Gamma-melanocyte stimulating hormone regulates the expression and cellular localization of epithelial sodium channel in inner medullary collecting duct cells

Cope, Georgina; Kaushik, Gaurav; O’Sullivan, Siobhán; Healy, Vincent

doi:10.1016/j.peptides.2013.07.007

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…As this cell line is a mixture of different cells, retinoic acid (RA; Sigma) was used for differentiating SK-N-SH cells [23] into more neuronal cells [24]. Approximately 30,000 cells (per well in 6-well plates) were cutured in supplemented EMEM media for two days, followed by RA (10μM) treatment for two weeks and media was replaced every 3–4 days [23, 25]. Using light microscopy, differentiated neuronal cultures were validated with the presence of neuronal cell markers (NeuN, PSD95 and NCAM) during the differentiation process [23, 26] (Refer supplementary information).…”

Section: Methodsmentioning

confidence: 99%

Dysregulation of autism-associated synaptic proteins by psychoactive pharmaceuticals at environmental concentrations

Kaushik

Xia

Pfau

et al. 2017

Neuroscience Letters

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Autism Spectrum Disorders (ASD) are complex neurological disorders for which the prevalence in the U.S. is currently estimated to be 1 in 50 children. A majority of cases of idiopathic autism in children likely result from unknown environmental triggers in genetically susceptible individuals. These triggers may include maternal exposure of a developing embryo to environmentally relevant minute concentrations of psychoactive pharmaceuticals through ineffectively purified drinking water. Previous studies in our lab examined the extent to which gene sets associated with neuronal development were up- and down-regulated (enriched) in the brains of fathead minnows treated with psychoactive pharmaceuticals at environmental concentrations. The aim of this study was to determine whether similar treatments would alter in vitro expression of ASD-associated synaptic proteins on differentiated human neuronal cells. Human SK-N-SH neuroblastoma cells were differentiated for two weeks with 10μM retinoic acid (RA) and treated with environmentally relevant concentrations of fluoxetine, carbamazepine or venlafaxine, and flow cytometry technique was used to analyze expression of ASD-associated synaptic proteins. Data showed that carbamazepine individually, venlafaxine individually and mixture treatment at environmental concentrations significantly altered the expression of key synaptic proteins (NMDAR1, PSD95, SV2A, HTR1B, HTR2C and OXTR). Data indicated that psychoactive pharmaceuticals at extremely low concentrations altered the in vitro expression of key synaptic proteins that may potentially contribute to neurological disorders like ASD by disrupting neuronal development.

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Section: Methodsmentioning

confidence: 99%

Dysregulation of autism-associated synaptic proteins by psychoactive pharmaceuticals at environmental concentrations

Kaushik

Xia

Pfau

et al. 2017

Neuroscience Letters

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“…The major hereditary disorders are hemophilia, rare bleeding disorders and thrombosis. Acquired disorders are caused by several factors such as infections, habits and environmental effects [12].…”

Section: Defects In Hemostasismentioning

confidence: 99%

“…Chronic smoking and chronic alcoholism effect coagulation system by altering the liver functions. Environmental factors like hypoxia, drugs like aspirin, oral contraceptive pills, dietary problems like vitamin K deficiency affect the blood coagulation [12,[56][57][58][59][60][61].…”

Section: Acquired Coagulation Disordersmentioning

confidence: 99%

Understanding the Clotting Cascade, Regulators, and Clinical Modulators of Coagulation

Pilli¹

2018

Hematology - Latest Research and Clinical Advances

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The circulatory system plays a vital role in the survival of an organism by supplying it with essential nutrients, signaling molecules and eliminating the waste or toxic products from the body. This flow is tightly regulated by various factors, procoagulants support the formation of hemostatic plugs to prevent the leakage or blood loss and anticoagulants prevent the formation of unwanted clots. Disruption or dysregulation of procoagulants and anticoagulants lead to clinical complexities. In this chapter defects in the coagulation system, hereditary, acquired coagulation disorders, their diagnosis and recent clinical modulators of the coagulation system are discussed.

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Gamma-melanocyte stimulating hormone regulates the expression and cellular localization of epithelial sodium channel in inner medullary collecting duct cells

Cited by 2 publications

References 18 publications

Dysregulation of autism-associated synaptic proteins by psychoactive pharmaceuticals at environmental concentrations

Dysregulation of autism-associated synaptic proteins by psychoactive pharmaceuticals at environmental concentrations

Understanding the Clotting Cascade, Regulators, and Clinical Modulators of Coagulation

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