Gamma radiation-induced grafting of n-hydroxyethyl acrylamide onto poly(3-hydroxybutyrate): A companion study on its polyurethane scaffolds meant for potential skin tissue engineering applications

Ochoa-Segundo, Eric Ivan; González‐Torres, Maykel; Cabrera-Wrooman, Alejandro; Sánchez-Sánchez, Roberto; Huerta-Martínez, B. M.; Melgarejo-Ramírez, Yaaziel; Leyva-Gómez, Gerardo; Rivera-Muñoz, Eric M.; Cortés, Hernán; Velasquillo, Cristina; Vargas-Muñoz, Susana; Rodríguez-Talavera, Rogelio

doi:10.1016/j.msec.2020.111176

Cited by 12 publications

(3 citation statements)

References 62 publications

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“…This phenomenon may result from the predominance of the concentration of PVP regarding XG (9:1). Nevertheless, in the XG-PVP spectrum, we observed a shift of those signals and decreased intensity, suggesting new covalent bonds in the polymeric chains (32,33). Likewise, the signal's dis-appearance at 1739 cm -1 indicates a probable formation of hydrogen bonds between the two polymers.…”

Section: Discussionmentioning

confidence: 77%

Physicochemical and biological characterization of a xanthan gum-polyvinylpyrrolidone hydrogel obtained by gamma irradiation

López-Huante

Prado-Audelo

Caballero-Florán

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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Xanthan gum (XG) and polyvinylpyrrolidone (PVP) are two polymers with low toxicity, high biocompatibility, biodegradability, and high hydrophilicity, making them promising candidates for multiple medical aspects. The present work aimed to synthesize a hydrogel from a mixture of XG and PVP and crosslinked by gamma irradiation. We assessed the hydrogel through a series of physicochemical (FT-IR, TGA, SEM, and percentage of swelling) and biological (stability of the hydrogel in cell culture medium) methods that allowed to determine its applicability. The structural evaluation by infrared spectrum demonstrated that a crosslinked hydrogel was obtained from the combination of polymers. The calorimetric test and swelling percentage confirmed the formation of the bonds responsible for the crosslinked structure. The calorimetric test evidenced that the hydrogel was resistant to decomposition in contrast to non-irradiated material.The determination of the swelling degree showed constant behavior over time, indicating a structure resistant to hydrolysis. This phenomenon also occurred during the test of stability in a cell culture medium. Additionally, microscopic analysis of the sample revealed an amorphous matrix with the presence of porosity. Thus, the findings reveal the synthesis of a novel material that has desirable attributes for its potential application in pharmaceutical and biomedical areas.

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Section: Discussionmentioning

confidence: 77%

Physicochemical and biological characterization of a xanthan gum-polyvinylpyrrolidone hydrogel obtained by gamma irradiation

López-Huante

Prado-Audelo

Caballero-Florán

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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“…Furthermore, gamma radiation-induced grafting of CS onto PHB was performed, and polyurethane composite scaffolds composed of PHB-g-CS and silica NPs were prepared by the salt-leaching method, and composite scaffolds showed good cytocompatibility [73]. Similarly, for skin tissue engineering, PHB-g-n-hydroxyethyl acrylamide (PHB-g-HEAA) as novel polyurethane scaffold showed good potential for wound dressing [74]. Furthermore, antioxidant and antimicrobial maleic anhydride-grafted PHBV (PHBV-g-MA)/fish scales (FSs) composites were prepared and processed in 3D printing filaments (as shown in Figure 9).…”

