Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity

Abstract

Aspergillus oryzae was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. Mobilized polyphenols were used as a green tool for synthesis and stabilization of gold nanoparticles (AuNPs). The radiation-induced AuNPs synthesis is a simple, clean and inexpensive process which involves radiolysis of aqueous solution that provides an efficient method to reduce metal ions. Gamma irradiated aqueous extract of fermented soybean and garlic was used for rapid preparation of AuNPs combining both effects of radiolytic reactions by radiation and stabilization by bioactive components of fermented extract. The synthesized AuNPs were confirmed by UV-Visible spectrophotometry, dynamic light scattering (DLS), Fourier Transform infra red (FT-IR) spectrophotometry, and transmission electron microscope (TEM) analysis which revealed morphology of spherical AuNPs with size ranging from 7–12 nm. The synthesized AuNPs exhibited antimicrobial activity against both Gram positive and Gram negative bacteria, as measured by well diffusion assay.

Green chemistry approach for the synthesis of gold nanoparticles with gum kondagogu: characterization, catalytic and antibacterial activity

“…These results revealed that AuNPs synthesized from gum kondagogu demonstrated effective antibacterial activity in Gramnegative than in Gram-positive bacteria. The present results agree well with the work of other researchers [42]. It can be suggested that Gram-negative strains of bacteria (E. coli) with thin cell wall is more susceptible to cell wall damage compared to Gram-positive strain bacteria (B. subtilis) with a thick cell wall.…”

Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line

“…13 Figure 3(a) shows. Clove (Syzygium aromaticum) extract is after reaction with gold ions, this absorption band shows shift towards higher wave number with the loss in intensity [16] [17]. This observation indicates that eugenol molecules may be involved in the reduction of the surface of gold ion.…”

Synthesis and characterization of gold nanoparticles from Abies spectabilis extract and its anticancer activity on bladder cancer T24 cells

“…Although the rising attention being synthesis of AuNPs from natural plant extract, because the bio-reduction mechanism remains to have been elucidate. El-Batal et al [39] demonstrated that AuNPs synthesized from Antigonon leptopus leaf extract containing metabolites and biomolecules observed through FTIR analysis were responsible for the bioreduction of Au ions as well as in soybean-garlic aqueous extract have been reported. The different functional groups with their corresponding stretches obtained in the presently synthesized AuNPs are in agreement with the previous reported of Varshney et al [40].…”