Gamma radiation induced variation in growth characteristics and production of bioactive compounds during callogenesis in Stevia rebaudiana (Bert.)

Khalil, Shahid Akbar; Ahmad, Nisar; Zamir, Roshan

doi:10.1016/j.neps.2015.06.002

Cited by 32 publications

(20 citation statements)

References 19 publications

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“…Gamma irradiation dosages that result in changes in germination and growth have specific decreasing effects on certain respiratory enzymes such as catalase, peroxidase, isocitric dehydrogenase, a-amylase, ribonuclease and 3 0 -nucleotidase. The levels of plant growth hormone indole acetic acid also decreased with increased irradiation dosages leading to slower growth and respiration rates (Abrol, Sirohi, & Sinha, 1969;Ananthaswamy et al, 1971;Khalil, Ahmad, & Zamir, 2015;Stoeva, 2002). Similarly, mitochondrial activity reduces with irradiation resulting in a drop in production of enzymes, which in turn results in reduced growth and development (Srivastava, 1973).…”

Section: Discussionmentioning

confidence: 99%

Efficiency of energy conversion and growth of gamma irradiated embryos and young seedlings of Triticum monococcum L. cultivar Einkorn

Well¹,

Fossey

Booyse³

2018

Journal of Radiation Research and Applied Sciences

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The study was conducted to determine whether the efficiency of energy conversion into growth can be used as an indicator for the determination of the optimal gamma irradiation dosage for mutation breeding. To meet this objective, embryo growth, shoot growth, root growth, mobilization of food reserves, respiration and energy conversion were studied in gamma-irradiated wheat Triticum monococcum L. cultivar Einkorn kernels. Kernels were exposed to 50, 150, 250 and 350 Gy and germinated. Kernels were collected 12 h after onset of imbibition and then every 12 h until 168 h. Irradiated seed demonstrated retardation in all parameters, which increased as the gamma irradiation dosage increased. For the most, dosage and time, as well as dosage by time interaction were highly significant. Root growth appeared to be the most sensitive to gamma irradiation, followed by shoot growth, mobilization of food reserves and efficiency of energy conversion. Full recovery of the efficiency of energy conversion took place at 50 Gy, with an increase in inefficiency with an increase in dosage. The point where full recovery of efficiency of energy conversion into growth gives way to incomplete recovery (100 Gy) is in line with the suggested dosages for practical mutation breeding in Triticum monococcum L. by the FAO/IAEA and is therefore an ideal indicator for predicting the dosage that will be optimal for plant mutation breeding.

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Section: Discussionmentioning

confidence: 99%

Efficiency of energy conversion and growth of gamma irradiated embryos and young seedlings of Triticum monococcum L. cultivar Einkorn

Well¹,

Fossey

Booyse³

2018

Journal of Radiation Research and Applied Sciences

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“…Moreover, Wi et al 27 observed that gamma irradiation remarkably increased the cerium perhydroxide in different tissues of pumpkin. Similarly, El-Beltagi (2011) reported gamma irradiation enhanced the production of antioxidant defense enzymes while reduced ascorbic acid, total soluble protein, amino acids and sugars in callus cultures of Rosmarinus officinalis L. The effect of gamma irradiation on callus biomass and biochemical parameters of Stevia rebaudiana Bertoni were reported by Khalil et al (2015) which can be helpful for the establishment of cell suspension culture, and production of bioactive compounds in bioreactors. Calli were transferred to fresh medium and exposed to various gamma irradiations (5.0, 10, 15 and 20 Gy).…”

Section: Resultsmentioning

confidence: 98%

Determination of Effective Mutagen Dose for Carrot (Daucus carota ssp. sativus var. atrorubens alef and D. carota) Callus Cultures