Section: Processing Of Pha-based Materialsmentioning

confidence: 99%

Bio-Based Sustainable Polymers and Materials: From Processing to Biodegradation

Okolie

Kumar

Edwards³

et al. 2023

J. Compos. Sci.

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In the life cycle of a material, there will be either chemical or physical change due to varying environmental factors such as biological activity, light, heat, moisture, and chemical conditions. This process leads to polymer property change as pertains to functional deterioration because of the physical, biological, and chemical reactions that result in chemical transformations and bond scission and thus can be regarded as polymer degradation. Due to the present demand for sustainable polymers, bio-based polymers have been identified as a solution. There is therefore a need to compare the sustainability impacts of bio-based polymers, to maximize their use in functional use stage and still withhold the bio-degradation capability. This study focuses are poly (lactic acid) (PLA), Poly (ε-caprolactone) (PCL), polyhydroxyalkanoates (PHA), and polyamides (PA) as biopolymers of interest due to their potential in technological applications, stability, and biodegradability. For preparing bio-based value-added products, an appropriate selection of the fabrication or functional modification process is a very important factor for particular industrial or biomedical applications. The literature review indicates that in vivo is preferred to in vitro because it suits an overall study of the experiment’s effects on a living subject. This study will explore these features in detail. In particular, the review will cover processing and biodegradation pathways for each of the biopolymers. In addition, thermal degredation and photodegradation are covered, and future trends and conclusions are drawn.

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“…These can be obtained by physical or chemical methods of poly-mers cross-linking, such as gamma irradiation (7,8). Cross-linking by gamma irradiation is an efficient method for hydrogels synthesis (9) because the gamma rays possess the required energy to ionize molecules either in the air or in water (10), generating reactive sites along the polymer chain, allowing the combination of these radicals, and leading to the formation of a large number of cross-links (7). Besides, this chemical modification improves the physicochemical and mechanical properties of the combined biopolymers, while their natural biocompatibility, biodegradability, and lack of immunogenicity are retained.…”

Section: Introductionmentioning

confidence: 99%

Radiation-induced graft polymerization of elastin onto polyvinylpyrrolidone as a possible wound dressing

Prado-Audelo

Gómez-Zaldivar

Pérez-Díaz

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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The purpose of our study was to obtain new wound dressings in the form of hydrogels that promote wound healing taking advantage of the broad activities of elastin (ELT) in physiological processes. The hydrogel of ELT and polyvinylpyrrolidone (PVP; ELT-PVP) was obtained by cross-linking induced by gamma irradiation at a dose of 25 kGy. The physicochemical changes attributed to cross-linking were analyzed through scanning electron microscopy (SEM), infrared spectroscopy analysis with Fourier transform (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Furthermore, we performed a rheological study to determine the possible changes in the fluidic macroscopic properties produced by the cross-linking method. Finally, we accomplished viability and proliferation analyses of human dermal fibroblasts in the presence of the hydrogel to evaluate its biological characteristics. The hydrogel exhibited a porous morphology, showing interconnected porous with an average pore size of 16 ± 8.42 µm. The analysis of FTIR, DSC, and TGA revealed changes in the chemical structure of the ELT-PVP hydrogel after the irradiation process. Also, the hydrogel exhibited a rheological behavior of a pseudoplastic and thixotropic fluid. The hydrogel was biocompatible, demonstrating high cell viability, whereas ELT presented low biocompatibility at high concentrations. In summary, the hydrogel obtained by gamma irradiation revealed the appropriate morphology to be applied as a wound dressing. Interestingly, the hydrogel exhibited a higher percentage of cell viability compared with ELT, suggesting that the cross-linking of ELT with PVP is a suitable strategy for biological applications of ELT without generating cellular damage.

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Gamma radiation-induced grafting of n-hydroxyethyl acrylamide onto poly(3-hydroxybutyrate): A companion study on its polyurethane scaffolds meant for potential skin tissue engineering applications

Cited by 12 publications

References 62 publications

Physicochemical and biological characterization of a xanthan gum-polyvinylpyrrolidone hydrogel obtained by gamma irradiation

Physicochemical and biological characterization of a xanthan gum-polyvinylpyrrolidone hydrogel obtained by gamma irradiation

Bio-Based Sustainable Polymers and Materials: From Processing to Biodegradation

Radiation-induced graft polymerization of elastin onto polyvinylpyrrolidone as a possible wound dressing

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