preprint

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Inflammatory bowel disease (IBD) is a multifactorial disease and patients frequently experience extraintestinal manifestations affecting multiple sites. Causes of systemic inflammation remain poorly understood but molecules originating from the intestine likely play a role with microbial and host small molecules polarizing host immune cells towards a pro- or anti-inflammatory phenotype. Using the dextran sodium sulphate (DSS) mouse model, which mimics models the disrupted barrier function in IBD, microbial dysbiosis and immune cell dysregulation in IBD, we investigated metabolomic and phenotypic changes at intestinal and systemic sites. Through mass spectrometry imaging we mapped the spatial distribution and relative abundance of molecules and cell types across a range of tissues during colitis. This approach revealed specific molecular changes across a range of organs including the colon, ileum, liver, spleen and kidney, while no molecular changes were observed in the lungs of DSS-treated mice. Specific molecules, identified as contributing to the statistical separation of treated from control mice, were then spatially localized within organs to determine their effects on cellular phenotypes through imaging mass cytometry. Additionally, molecules that were significantly changed across multiple systemic sites in response to inflammation were identified. This spatial approach identified drivers of inflammation both locally in the intestine and systemically and has highlighted a number of molecules not previously implicated in inflammation linked to IBD or the systemic effects of intestinal inflammation. Together this data shows that gaining a better understanding of metabolic pathways and identifying molecular disease biomarkers within the intestine and systemic organs during IBD, might improve our understanding of disease aetiology and aid the development of new targeted therapies.

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Gamma-Linolenic Acid (GLA) Protects against Ionizing Radiation-Induced Damage: An In Vitro and In Vivo Study

Cited by 11 publications

References 86 publications

Pyridoxine, essential fatty acids, and protection against doxorubicin‐induced cardiotoxicity

Pyridoxine, essential fatty acids, and protection against doxorubicin‐induced cardiotoxicity

Tetrahedral framework nucleic acids alleviate irradiation‐induced salivary gland damage

Spatial mapping of dextran sodium sulphate-induced intestinal inflammation and its systemic effects

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