2019

JSTR

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Mutation breeding is used for plant variety, to obtain high yield, earliness, resistance to pest and diseases and also to abiotic stress conditions. Gamma radiation application in physical mutagen applications has advantages on the ease of use, the high permeability and the ability to reach the target cells and the nontoxic and harmful effects. It expanses the present variation by creating a mutation with physical mutagenesis via Co 60 irradiation applied to in vitro donor plant tissues, and provides selection of variations at the cellular level in desired properties. Carrot is an important vegetable crops and in demand by the local markets throughout the year. This study was carried out to obtain a database and to determine the Effective Mutagen Dose (EMD50) in the course of mutation breeding studies on purple and orange carrots. For this aim, 0, 5, 10, 20, 30, 40, 50 and 60 Gray (Gy) doses of Co 60 (gamma-rays) were applied to carrots calli as a physical mutagen of 30 callus pieces (each 1 g) per dose. 4-5 weeks after irradiation, shoot development of callus were determined and EMD50 calculated by linear regression analyses. As a result of the research, it was determined that 8,36 Gy was an effective mutation dose for purple carrot and 23,605 Gy for orange carrots due to the regression analyses.

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“…Also, El-Beltagi et al 66 found that low doses of gamma irradiation (15 and 20 Gy) enhanced the total phenolic and flavonoid accumulation in rosemary (Rosmarinus officinalis L.) callus cultures. Similarly, a 15 Gy dose of gamma irradiation slightly enhanced stevioside content over the control of Stevia rebaudiana callus cultures 67 . Gamma irradiation was also used for promoting some phytochemicals such as ascorbate, photosynthetic pigments and some antioxidant enzymes activity in red pepper (Capsicum annuum) when treated with gamma rays ranging from 2-16 Gy 68 .…”

Section: Enhancement Of Nutraceuticals Production By Mutationmentioning

confidence: 88%

In vitro Production of Plant Nutraceuticals

Bekheet¹,

Sota²

2022

Biotechnology

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Biotechnology methods particularly tissue culture techniques offer new approaches for the in vitro production of valuable plant nutraceuticals. The large-scale production of secondary metabolites in a year-round system without seasonal constraints is one of the advantages of plant tissue culture techniques. Furthermore, enhancement of in vitro nutraceuticals production can be realized by a selection of high-producing cell lines and medium optimization. In this respect, several techniques have been adopted to improve the production of plant-derived nutraceuticals such as mutation, elicitation, precursor feeding, genetic transformation and metabolic engineering. In vitro mutagenesis of cultured cells and tissues by irradiation or chemical mutagens represents a feasible method for the improvement of nutraceuticals production. Likewise, transformation is another strategy that is used to enhance nutraceuticals in plant cells or organ tissues. At this point, hairy root culture induced by Agrobacterium rhizogenes is the most promising transformation technique used for in vitro production of valuable plant compounds. The benefits of hairy roots are high metabolite productivity, high growth rates and inherent genetic stability. On the other hand, due to its effectiveness and practical feasibility, elicitation is considered the most applied strategy for enhancing the production of desired compounds in plant biotechnology. Biotic (biological origin) and abiotic (non-biological origin) elicitors are used to increase the production of plant metabolites by different effects on the cellular processes in the plant system. Furthermore, bioreactors developed for in vitro industrial-scale production of desired compounds can be used for continuous and scaling-up plant nutraceuticals production. This article discusses different aspects of biotechnology used for the selection and enhancement production of nutraceuticals from plant cultures grown cells, in vitro.

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Gamma radiation induced variation in growth characteristics and production of bioactive compounds during callogenesis in Stevia rebaudiana (Bert.)

Cited by 32 publications

References 19 publications

Efficiency of energy conversion and growth of gamma irradiated embryos and young seedlings of Triticum monococcum L. cultivar Einkorn

Efficiency of energy conversion and growth of gamma irradiated embryos and young seedlings of Triticum monococcum L. cultivar Einkorn

Determination of Effective Mutagen Dose for Carrot (Daucus carota ssp. sativus var. atrorubens alef and D. carota) Callus Cultures

In vitro Production of Plant Nutraceuticals

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