Gamma-oryzanol supplemented in extender enhances the quality of semen cryopreservation and alters proteomic profile in Thai swamp buffalo

Inyawilert, Wilasinee; Rungruangsak, Janyaporn; Liao, Yu-Jing; Wirojwutthikul, Sinchai; Phinyo, Mahattanee; Tang, Pin‐Chi; Wanangkarn, Amornrat; Tiantong, Attapol

doi:10.1016/j.cryobiol.2022.06.001

Cryobiology

2022

DOI: 10.1016/j.cryobiol.2022.06.001

|View full text |Cite

Gamma-oryzanol supplemented in extender enhances the quality of semen cryopreservation and alters proteomic profile in Thai swamp buffalo

Wilasinee Inyawilert

Janyaporn Rungruangsak²,

Yu-Jing Liao³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality

Inyawilert,

Liao,

Ndi

et al. 2024

Vet World

View full text Add to dashboard Cite

Background and Aim: The success of semen cryopreservation relies on several aspects, including breed, age, season, collection method, extender composition, cooling rate, equilibration period, freezing rate, and thawing rate. This study aimed to investigate the effects of cooling and equilibration duration, as well as the addition of antioxidants to the semen extender, on the cryopreservation of swamp buffalo semen. Materials and Methods: Semen collected from swamp buffalo bulls was subjected to four different conditions: (T1) 2-h cooling and 2-h equilibration, (T2) 1.5-h cooling and 1.5-h equilibration, (T3) 1-h cooling and 1-h equilibration, and (T4) 0.5-h cooling and 0.5-h equilibration. Spermatozoa motility was evaluated using a computer-assisted semen analyzer. Moreover, this study also investigated the effect of antioxidant supplementation during cryopreservation using tris-citrate egg yolk extenders enriched with various antioxidants: Control (Con), 1 mM melatonin (ML), 0.5 mM gamma-oryzanol (GO), 10 μM canthaxanthin (CX), 1 mM melatonin + 0.5 mM gamma-oryzanol (ML + GO), and 1 mM melatonin + 10 μM canthaxanthin (ML + CX). Results: Results showed that the (T1) 2-h cooling and 2-h equilibration and (T2) 1.5-h cooling and 1.5-h equilibration groups achieved higher progressive motility than the (T3) 1-h cooling and 1-h equilibration and (T4) 0.5-h cooling and 0.5-h equilibration groups. The ML-treated group exhibited superior progressive motility and total motility. Conclusion: The optimal approach for cryopreserving swamp buffalo bull semen involves a 1.5-h cooling period followed by a 1.5-h equilibration period, with the incorporation of ML into the semen extender. Keywords: antioxidant, cryopreservation, equilibration, semen, and swamp buffalo.

show abstract

The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality

Inyawilert,

Liao,

Ndi

et al. 2024

Vet World

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Gamma-oryzanol supplemented in extender enhances the quality of semen cryopreservation and alters proteomic profile in Thai swamp buffalo

Cited by 1 publication

References 59 publications

The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality

The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality

Contact Info

Product

Resources

About